Exosome Component 2 (EXOSC2)

RRP4; Rrp4p; hRrp4p; p7; Ribosomal RNA-processing protein 4

SPN	O627
HGNC	17097
MGI	2385133
PubMed	9390555
RGD	1306573
UniProt	Q13868
Wiki	EXOSC2

Mammalian mRNAs contain AU-rich elements (AREs) within their three prime untranslated regions. In yeast, 3-prime-to-5-prime mRNA degradation is mediated by the exosome, a multisubunit particle. EXOSC2 (which is homologous to the yeast Rrp4 protein) is a component of the human exosome.

The protein encoded by this gene is required for a 3-prime to 5-prime exonuclease activity that generates the 3-prime end of the 5.8S rRNA. The yeast protein, as well as its human homolog, is only 1 enzymatic subunit of a multienzyme ribonuclease complex that Mitchell et al. (1997) referred to as the 'exosome.' The human protein is 43% identical to the yeast protein. Expression of the human RRP4 gene in yeast complemented the mutation in the rrp4.1 strain.

Reference

"Sequence of the human abl and bcr genes."
Thesis (1994), University of Oklahoma, United States

"The exosome: a conserved eukaryotic RNA processing complex containing multiple 3'-->5' exoribonucleases."
Cell 91:457-466(1997) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"DNA sequence and analysis of human chromosome 9."
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"The 3' end of yeast 5.8S rRNA is generated by an exonuclease processing mechanism."
Genes Dev. 10:502-513(1996) [PubMed] [Europe PMC] [Abstract]

"The yeast exosome and human PM-Scl are related complexes of 3'-->5' exonucleases."
Genes Dev. 13:2148-2158(1999) [PubMed] [Europe PMC] [Abstract]

"AU binding proteins recruit the exosome to degrade ARE-containing mRNAs."
Cell 107:451-464(2001) [PubMed] [Europe PMC] [Abstract]

"Human cell growth requires a functional cytoplasmic exosome, which is involved in various mRNA decay pathways."
RNA 13:1027-1035(2007) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Dis3-like 1: a novel exoribonuclease associated with the human exosome."
EMBO J. 29:2358-2367(2010) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Modulation of exosome-mediated mRNA turnover by interaction of GTP-binding protein 1 (GTPBP1) with its target mRNAs."
FASEB J. 25:2757-2769(2011) [PubMed] [Europe PMC] [Abstract]

"Reconstitution, activities, and structure of the eukaryotic RNA exosome."
Cell 127:1223-1237(2006) [PubMed] [Europe PMC] [Abstract]

Erratum
Cell 131:188-189(2007)