DIX Domain Containing Protein 1 (DIXDC1)

CCD1; Dixin; Coiled-coil protein DIX1; DIX domain-containing protein 1

SPN	M950
HGNC	23695
MGI	2679721
PubMed	22832659
RGD	1309902
UniProt	Q155Q3
Wiki	DIXDC1

The 3-prime UTR of the cDNA contains an Alu element, and the deduced protein contains 493 amino acids. RT-PCR ELISA detected high expression in adult whole brain and in several specific brain regions. Intermediate expression was detected in all other tissues and brain regions examined.

The deduced 472-amino acid DIXDC1 protein has a central myosin tail homology (MTH) region similar to those found in MYH1 and MYH2, as well as a C-terminal DIX domain. Within the MTH region, DIXDC1 contains 2 tyrosine phosphorylation sites and a leucine zipper motif. DIXDC1 was predicted to be a cytoplasmic protein.In contrast with human DIXDC1, the MTH region of mouse Dixdc1 has only 1 tyrosine phosphorylation site and no leucine zipper motif.

Reference

"DIXDC1 isoform, l-DIXDC1, is a novel filamentous actin-binding protein."
Biochem. Biophys. Res. Commun. 347:22-30(2006) [PubMed] [Europe PMC] [Abstract]

"Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
DNA Res. 7:347-355(2000) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]

"The DIX domain protein coiled-coil-DIX1 inhibits c-Jun N-terminal kinase activation by Axin and dishevelled through distinct mechanisms."
J. Biol. Chem. 279:39366-39373(2004) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"DIXDC1 co-localizes and interacts with gamma-tubulin in HEK293 cells."
Cell Biol. Int. 33:697-701(2009) [PubMed] [Europe PMC] [Abstract]

"Wnt signaling stabilizes the DIXDC1 protein through decreased ubiquitin-dependent degradation."
Cancer Sci. 101:700-706(2010) [PubMed] [Europe PMC] [Abstract]

"Strategy for comprehensive identification of human N-myristoylated proteins using an insect cell-free protein synthesis system."
Proteomics 10:1780-1793(2010) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Molecular basis of Wnt activation via the DIX-domain protein Ccd1."
J. Biol. Chem. 286:8597-8608(2011) [PubMed] [Europe PMC] [Abstract]