Protein Kinase, cGMP Dependent Type I (PRKG1)

CGKI; PGK; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha; cGMP-dependent protein kinase 1

SPN	P428
HGNC	9414
MGI	108174
PubMed	23910461
UniProt	Q13976
Wiki	PRKG1

Two main forms of cGK have been identified: a soluble form designated type I and an intrinsic membrane-bound form designated type II.

Prkg1 knockout mice showed impaired platelet responses to VWF or low doses of thrombin and prolonged bleeding time. Human platelet aggregation induced by VWF or low-dose thrombin was inhibited by PRKG1 inhibitors but enhanced by cGMP. Furthermore, a cGMP-enhancing agent, sildenafil, promoted VWF- or thrombin-induced platelet aggregation. The cGMP-stimulated platelet responses were biphasic, consisting of an initial transient stimulatory response that promoted platelet aggregation and a subsequent inhibitory response that limited the size of thrombi.

Reference

"Molecular cloning and predicted full-length amino acid sequence of the type I beta isozyme of cGMP-dependent protein kinase from human placenta. Tissue distribution and developmental changes in rat."
FEBS Lett. 255:321-329(1989) [PubMed] [Europe PMC] [Abstract]

"cDNA cloning and gene expression of human type Ialpha cGMP-dependent protein kinase."
Hypertension 27:552-557(1996) [PubMed] [Europe PMC] [Abstract]

"Characterization of the human gene encoding the type I alpha and type I beta cGMP-dependent protein kinase (PRKG1)."
Genomics 42:311-318(1997) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The full-ORF clone resource of the German cDNA consortium."
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and comparative analysis of human chromosome 10."
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"cAMP- and cGMP-dependent protein kinase phosphorylation sites of the focal adhesion vasodilator-stimulated phosphoprotein (VASP) in vitro and in intact human platelets."
J. Biol. Chem. 269:14509-14517(1994) [PubMed] [Europe PMC] [Abstract]

"Regulation of myosin phosphatase by a specific interaction with cGMP-dependent protein kinase Ialpha."
Science 286:1583-1587(1999) [PubMed] [Europe PMC] [Abstract]

"cGMP-dependent protein kinase phosphorylates and inactivates RhoA."
Biochem. Biophys. Res. Commun. 280:798-805(2001) [PubMed] [Europe PMC] [Abstract]

"cGMP-dependent protein kinase I beta physically and functionally interacts with the transcriptional regulator TFII-I."
J. Biol. Chem. 277:32003-32014(2002) [PubMed] [Europe PMC] [Abstract]

"Regulation of cGMP-specific phosphodiesterase (PDE5) phosphorylation in smooth muscle cells."
J. Biol. Chem. 277:3310-3317(2002) [PubMed] [Europe PMC] [Abstract]

"Regulator of G-protein signaling-2 mediates vascular smooth muscle relaxation and blood pressure."
Nat. Med. 9:1506-1512(2003) [PubMed] [Europe PMC] [Abstract]

"Smooth muscle phosphatase is regulated in vivo by exclusion of phosphorylation of threonine 696 of MYPT1 by phosphorylation of Serine 695 in response to cyclic nucleotides."
J. Biol. Chem. 279:34496-34504(2004) [PubMed] [Europe PMC] [Abstract]

"IRAG mediates NO/cGMP-dependent inhibition of platelet aggregation and thrombus formation."
Blood 109:552-559(2007) [PubMed] [Europe PMC] [Abstract]

"Interactions between the leucine-zipper motif of cGMP-dependent protein kinase and the C-terminal region of the targeting subunit of myosin light chain phosphatase."
J. Mol. Biol. 373:1198-1212(2007) [PubMed] [Europe PMC] [Abstract]

"Probing the interaction between the coiled coil leucine zipper of cGMP-dependent protein kinase Ialpha and the C terminus of the myosin binding subunit of the myosin light chain phosphatase."
J. Biol. Chem. 283:32860-32869(2008) [PubMed] [Europe PMC] [Abstract]

"Large-scale proteomics analysis of the human kinome."
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

"cGMP-dependent protein kinases and cGMP phosphodiesterases in nitric oxide and cGMP action."
Pharmacol. Rev. 62:525-563(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Functional regulation of transient receptor potential canonical 7 by cGMP-dependent protein kinase Ialpha."
Cell. Signal. 23:1179-1187(2011) [PubMed] [Europe PMC] [Abstract]

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

"Rapid and accurate structure determination of coiled-coil domains using NMR dipolar couplings: application to cGMP-dependent protein kinase Ialpha."
Protein Sci. 14:2421-2428(2005) [PubMed] [Europe PMC] [Abstract]

"A crystal structure of the cyclic GMP-dependent protein kinase I{beta} dimerization/docking domain reveals molecular details of isoform-specific anchoring."
J. Biol. Chem. 285:32684-32688(2010) [PubMed] [Europe PMC] [Abstract]

"Co-crystal structures of PKG Ibeta (92-227) with cGMP and cAMP reveal the molecular details of cyclic-nucleotide binding."
PLoS ONE 6:E18413-E18413(2011) [PubMed] [Europe PMC] [Abstract]

"Patterns of somatic mutation in human cancer genomes."
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]

"Recurrent gain-of-function mutation in PRKG1 causes thoracic aortic aneurysms and acute aortic dissections."
Am. J. Hum. Genet. 93:398-404(2013) [PubMed] [Europe PMC] [Abstract]