Centromere Protein E (CENPE)
CENP-E; KIF10; Centromere-Associated Protein E
CENP-E is a large protein of ∼312 kDa consisting of an NH2-terminal kinesin motor domain tethered to a globular COOH-terminal domain via an extended “rod” domain predicted to form a coiled coil. Kinesin motors hydrolyze ATP to produce directed mechanical force along microtubules. In the case of CENP-E, the motor activity is involved in establishing and maintaining stable connections between mitotic chromosomes and the microtubules of the mitotic spindle. CENP-E exhibits a periodic accumulation and loss, with maximal levels found during late G2 and M-phases of the cell cycle, and minimum levels in early G1. CENP-E is thus appropriately spatially and temporally positioned to integrate the regulation of the mitotic checkpoint with the mechanics of chromosome-microtubule interactions.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW485Hu01 | Recombinant Centromere Protein E (CENPE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW485Hu01 | Monoclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IP. |
| PAW485Hu01 | Polyclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW485Hu01 | CLIA Kit for Centromere Protein E (CENPE) | CLIA assay for Antigen Detection |
| ELW485Hu01 | ELISA Kit for Centromere Protein E (CENPE) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW485Mu01 | Recombinant Centromere Protein E (CENPE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW485Mu01 | Monoclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IP. |
| PAW485Mu01 | Polyclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW485Mu01 | CLIA Kit for Centromere Protein E (CENPE) | CLIA assay for Antigen Detection |
| ELW485Mu01 | ELISA Kit for Centromere Protein E (CENPE) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW485Ra01 | Recombinant Centromere Protein E (CENPE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW485Ra01 | Monoclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IP. |
| PAW485Ra01 | Polyclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW485Ra01 | CLIA Kit for Centromere Protein E (CENPE) | CLIA assay for Antigen Detection |
| ELW485Ra01 | ELISA Kit for Centromere Protein E (CENPE) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW485Ca01 | Recombinant Centromere Protein E (CENPE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW485Ca01 | Monoclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IP. |
| PAW485Ca01 | Polyclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW485Ca01 | CLIA Kit for Centromere Protein E (CENPE) | CLIA assay for Antigen Detection |
| ELW485Ca01 | ELISA Kit for Centromere Protein E (CENPE) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW485Po01 | Recombinant Centromere Protein E (CENPE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW485Po01 | Monoclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IP. |
| PAW485Po01 | Polyclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW485Po01 | CLIA Kit for Centromere Protein E (CENPE) | CLIA assay for Antigen Detection |
| ELW485Po01 | ELISA Kit for Centromere Protein E (CENPE) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW485Bo01 | Recombinant Centromere Protein E (CENPE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW485Bo01 | Monoclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IP. |
| PAW485Bo01 | Polyclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW485Bo01 | CLIA Kit for Centromere Protein E (CENPE) | CLIA assay for Antigen Detection |
| ELW485Bo01 | ELISA Kit for Centromere Protein E (CENPE) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW485Rb01 | Recombinant Centromere Protein E (CENPE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW485Rb01 | Monoclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IP. |
| PAW485Rb01 | Polyclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW485Rb01 | CLIA Kit for Centromere Protein E (CENPE) | CLIA assay for Antigen Detection |
| ELW485Rb01 | ELISA Kit for Centromere Protein E (CENPE) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW485Mo01 | Recombinant Centromere Protein E (CENPE) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW485Mo01 | Monoclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IP. |
| PAW485Mo01 | Polyclonal Antibody to Centromere Protein E (CENPE) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW485Mo01 | CLIA Kit for Centromere Protein E (CENPE) | CLIA assay for Antigen Detection |
| ELW485Mo01 | ELISA Kit for Centromere Protein E (CENPE) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"CENP-E is a putative kinetochore motor that accumulates just before mitosis."
Nature 359:536-539(1992) [PubMed] [Europe PMC] [Abstract]
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor."
EMBO J. 14:918-926(1995) [PubMed] [Europe PMC] [Abstract]
"Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1."
J. Cell Biol. 143:49-63(1998) [PubMed] [Europe PMC] [Abstract]
"Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules."
J. Biol. Chem. 275:30451-30457(2000) [PubMed] [Europe PMC] [Abstract]
"Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation."
EMBO J. 23:3237-3248(2004) [PubMed] [Europe PMC] [Abstract]
"Human NUF2 interacts with centromere-associated protein E and is essential for a stable spindle microtubule-kinetochore attachment."
J. Biol. Chem. 282:21415-21424(2007) [PubMed] [Europe PMC] [Abstract]
"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry."
Proteomics 7:868-874(2007) [PubMed] [Europe PMC] [Abstract]
"Septin 7 interacts with centromere-associated protein E and is required for its kinetochore localization."
J. Biol. Chem. 283:18916-18925(2008) [PubMed] [Europe PMC] [Abstract]
"SUMO-2/3 modification and binding regulate the association of CENP-E with kinetochores and progression through mitosis."
Mol. Cell 29:729-741(2008) [PubMed] [Europe PMC] [Abstract]
"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
"Defects in chromosome congression and mitotic progression in KIF18A-deficient cells are partly mediated through impaired functions of CENP-E."
Cell Cycle 8:2643-2649(2009) [PubMed] [Europe PMC] [Abstract]
"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
"CENP-E kinesin interacts with SKAP protein to orchestrate accurate chromosome segregation in mitosis."
J. Biol. Chem. 287:1500-1509(2012) [PubMed] [Europe PMC] [Abstract]
"Mutations in CENPE define a novel kinetochore-centromeric mechanism for microcephalic primordial dwarfism."
Hum. Genet. 133:1023-1039(2014) [PubMed] [Europe PMC] [Abstract]
"Crystal structure of the motor domain of the human kinetochore protein CENP-E."
J. Mol. Biol. 340:1107-1116(2004) [PubMed] [Europe PMC] [Abstract]