Catechol-O-Methyltransferase (COMT)

SPN	O382
HGNC	2228
MGI	88470
PubMed	22528689
RGD	2379
UniProt	P21964
Wiki	COMT

COMT is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines.

Catechol-O-methyl transferase is involved in the inactivation of the catecholamine neurotransmitters (dopamine, epinephrine, and norepinephrine). The enzyme introduces a methyl group to the catecholamine, which is donated by S-adenosyl methionine (SAM). COMT is an intracellular enzyme located in the postsynaptic neuron. Any compound having a catechol structure, like catecholestrogens and catechol-containing flavonoids, are substrates of COMT.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPO382Hu01	Recombinant Catechol-O-Methyltransferase (COMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAO382Hu01	Monoclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IP.
Antibodies	PAO382Hu01	Polyclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IF.
Assay Kits	CLO382Hu01	CLIA Kit for Catechol-O-Methyltransferase (COMT)	CLIA assay for Antigen Detection
Assay Kits	ELO382Hu01	ELISA Kit for Catechol-O-Methyltransferase (COMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPO382Mu01	Recombinant Catechol-O-Methyltransferase (COMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAO382Mu01	Monoclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IP.
Antibodies	PAO382Mu01	Polyclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IF.
Assay Kits	CLO382Mu01	CLIA Kit for Catechol-O-Methyltransferase (COMT)	CLIA assay for Antigen Detection
Assay Kits	ELO382Mu01	ELISA Kit for Catechol-O-Methyltransferase (COMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPO382Ra01	Recombinant Catechol-O-Methyltransferase (COMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAO382Ra01	Monoclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IP.
Antibodies	PAO382Ra01	Polyclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IF.
Assay Kits	CLO382Ra01	CLIA Kit for Catechol-O-Methyltransferase (COMT)	CLIA assay for Antigen Detection
Assay Kits	ELO382Ra01	ELISA Kit for Catechol-O-Methyltransferase (COMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPO382Ca01	Recombinant Catechol-O-Methyltransferase (COMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAO382Ca01	Monoclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IP.
Antibodies	PAO382Ca01	Polyclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IF.
Assay Kits	CLO382Ca01	CLIA Kit for Catechol-O-Methyltransferase (COMT)	CLIA assay for Antigen Detection
Assay Kits	ELO382Ca01	ELISA Kit for Catechol-O-Methyltransferase (COMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPO382Po01	Recombinant Catechol-O-Methyltransferase (COMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAO382Po01	Monoclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IP.
Antibodies	PAO382Po01	Polyclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IF.
Assay Kits	CLO382Po01	CLIA Kit for Catechol-O-Methyltransferase (COMT)	CLIA assay for Antigen Detection
Assay Kits	ELO382Po01	ELISA Kit for Catechol-O-Methyltransferase (COMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPO382Bo01	Recombinant Catechol-O-Methyltransferase (COMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAO382Bo01	Monoclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IP.
Antibodies	PAO382Bo01	Polyclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IF.
Assay Kits	CLO382Bo01	CLIA Kit for Catechol-O-Methyltransferase (COMT)	CLIA assay for Antigen Detection
Assay Kits	ELO382Bo01	ELISA Kit for Catechol-O-Methyltransferase (COMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPO382Rb01	Recombinant Catechol-O-Methyltransferase (COMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAO382Rb01	Monoclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IP.
Antibodies	PAO382Rb01	Polyclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IF.
Assay Kits	CLO382Rb01	CLIA Kit for Catechol-O-Methyltransferase (COMT)	CLIA assay for Antigen Detection
Assay Kits	ELO382Rb01	ELISA Kit for Catechol-O-Methyltransferase (COMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPO382Mo01	Recombinant Catechol-O-Methyltransferase (COMT)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAO382Mo01	Monoclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IP.
Antibodies	PAO382Mo01	Polyclonal Antibody to Catechol-O-Methyltransferase (COMT)	WB; IHC; ICC; IF.
Assay Kits	CLO382Mo01	CLIA Kit for Catechol-O-Methyltransferase (COMT)	CLIA assay for Antigen Detection
Assay Kits	ELO382Mo01	ELISA Kit for Catechol-O-Methyltransferase (COMT)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Cloning and characterization of human placental catechol-O-methyltransferase cDNA."
DNA Cell Biol. 10:181-189(1991) [PubMed] [Europe PMC] [Abstract]

"Human catechol-O-methyltransferase: cloning and expression of the membrane-associated form."
Proc. Natl. Acad. Sci. U.S.A. 88:1416-1420(1991) [PubMed] [Europe PMC] [Abstract]

"Genomic organization of the human catechol O-methyltransferase gene and its expression from two distinct promoters."
Eur. J. Biochem. 223:1049-1059(1994) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"A genome annotation-driven approach to cloning the human ORFeome."
Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence of human chromosome 22."
Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Mass spectrometric analysis of human soluble catechol O-methyltransferase expressed in Escherichia coli. Identification of a product of ribosomal frameshifting and of reactive cysteines involved in S-adenosyl-L-methionine binding."
Eur. J. Biochem. 222:377-386(1994) [PubMed] [Europe PMC] [Abstract]

"Purification and partial sequence analysis of the soluble catechol-O-methyltransferase from human placenta: comparison to the rat liver enzyme."
Biochem. Biophys. Res. Commun. 174:995-1002(1991) [PubMed] [Europe PMC] [Abstract]

"Cell-free synthesis of rat and human catechol O-methyltransferase. Insertion of the membrane-bound form into microsomal membranes in vitro."
Eur. J. Biochem. 202:1013-1020(1991) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Orientation and cellular distribution of membrane-bound catechol-O-methyltransferase in cortical neurons: implications for drug development."
J. Biol. Chem. 286:34752-34760(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Crystal structures of human 108V and 108M catechol O-methyltransferase."
J. Mol. Biol. 380:120-130(2008) [PubMed] [Europe PMC] [Abstract]

"Human catechol-O-methyltransferase pharmacogenetics: description of a functional polymorphism and its potential application to neuropsychiatric disorders."
Pharmacogenetics 6:243-250(1996) [PubMed] [Europe PMC] [Abstract]

"Association between the functional variant of the catechol-O-methyltransferase (COMT) gene and type 1 alcoholism."
Mol. Psychiatry 4:286-289(1999) [PubMed] [Europe PMC] [Abstract]

"Characterization of single-nucleotide polymorphisms in coding regions of human genes."
Nat. Genet. 22:231-238(1999) [PubMed] [Europe PMC] [Abstract]

Erratum
Nat. Genet. 23:373-373(1999)

"Association of Ala72Ser polymorphism with COMT enzyme activity and the risk of schizophrenia in Koreans."
Hum. Genet. 116:319-328(2005) [PubMed] [Europe PMC] [Abstract]

"The V108M mutation decreases the structural stability of catechol O-methyltransferase."
Biochim. Biophys. Acta 1784:1098-1105(2008) [PubMed] [Europe PMC] [Abstract]

"Catechol O-methyltransferase val158-met polymorphism is associated with abdominal obesity and blood pressure in men."
Metabolism 57:708-711(2008) [PubMed] [Europe PMC] [Abstract]