Prolactin Induced Protein (PIP)

GCDFP15; GPIP4; BRST2; SABP; gp17; Gross cystic disease fluid protein 15; Prolactin-induced protein; Secretory actin-binding protein

SPN	M363
HGNC	8993
MGI	102696
PubMed	23755096
RGD	70946
UniProt	P12273
Wiki	PIP

The hormonally responsive prolactin-inducible protein gene is expressed in benign and malignant breast tumor tissues and in some normal exocrine organs such as sweat, salivary, and lacrimal glands.The PIP gene is 7 kb long with 4 exons ranging from 106 bp to 223 bp.

PIP is overexpressed in 80% of primary and metastatic breast cancers, is duplicated in the breast carcinoma cell line T47D. The 2 copies are organized as a large palindrome, lying 'in loco' on one chromosome 7. The FRA7I fragile site lies 2 Mb telomeric to the PIP gene and sets the distal end of the repeated sequence. Breakage at the FRA7I site was repaired by 1 cycle of the breakage--fusion-bridge (BFB) cycle mechanism, resulting in amplification of the PIP gene.

Reference

"Isolation and sequencing of a cDNA clone for a prolactin-inducible protein (PIP). Regulation of PIP gene expression in the human breast cancer cell line, T-47D."
J. Biol. Chem. 262:15236-15241(1987) [PubMed] [Europe PMC] [Abstract]

"The prolactin-inducible protein (PIP/GCDFP-15) gene: cloning, structure and regulation."
Mol. Cell. Endocrinol. 80:165-175(1991) [PubMed] [Europe PMC] [Abstract]

"Isolation from a human seminal vesicle library of the cDNA for gp17, a CD4 binding factor."
Immunogenetics 46:345-348(1997) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Human chromosome 7: DNA sequence and biology."
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Primary structure of a new actin-binding protein from human seminal plasma."
Eur. J. Biochem. 196:743-750(1991) [PubMed] [Europe PMC] [Abstract]

"Origin and evolution of gene for prolactin-induced protein."
Gene 383:64-70(2006) [PubMed] [Europe PMC] [Abstract]

"Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry."
J. Proteome Res. 5:1493-1503(2006) [PubMed] [Europe PMC] [Abstract]

"Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry."
Proteomics 8:3833-3847(2008) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma."
J. Mol. Biol. 384:663-672(2008) [PubMed] [Europe PMC] [Abstract]