Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides (DAPP1)

BAM32; B lymphocyte adapter protein Bam32; B-cell adapter molecule of 32 kDa; Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide

SPN	O450
HGNC	16500
MGI	1347063
PubMed	11513726
RGD	1310189
UniProt	Q9UN19
Wiki	DAPP1

Adaptor proteins are a link between 'upstream' cell surface receptors and nonreceptor protein tyrosine kinases and 'downstream' molecules such as phosphatidylinositol (PtdIns) 3-kinase (PI3K).Sequence analysis predicted that the 280-amino acid protein contains a potential myristoylation site (a glycine after the N-terminal methionine), an N-terminal SH2 domain, and a C-terminal PH domain with a PtdIns-interacting motif. The authors noted that APS also has an SH2 domain and a PH domain but at opposite termini of the protein. PCR of cDNA libraries and Northern blot analysis revealed ubiquitous expression of 2.7-kb transcripts, with highest levels in placenta and lung. A protein-lipid overlay analysis indicated that the PH domain of DAPP1 interacts with physiologic enantiomers of PtdIns.

Reference

"DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides."
Biochem. J. 342:7-12(1999) [PubMed] [Europe PMC] [Abstract]

"A novel B lymphocyte-associated adaptor protein, Bam32, regulates antigen receptor signaling downstream of phosphatidylinositol 3-kinase."
J. Exp. Med. 191:1319-1332(2000) [PubMed] [Europe PMC] [Abstract]

"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"The full-ORF clone resource of the German cDNA consortium."
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

"Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains."
Mol. Cell 6:373-384(2000) [PubMed] [Europe PMC] [Abstract]