Sepiapterin Reductase (SPR)

SDR38C1; 7,8-Dihydrobiopterin:NADP+ Oxidoreductase; Short Chain Dehydrogenase/Reductase Family 38C,Member 1

SPN	P604
HGNC	11257
MGI	103078
PubMed	1883349
RGD	3753
UniProt	P35270
Wiki	SPR

Sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase; EC 1.1.1.153) catalyzes the NADPH-dependent reduction of various carbonyl substances, including derivatives of pteridines, and belongs to a group of enzymes called aldo-keto reductases. SPR plays an important role in the biosynthesis of tetrahydrobiopterin (BH4).

The clone encoded a protein of 261 amino acids with a calculated molecular mass of 28,047 Da. The predicted amino acid sequence of human sepiapterin reductase shows 74% identity with the rat enzyme and a striking homology with human carbonyl reductase, estradiol 17-beta-dehydrogenase, and 3-beta-hydroxy-5-ene steroid dehydrogenase, especially in the N-terminal region.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP604Hu01	Recombinant Sepiapterin Reductase (SPR)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP604Hu01	Monoclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IP.
Antibodies	PAP604Hu01	Polyclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IF.
Assay Kits	CLP604Hu01	CLIA Kit for Sepiapterin Reductase (SPR)	CLIA assay for Antigen Detection
Assay Kits	ELP604Hu01	ELISA Kit for Sepiapterin Reductase (SPR)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP604Mu01	Recombinant Sepiapterin Reductase (SPR)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP604Mu01	Monoclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IP.
Antibodies	PAP604Mu01	Polyclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IF.
Assay Kits	CLP604Mu01	CLIA Kit for Sepiapterin Reductase (SPR)	CLIA assay for Antigen Detection
Assay Kits	ELP604Mu01	ELISA Kit for Sepiapterin Reductase (SPR)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP604Ra01	Recombinant Sepiapterin Reductase (SPR)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP604Ra01	Monoclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IP.
Antibodies	PAP604Ra01	Polyclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IF.
Assay Kits	CLP604Ra01	CLIA Kit for Sepiapterin Reductase (SPR)	CLIA assay for Antigen Detection
Assay Kits	ELP604Ra01	ELISA Kit for Sepiapterin Reductase (SPR)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP604Ca01	Recombinant Sepiapterin Reductase (SPR)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP604Ca01	Monoclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IP.
Antibodies	PAP604Ca01	Polyclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IF.
Assay Kits	CLP604Ca01	CLIA Kit for Sepiapterin Reductase (SPR)	CLIA assay for Antigen Detection
Assay Kits	ELP604Ca01	ELISA Kit for Sepiapterin Reductase (SPR)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP604Po01	Recombinant Sepiapterin Reductase (SPR)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP604Po01	Monoclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IP.
Antibodies	PAP604Po01	Polyclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IF.
Assay Kits	CLP604Po01	CLIA Kit for Sepiapterin Reductase (SPR)	CLIA assay for Antigen Detection
Assay Kits	ELP604Po01	ELISA Kit for Sepiapterin Reductase (SPR)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP604Bo01	Recombinant Sepiapterin Reductase (SPR)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP604Bo01	Monoclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IP.
Antibodies	PAP604Bo01	Polyclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IF.
Assay Kits	CLP604Bo01	CLIA Kit for Sepiapterin Reductase (SPR)	CLIA assay for Antigen Detection
Assay Kits	ELP604Bo01	ELISA Kit for Sepiapterin Reductase (SPR)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP604Rb01	Recombinant Sepiapterin Reductase (SPR)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP604Rb01	Monoclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IP.
Antibodies	PAP604Rb01	Polyclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IF.
Assay Kits	CLP604Rb01	CLIA Kit for Sepiapterin Reductase (SPR)	CLIA assay for Antigen Detection
Assay Kits	ELP604Rb01	ELISA Kit for Sepiapterin Reductase (SPR)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPP604Mo01	Recombinant Sepiapterin Reductase (SPR)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAP604Mo01	Monoclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IP.
Antibodies	PAP604Mo01	Polyclonal Antibody to Sepiapterin Reductase (SPR)	WB; IHC; ICC; IF.
Assay Kits	CLP604Mo01	CLIA Kit for Sepiapterin Reductase (SPR)	CLIA assay for Antigen Detection
Assay Kits	ELP604Mo01	ELISA Kit for Sepiapterin Reductase (SPR)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Cloning and sequencing of cDNA encoding human sepiapterin reductase -- an enzyme involved in tetrahydrobiopterin biosynthesis."
Biochem. Biophys. Res. Commun. 179:183-189(1991) [PubMed] [Europe PMC] [Abstract]

"Detection of a novel sepiapterin reductase mRNA: assay of mRNA in various cells and tissues of various species."
Exp. Cell Res. 204:217-222(1993) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Genomic organization and chromosomal localization of the human sepiapterin reductase gene."
Biochem. Biophys. Res. Commun. 251:597-602(1998) [PubMed] [Europe PMC] [Abstract]

"Mutational analysis of sites in sepiapterin reductase phosphorylated by Ca2+/calmodulin-dependent protein kinase II."
Biochim. Biophys. Acta 1594:191-198(2002) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human sepiapterin reductase."
Submitted (FEB-2009) to the PDB data bank

"Mutations in the sepiapterin reductase gene cause a novel tetrahydrobiopterin-dependent monoamine-neurotransmitter deficiency without hyperphenylalaninemia."
Am. J. Hum. Genet. 69:269-277(2001) [PubMed] [Europe PMC] [Abstract]

"Sepiapterin reductase deficiency an autosomal recessive DOPA-responsive dystonia."
Mol. Genet. Metab. 89:116-120(2006) [PubMed] [Europe PMC] [Abstract]

"Dopa-responsive hypersomnia and mixed movement disorder due to sepiapterin reductase deficiency."
Neurology 67:2032-2035(2006) [PubMed] [Europe PMC] [Abstract]