Debranching Enzyme Homolog 1 (DBR1)

Lariat Debranching Enzyme

SPN	O453
HGNC	15594
MGI	1931520
PubMed	16232320
RGD	1598038
UniProt	Q9UK59
Wiki	DBR1

The RNA lariat debranching enzyme, or DBR1, specifically hydrolyzes 2-prime-to-5-prime branched phosphodiester bonds at the branch point of excised lariat intron RNA and converts them into linear molecules.

The deduced 545-amino acid protein is approximately 42% identical to the fission yeast, budding yeast, and worm enzymes, and is 79% identical to the mouse enzyme. It contains a conserved N terminus, especially within the first 200 residues. Immunoblot analysis showed expression of a 62-kD protein with debranching activity. EST database searching suggested that DBR1 is of extremely low abundance, may be enriched in certain tissues, and has high specific enzymatic activity. Complementation analysis indicated that DBR1 is functional in yeast.

Reference

"Human RNA lariat debranching enzyme cDNA complements the phenotypes of Saccharomyces cerevisiae dbr1 and Schizosaccharomyces pombe dbr1 mutants."
Nucleic Acids Res. 28:3666-3673(2000) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Purification of a RNA debranching activity from HeLa cells."
J. Biol. Chem. 262:4274-4279(1987) [PubMed] [Europe PMC] [Abstract]

"DBR1 siRNA inhibition of HIV-1 replication."
Retrovirology 2:63-63(2005) [PubMed] [Europe PMC] [Abstract]

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]