Riboflavin Kinase (RFK)

RIFK; Flavokinase; ATP:riboflavin 5'-phosphotransferase

SPN	P508
HGNC	30324
MGI	1914688
PubMed	19641494
RGD	1563242
UniProt	Q969G6
Wiki	RFK

Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis.

The reference genome sequence contains a polymorphism that changes the upstream ATG found in most transcripts to ATT; hence the next downstream AUG is annotated. This polymorphism is represented in the Reference SNP, rs2490582. The reference genome polymorphism results in a protein that is 7 aa shorter at the N-terminus, and is supported by homologous proteins. It is also possible that leaky scanning by ribosomes would permit translation initiation from the downstream AUG in alleles containing the upstream AUG.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	P508Hu01	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	MP508Hu21	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	PP508Hu01	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	KEP508Hu	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Chicken (Gallus)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Ovis aries; Ovine (Sheep)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Cavia (Guinea pig )

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Equus caballus; Equine (Horse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Capra hircus; Caprine (Goat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Organism species: Pan-species (General)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	NONE	Recombinant Riboflavin Kinase (RFK)	Positive Control; Immunogen; SDS-PAGE; WB.
Proteins	NONE	Native Riboflavin Kinase (RFK)	Bioactivity
Antibodies	NONE	Monoclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IP.
	NONE	Polyclonal Antibody to Riboflavin Kinase (RFK)	WB; IHC; ICC; IF.
	NONE	Biotin Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	ELISA(Det);IC; IH; IH(P); WB
	NONE	APC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	IHC, WB
	NONE	HRP Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Simple Western; WB
	NONE	PE Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry
	NONE	FITC Linked-Polyclonal Antibody to Riboflavin Kinase (RFK)	Flow Cytometry;ICC/IF
Assay Kits	NONE	ELISA Kit for Riboflavin Kinase (RFK)	Enzyme-linked immunosorbent assay for Antigen Detection
Assay Kits	NONE	CLIA kit for Riboflavin Kinase (RFK)	CLIA assay for Antigen Detection

Reference

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"DNA sequence and analysis of human chromosome 9."
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Riboflavin kinase couples TNF receptor 1 to NADPH oxidase."
Nature 460:1159-1163(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch."
Structure 11:265-273(2003) [PubMed] [Europe PMC] [Abstract]