Serine/Threonine Kinase 39 (STK39)

DCHT; SPAK; STE20/SPS1-related proline-alanine-rich protein kinase

SPN	L129
HGNC	17717
MGI	1858416
PubMed	23527223
RGD	621643
UniProt	Q9UEW8
Wiki	STK39

STK39 encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The deduced 547-amino acid protein has a calculated molecular mass of 59.6 kD. STK39 contains an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detected ubiquitous expression of a 3.8-kb transcript, with most abundant expression in brain and pancreas. Western blot analysis of mouse tissues detected a protein of about 60 kD in all tissues examined.

Reference

"SPAK, a STE20/SPS1-related kinase that activates the p38 pathway."
Oncogene 19:4290-4297(2000) [PubMed] [Europe PMC] [Abstract]

"Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism."
Nat. Commun. 5:3650-3650(2014) [PubMed] [Europe PMC] [Abstract]

"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]

"SPAK kinase is a substrate and target of PKCtheta in T-cell receptor-induced AP-1 activation pathway."
EMBO J. 23:1112-1122(2004) [PubMed] [Europe PMC] [Abstract]

"Toward a global characterization of the phosphoproteome in prostate cancer cells: identification of phosphoproteins in the LNCaP cell line."
Electrophoresis 28:2027-2034(2007) [PubMed] [Europe PMC] [Abstract]

"Phosphoproteome of resting human platelets."
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]