Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1)
FDG; HMUDG; UNG3
SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair. The deduced 270-amino acid protein has a calculated molecular mass of about 30 kD. Human SMUG1 shares 60% identity with the Xenopus protein. Fluorescence-tagged SMUG1 localized predominantly to the nucleus of transiently transfected HeLa cells.
SMUG1 showed broader substrate specificity than UNG2, and AP endonuclease had a strong stimulatory effect on SMUG1 against double-stranded uracil, apparently due to enhance dissociation of SMUG1 from AP sites in double-stranded DNA. SMUG1 accumulated within nucleoli in cultured epithelial cells, while UNG2 was excluded from nucleoli.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP578Hu01 | Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP578Hu01 | Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IP. |
| PAP578Hu01 | Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP578Hu01 | CLIA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | CLIA assay for Antigen Detection |
| ELP578Hu01 | ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP578Mu01 | Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP578Mu01 | Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IP. |
| PAP578Mu01 | Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP578Mu01 | CLIA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | CLIA assay for Antigen Detection |
| ELP578Mu01 | ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP578Ra01 | Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP578Ra01 | Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IP. |
| PAP578Ra01 | Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP578Ra01 | CLIA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | CLIA assay for Antigen Detection |
| ELP578Ra01 | ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP578Ca01 | Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP578Ca01 | Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IP. |
| PAP578Ca01 | Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP578Ca01 | CLIA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | CLIA assay for Antigen Detection |
| ELP578Ca01 | ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP578Po01 | Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP578Po01 | Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IP. |
| PAP578Po01 | Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP578Po01 | CLIA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | CLIA assay for Antigen Detection |
| ELP578Po01 | ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP578Bo01 | Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP578Bo01 | Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IP. |
| PAP578Bo01 | Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP578Bo01 | CLIA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | CLIA assay for Antigen Detection |
| ELP578Bo01 | ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP578Rb01 | Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP578Rb01 | Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IP. |
| PAP578Rb01 | Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP578Rb01 | CLIA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | CLIA assay for Antigen Detection |
| ELP578Rb01 | ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP578Mo01 | Recombinant Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP578Mo01 | Monoclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IP. |
| PAP578Mo01 | Polyclonal Antibody to Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP578Mo01 | CLIA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | CLIA assay for Antigen Detection |
| ELP578Mo01 | ELISA Kit for Single Strand Selective Monofunctional Uracil DNA Glycosylase 1 (SMUG1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors."
Curr. Biol. 9:174-185(1999) [PubMed] [Europe PMC] [Abstract]
"Mammalian 5-formyluracil-DNA glycosylase. 2. Role of SMUG1 uracil-DNA glycosylase in repair of 5-formyluracil and other oxidized and deaminated base lesions."
Biochemistry 42:5003-5012(2003) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Definitive identification of mammalian 5-hydroxymethyluracil DNA N-glycosylase activity as SMUG1."
J. Biol. Chem. 276:41991-41997(2001) [PubMed] [Europe PMC] [Abstract]
"hUNG2 is the major repair enzyme for removal of uracil from U:A matches, U:G mismatches, and U in single-stranded DNA, with hSMUG1 as a broad specificity backup."
J. Biol. Chem. 277:39926-39936(2002) [PubMed] [Europe PMC] [Abstract]
"Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase."
Nucleic Acids Res. 32:5291-5302(2004) [PubMed] [Europe PMC] [Abstract]