Phosphohistidine Phosphatase 1 (PHPT1)

PHP14; Phosphohistidine Phosphatase 14kDa; Sex-Regulated Protein Janus-a; Protein janus-A homolog

SPN	P331
HGNC	30033
MGI	1922704
PubMed	19380421
RGD	1311355
UniProt	Q9NRX4
Wiki	PHPT1

PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I . PHPT1 gene contains 3 exons.

The deduced 125-amino acid PHPT1 protein migrated as a single, approximately 14 kD band by SDS-PAGE. Northern blot analysis detected a 0.6-kb transcript, with highest expression in heart and skeletal muscle and lower expression in several other tissues including pancreas, liver, and kidney.recombinant PHPT1 overexpressed and purified from bacteria displayed specific phosphohistidine phosphatase activity toward phosphopeptide I. No activity was detected towards phosphoserine, phosphothreonine, or phosphotyrosine peptides.

Reference

"Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase."
Eur. J. Biochem. 269:5016-5023(2002) [PubMed] [Europe PMC] [Abstract]

"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."
Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]

"Identification of the human crooked neck gene by comparative gene identification."
Biochim. Biophys. Acta 1517:449-454(2001) [PubMed] [Europe PMC] [Abstract]

"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs."
Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]

"DNA sequence and analysis of human chromosome 9."
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Mutational study of human phosphohistidine phosphatase: effect on enzymatic activity."
Biochem. Biophys. Res. Commun. 337:887-891(2005) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"First structure of a eukaryotic phosphohistidine phosphatase."
J. Biol. Chem. 281:33830-33834(2006) [PubMed] [Europe PMC] [Abstract]

"Solution structure and catalytic mechanism of human protein histidine phosphatase 1."
Biochem. J. 418:337-344(2009) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human phosphohistidine phosphatase. Northeast structural genomics consortium target HR1409."
Submitted (JAN-2007) to the PDB data bank