Tumor Protein p53 Binding Protein 1 (TP53BP1)

53BP1; p53BP1; p202

SPN	P734
HGNC	11999
MGI	1351320
PubMed	21635553
RGD	1308039
UniProt	Q12888
Wiki	TP53BP1

TP53BP1 has a predicted molecular mass of 217 kD and shows homology in its C-terminal 247 amino acids to the C terminus of the tumor suppressor protein BRCA1. TP53BP1 was present in both the cytoplasm and nucleus in some cells and only in the nucleus in others. Furthermore, there were 2 nuclear staining patterns, one homogeneous staining and the other dot staining.TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. TP53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins BRCA1 and CHK2 but was required for the formation of BRCA1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.

Reference

"Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2."
J. Biol. Chem. 273:26061-26068(1998) [PubMed] [Europe PMC] [Abstract]

"The full-ORF clone resource of the German cDNA consortium."
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

"Analysis of the DNA sequence and duplication history of human chromosome 15."
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Two cellular proteins that bind to wild-type but not mutant p53."
Proc. Natl. Acad. Sci. U.S.A. 91:6098-6102(1994) [PubMed] [Europe PMC] [Abstract]

"Negative cell cycle regulation and DNA-damage inducible phosphorylation of the BRCT protein 53BP1."
J. Biol. Chem. 276:2708-2718(2001) [PubMed] [Europe PMC] [Abstract]

"Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways."
J. Cell Biol. 153:613-620(2001) [PubMed] [Europe PMC] [Abstract]

"53BP1, a mediator of the DNA damage checkpoint."
Science 298:1435-1438(2002) [PubMed] [Europe PMC] [Abstract]

"MDC1 is a mediator of the mammalian DNA damage checkpoint."
Nature 421:961-966(2003) [PubMed] [Europe PMC] [Abstract]

"p53-Binding protein 1 is fused to the platelet-derived growth factor receptor beta in a patient with a t(5;15)(q33;q22) and an imatinib-responsive eosinophilic myeloproliferative disorder."
Cancer Res. 64:7216-7219(2004) [PubMed] [Europe PMC] [Abstract]

"A pathway of double-strand break rejoining dependent upon ATM, Artemis, and proteins locating to gamma-H2AX foci."
Mol. Cell 16:715-724(2004) [PubMed] [Europe PMC] [Abstract]

"The GAR motif of 53BP1 is arginine methylated by PRMT1 and is necessary for 53BP1 DNA binding activity."
Cell Cycle 4:1834-1841(2005) [PubMed] [Europe PMC] [Abstract]

"53BP1 oligomerization is independent of its methylation by PRMT1."
Cell Cycle 4:1854-1861(2005) [PubMed] [Europe PMC] [Abstract]

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

"Characterisation of the sites of DNA damage-induced 53BP1 phosphorylation catalysed by ATM and ATR."
DNA Repair 6:1536-1544(2007) [PubMed] [Europe PMC] [Abstract]

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Protein phosphatase 5 regulates the function of 53BP1 after neocarzinostatin-induced DNA damage."
J. Biol. Chem. 284:9845-9853(2009) [PubMed] [Europe PMC] [Abstract]

"p8/nupr1 regulates DNA-repair activity after double-strand gamma irradiation-induced DNA damage."
J. Cell. Physiol. 221:594-602(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Regulation of chromatin architecture by the PWWP domain-containing DNA damage-responsive factor EXPAND1/MUM1."
Mol. Cell 37:854-864(2010) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Protein phosphatase 5 is necessary for ATR-mediated DNA repair."
Biochem. Biophys. Res. Commun. 404:476-481(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human 53BP1 BRCT domains bound to p53 tumour suppressor."
EMBO J. 21:3863-3872(2002) [PubMed] [Europe PMC] [Abstract]

"Structure of the 53BP1 BRCT region bound to p53 and its comparison to the Brca1 BRCT structure."
Genes Dev. 16:583-593(2002) [PubMed] [Europe PMC] [Abstract]

"Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks."
Nature 432:406-411(2004) [PubMed] [Europe PMC] [Abstract]

"Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair."
Cell 127:1361-1373(2006) [PubMed] [Europe PMC] [Abstract]