Never In Mitosis Gene A Related Kinase 2 (NEK2)

HsPK 21; HsPK21; NEK2A; NLK1; NimA-like protein kinase 1; Serine/threonine-protein kinase Nek2

SPN	P174
HGNC	7745
MGI	109359
PubMed	24018644
UniProt	P51955
Wiki	NEK2

NEK2 has an N-terminal catalytic domain and a very basic C-terminal region. Overall, NEK2 shares 36 to 38% sequence identity with nimA, Nek1, and human NEK3. Using Western blots of extracts of synchronized HeLa cells, the authors found that NEK2 protein was almost undetectable during G1, but accumulated progressively throughout S phase, reaching maximal levels in late G2.

NEK2B encodes a deduced 384-amino acid protein with a calculated molecular mass of 44.9 kD. It diverges from the longer isoform, NEK2A, following amino acid 370 and lacks a number of important regulatory motifs in the extreme C terminus of NEK2A. The 2 isoforms exhibited distinct patterns of cell cycle-dependent expression in U2OS cells.

Reference

"Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans."
Cell Growth Differ. 5:625-635(1994) [PubMed] [Europe PMC] [Abstract]

"Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis."
Biochem. J. 361:77-85(2002) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans."
Cell Growth Differ. 4:821-830(1993) [PubMed] [Europe PMC] [Abstract]

"Alternatively spliced protein variants as potential therapeutic targets for male infertility and contraception."
Ann. N. Y. Acad. Sci. 1030:468-478(2004) [PubMed] [Europe PMC] [Abstract]

"NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling."
J. Biol. Chem. 279:20049-20057(2004) [PubMed] [Europe PMC] [Abstract]

"Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles."
Mol. Biol. Cell 14:2876-2889(2003) [PubMed] [Europe PMC] [Abstract]

"Nek2A specifies the centrosomal localization of Erk2."
Biochem. Biophys. Res. Commun. 321:495-501(2004) [PubMed] [Europe PMC] [Abstract]

"Nek2A kinase regulates the localization of numatrin to centrosome in mitosis."
FEBS Lett. 575:112-118(2004) [PubMed] [Europe PMC] [Abstract]

"Nucleolar Nek11 is a novel target of Nek2A in G1/S-arrested cells."
J. Biol. Chem. 279:32716-32727(2004) [PubMed] [Europe PMC] [Abstract]

"Protein phosphatase-1alpha regulates centrosome splitting through Nek2."
Cancer Res. 67:1082-1089(2007) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation of human Sgo1 by NEK2A is essential for chromosome congression in mitosis."
Cell Res. 17:608-618(2007) [PubMed] [Europe PMC] [Abstract]

"Alternative splicing controls nuclear translocation of the cell cycle-regulated Nek2 kinase."
J. Biol. Chem. 282:26431-26440(2007) [PubMed] [Europe PMC] [Abstract]

"beta-Catenin is a Nek2 substrate involved in centrosome separation."
Genes Dev. 22:91-105(2008) [PubMed] [Europe PMC] [Abstract]

"Phosphoproteome of resting human platelets."
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]

"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]

"The mitotic checkpoint kinase NEK2A regulates kinetochore microtubule attachment stability."
Oncogene 27:4107-4114(2008) [PubMed] [Europe PMC] [Abstract]

"An autoinhibitory tyrosine motif in the cell-cycle-regulated Nek7 kinase is released through binding of Nek9."
Mol. Cell 36:560-570(2009) [PubMed] [Europe PMC] [Abstract]

"Large-scale proteomics analysis of the human kinome."
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]

"Involvement of a centrosomal protein kendrin in the maintenance of centrosome cohesion by modulating Nek2A kinase activity."
Biochem. Biophys. Res. Commun. 398:217-223(2010) [PubMed] [Europe PMC] [Abstract]

"Nek2 targets the mitotic checkpoint proteins Mad2 and Cdc20: a mechanism for aneuploidy in cancer."
Exp. Mol. Pathol. 88:225-233(2010) [PubMed] [Europe PMC] [Abstract]

"Components of the Hippo pathway cooperate with Nek2 kinase to regulate centrosome disjunction."
Nat. Cell Biol. 12:1166-1176(2010) [PubMed] [Europe PMC] [Abstract]

"An undecided coiled-coil: the leucine zipper of NEK2 kinase exhibits atypical conformational exchange dynamics."
J. Biol. Chem. 286:27537-27547(2011) [PubMed] [Europe PMC] [Abstract]

"Caught Nek-ing: cilia and centrioles."
J. Cell Sci. 118:5161-5169(2005) [PubMed] [Europe PMC] [Abstract]

"Whole genome sequencing in patients with retinitis pigmentosa reveals pathogenic DNA structural changes and NEK2 as a new disease gene."
Proc. Natl. Acad. Sci. U.S.A. 110:16139-16144(2013) [PubMed] [Europe PMC] [Abstract]

"Structure and regulation of the human Nek2 centrosomal kinase."
J. Biol. Chem. 282:6833-6842(2007) [PubMed] [Europe PMC] [Abstract]

"Patterns of somatic mutation in human cancer genomes."
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]