Gamma-Glutamyl Hydrolase (gGH)

GH; conjugase, folylpolygammaglutamyl hydrolase; Gamma-Glu-X carboxypeptidase

SPN	O715
HGNC	4248
MGI	1329035
PubMed	23374458
RGD	2682
UniProt	Q92820
Wiki	GGH

GGH catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate.The cDNA encodes a 318-amino acid protein with a deduced amino acid sequence 67% identical to that of the rat enzyme. The N-terminal 24 residues are likely a leader sequence that mediates translocation of GGH into the endoplasmic reticulum for secretion. GGH also contains 4 potential N-glycosylation sites. Western blot analysis detected GGH at an apparent molecular mass of about 35 kD.human GGH showed higher activity toward the pentaglutamate derivative of methotrexate and had little activity against the diglutamate derivative. More than 60% of the total GGH activity was secreted into the medium of the 5 tumor cell lines examined.

Reference

"Human gamma-glutamyl hydrolase: cloning and characterization of the enzyme expressed in vitro."
Proc. Natl. Acad. Sci. U.S.A. 93:10134-10138(1996) [PubMed] [Europe PMC] [Abstract]

"Structural organization of the human gamma-glutamyl hydrolase gene."
Gene 238:463-470(1999) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Site-directed mutagenesis establishes cysteine-110 as essential for enzyme activity in human gamma-glutamyl hydrolase."
Biochem. J. 343:551-555(1999) [PubMed] [Europe PMC] [Abstract]

"Molecular modeling and site-directed mutagenesis define the catalytic motif in human gamma -glutamyl hydrolase."
J. Biol. Chem. 275:40365-40370(2000) [PubMed] [Europe PMC] [Abstract]

"Proteomic analysis of early melanosomes: identification of novel melanosomal proteins."
J. Proteome Res. 2:69-79(2003) [PubMed] [Europe PMC] [Abstract]

"Characterization of human gamma-glutamyl hydrolase in solution demonstrates that the enzyme is a non-dissociating homodimer."
Biochim. Biophys. Acta 1764:1479-1486(2006) [PubMed] [Europe PMC] [Abstract]

"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Three-dimensional structure of human gamma -glutamyl hydrolase. A class I glutamine amidotransferase adapted for a complex substate."
J. Biol. Chem. 277:24522-24529(2002) [PubMed] [Europe PMC] [Abstract]