RAB5A, Member RAS Oncogene Family (RAB5A)

RAB5

SPN	N427
HGNC	9783
MGI	105926
PubMed	1999336
RGD	620936
UniProt	P20339
Wiki	RAB5A

The S. cerevisiae YPT1 and SEC4 genes encode Ras-related GTP-binding proteins involved in the regulation of secretion. Mammalian cells express a large number of RAB proteins, GTP-binding proteins closely related to YPT1 and SEC4. Except for the closely related RAB3A and RAB3B, the deduced human RAB proteins share 32 to 50% homology. The predicted 214-amino acid RAB5 protein is 31% and 38% identical to SEC4 and YPT1, respectively. All 6 human RAB proteins tested bound GTP and exhibited GTPase activities in vitro. Northern blot analysis revealed that RAB5 was expressed as 2.7- and 2.8-kb mRNAs in a human fibroblast cell line. RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.

Reference

"The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion."
J. Biol. Chem. 264:12394-12401(1989) [PubMed] [Europe PMC] [Abstract]

"Rab geranylgeranyl transferase catalyzes the geranylgeranylation of adjacent cysteines in the small GTPases Rab1A, Rab3A, and Rab5A."
Proc. Natl. Acad. Sci. U.S.A. 91:11963-11967(1994) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence, annotation and analysis of human chromosome 3."
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Direct interaction of EEA1 with Rab5b."
Eur. J. Biochem. 265:361-366(1999) [PubMed] [Europe PMC] [Abstract]

"A novel membrane-anchored Rab5 interacting protein required for homotypic endosome fusion."
J. Biol. Chem. 275:24661-24669(2000) [PubMed] [Europe PMC] [Abstract]

"Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain."
J. Cell Biol. 151:601-612(2000) [PubMed] [Europe PMC] [Abstract]

"Ras-activated endocytosis is mediated by the Rab5 guanine nucleotide exchange activity of RIN1."
Dev. Cell 1:73-82(2001) [PubMed] [Europe PMC] [Abstract]

"ALS2CL, the novel protein highly homologous to the carboxy-terminal half of ALS2, binds to Rab5 and modulates endosome dynamics."
FEBS Lett. 575:64-70(2004) [PubMed] [Europe PMC] [Abstract]

"Rabip4' is an effector of rab5 and rab4 and regulates transport through early endosomes."
Mol. Biol. Cell 15:611-624(2004) [PubMed] [Europe PMC] [Abstract]

"Regulation of dendritic branching and filopodia formation in hippocampal neurons by specific acylated protein motifs."
Mol. Biol. Cell 15:2205-2217(2004) [PubMed] [Europe PMC] [Abstract]

"Structural basis of family-wide Rab GTPase recognition by rabenosyn-5."
Nature 436:415-419(2005) [PubMed] [Europe PMC] [Abstract]

"Rab5-activating protein 6, a novel endosomal protein with a role in endocytosis."
Biochem. Biophys. Res. Commun. 340:967-975(2006) [PubMed] [Europe PMC] [Abstract]

"Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."
J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Rab1a and Rab5a preferentially bind to binary lipid compositions with higher stored curvature elastic energy."
Mol. Membr. Biol. 30:303-314(2013) [PubMed] [Europe PMC] [Abstract]

"High resolution crystal structures of human Rab5a and five mutants with substitutions in the catalytically important phosphate-binding loop."
J. Biol. Chem. 278:2452-2460(2003) [PubMed] [Europe PMC] [Abstract]

"Refinement of the structure of human Rab5a GTPase domain at 1.05 A resolution."
Acta Crystallogr. D 60:54-60(2004) [PubMed] [Europe PMC] [Abstract]

"Structural basis of Rab5-Rabaptin5 interaction in endocytosis."
Nat. Struct. Mol. Biol. 11:975-983(2004) [PubMed] [Europe PMC] [Abstract]

"Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of early endosomal autoantigen 1 (EEA1)."
Proc. Natl. Acad. Sci. U.S.A. 107:10866-10871(2010) [PubMed] [Europe PMC] [Abstract]