Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)

4EBP1; BP-1; PHAS-I; Phosphorylated Heat And Acid-Stable Protein Regulated By Insulin 1

SPN	M901
HGNC	3288
MGI	103267
PubMed	23980085
RGD	620259
UniProt	Q13541
Wiki	EIF4EBP1

EIF4EBP1directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.4EBP1 is expressed in most tissues, with highest levels seen in adipose tissue, pancreas, and skeletal muscle. The authors noted that 4EBP1 is more strongly phosphorylated in response to insulin treatment than is 4EBP2, suggesting that 4EBP1 may be more significantly involved in insulin-mediated control pathways.

Reference

"Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function."
Nature 371:762-767(1994) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

Ref.7"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]

"Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E."
EMBO J. 14:5701-5709(1995) [PubMed] [Europe PMC] [Abstract]

"RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1."
Proc. Natl. Acad. Sci. U.S.A. 95:1432-1437(1998) [PubMed] [Europe PMC] [Abstract]

"Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action."
Cell 110:177-189(2002) [PubMed] [Europe PMC] [Abstract]

"TOS motif-mediated raptor binding regulates 4E-BP1 multisite phosphorylation and function."
Curr. Biol. 13:797-806(2003) [PubMed] [Europe PMC] [Abstract]

"The C terminus of initiation factor 4E-binding protein 1 contains multiple regulatory features that influence its function and phosphorylation."
Mol. Cell. Biol. 23:1546-1557(2003) [PubMed] [Europe PMC] [Abstract]

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

"Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."
Electrophoresis 28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]

"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]

"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"Translational homeostasis via the mRNA cap-binding protein, eIF4E."
Mol. Cell 46:847-858(2012) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"The interaction of eIF4E with 4E-BP1 is an induced fit to a completely disordered protein."
Protein Sci. 7:1639-1642(1998) [PubMed] [Europe PMC] [Abstract]

"Structural basis for mRNA cap-binding regulation of eukaryotic initiation factor 4E by 4E-binding protein, studied by spectroscopic, X-ray crystal structural, and molecular dynamics simulation methods."
Biochim. Biophys. Acta 1753:191-208(2005) [PubMed] [Europe PMC] [Abstract]

"Structures of the human eIF4E homologous protein, h4EHP, in its m7GTP-bound and unliganded forms."
J. Mol. Biol. 368:691-705(2007) [PubMed] [Europe PMC] [Abstract]

"Crystallographic and mass spectrometric characterisation of eIF4E with N7-alkylated cap derivatives."
J. Mol. Biol. 372:7-15(2007) [PubMed] [Europe PMC] [Abstract]