Neuroepithelial Cell Transforming Gene 1 (NET1)

ARHGEF8; NET1A; Proto-oncogene p65 Net1; Rho guanine nucleotide exchange factor 8

SPN	P178
HGNC	14592
MGI	1927138
PubMed	21390328
RGD	1305034
UniProt	Q7Z628
Wiki	NET1

By expression cloning and PCR of a neuroepithelioma cell line, followed by screening of a mammary epithelial cell line, Chan et al. (1996) obtained a cDNA encoding NET1. Sequence analysis predicted that the 601-amino acid protein has a DBL homology (DH) domain that is 21% similar to that of DBL, particularly in the leucine-rich region. The DH domain is a 240-amino acid region initially identified in DBL. DH domain proteins interact with members of the RAS superfamily of GTP-binding proteins. NET1 also possesses a pleckstrin-homology domain. Northern blot analysis revealed ubiquitous expression of major 3.0- and minor 2.4-kb NET1 transcripts, with highest levels in placenta. Functional analysis showed that NET1 induced morphologic alterations and conferred a malignant phenotype in vitro and in nude mice.

Reference

"Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning."
Oncogene 12:1259-1266(1996) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"The activity of guanine exchange factor NET1 is essential for transforming growth factor-beta-mediated stress fiber formation."
J. Biol. Chem. 276:15362-15368(2001) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage."
PLoS ONE 6:E17108-E17108(2011) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of the RhoGEF domain of human neuroepithelial cell-transforming gene 1 protein."
Submitted (FEB-2009) to the PDB data bank

"The consensus coding sequences of human breast and colorectal cancers."
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]