Protein Kinase, AMP Activated Alpha 1 (PRKAa1)
AMPK; AMPKa1; PKA; ACACA kinase; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-1; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase
5'-AMP-activated protein kinase catalytic subunit alpha-1 is an enzyme belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells.
The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW133Hu01 | Recombinant Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW133Hu01 | Monoclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IP. |
| PAW133Hu01 | Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW133Hu01 | CLIA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | CLIA assay for Antigen Detection |
| ELW133Hu01 | ELISA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW133Mu01 | Recombinant Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW133Mu01 | Monoclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IP. |
| PAW133Mu01 | Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW133Mu01 | CLIA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | CLIA assay for Antigen Detection |
| ELW133Mu01 | ELISA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW133Ra01 | Recombinant Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW133Ra01 | Monoclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IP. |
| PAW133Ra01 | Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW133Ra01 | CLIA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | CLIA assay for Antigen Detection |
| ELW133Ra01 | ELISA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW133Ca01 | Recombinant Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW133Ca01 | Monoclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IP. |
| PAW133Ca01 | Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW133Ca01 | CLIA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | CLIA assay for Antigen Detection |
| ELW133Ca01 | ELISA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW133Po01 | Recombinant Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW133Po01 | Monoclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IP. |
| PAW133Po01 | Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW133Po01 | CLIA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | CLIA assay for Antigen Detection |
| ELW133Po01 | ELISA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW133Bo01 | Recombinant Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW133Bo01 | Monoclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IP. |
| PAW133Bo01 | Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW133Bo01 | CLIA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | CLIA assay for Antigen Detection |
| ELW133Bo01 | ELISA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW133Rb01 | Recombinant Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW133Rb01 | Monoclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IP. |
| PAW133Rb01 | Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW133Rb01 | CLIA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | CLIA assay for Antigen Detection |
| ELW133Rb01 | ELISA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW133Mo01 | Recombinant Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW133Mo01 | Monoclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IP. |
| PAW133Mo01 | Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW133Mo01 | CLIA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | CLIA assay for Antigen Detection |
| ELW133Mo01 | ELISA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"The DNA sequence and comparative analysis of human chromosome 5."
Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
"Mammalian AMP-activated protein kinase subfamily."
J. Biol. Chem. 271:611-614(1996) [PubMed] [Europe PMC] [Abstract]
"Functional domains of the alpha1 catalytic subunit of the AMP-activated protein kinase."
J. Biol. Chem. 273:35347-35354(1998) [PubMed] [Europe PMC] [Abstract]
"Cell cycle regulation via p53 phosphorylation by a 5'-AMP activated protein kinase activator, 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside, in a human hepatocellular carcinoma cell line."
Biochem. Biophys. Res. Commun. 287:562-567(2001) [PubMed] [Europe PMC] [Abstract]
"Regulation of transcription by AMP-activated protein kinase: phosphorylation of p300 blocks its interaction with nuclear receptors."
J. Biol. Chem. 276:38341-38344(2001) [PubMed] [Europe PMC] [Abstract]
"Role of AMP-activated protein kinase in mechanism of metformin action."
J. Clin. Invest. 108:1167-1174(2001) [PubMed] [Europe PMC] [Abstract]
"Physiological modulation of CFTR activity by AMP-activated protein kinase in polarized T84 cells."
Am. J. Physiol. 284:C1297-C1308(2003) [PubMed] [Europe PMC] [Abstract]
"TSC2 mediates cellular energy response to control cell growth and survival."
Cell 115:577-590(2003) [PubMed] [Europe PMC] [Abstract]
"LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1."
EMBO J. 23:833-843(2004) [PubMed] [Europe PMC] [Abstract]
"Calmodulin-dependent protein kinase kinase-beta is an alternative upstream kinase for AMP-activated protein kinase."
Cell Metab. 2:9-19(2005) [PubMed] [Europe PMC] [Abstract]
"The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases."
J. Biol. Chem. 280:29060-29066(2005) [PubMed] [Europe PMC] [Abstract]
"AMP-activated protein kinase induces a p53-dependent metabolic checkpoint."
Mol. Cell 18:283-293(2005) [PubMed] [Europe PMC] [Abstract]
"Folliculin encoded by the BHD gene interacts with a binding protein, FNIP1, and AMPK, and is involved in AMPK and mTOR signaling."
Proc. Natl. Acad. Sci. U.S.A. 103:15552-15557(2006) [PubMed] [Europe PMC] [Abstract]
"Conserved alpha-helix acts as autoinhibitory sequence in AMP-activated protein kinase alpha subunits."
J. Biol. Chem. 282:495-506(2007) [PubMed] [Europe PMC] [Abstract]
"The energy sensor AMP-activated protein kinase directly regulates the mammalian FOXO3 transcription factor."
J. Biol. Chem. 282:30107-30119(2007) [PubMed] [Europe PMC] [Abstract]
"Energy-dependent regulation of cell structure by AMP-activated protein kinase."
Nature 447:1017-1020(2007) [PubMed] [Europe PMC] [Abstract]
"AMP-activated protein kinase regulates GLUT4 transcription by phosphorylating histone deacetylase 5."
Diabetes 57:860-867(2008) [PubMed] [Europe PMC] [Abstract]
"Identification and characterization of a novel folliculin-interacting protein FNIP2."
Gene 415:60-67(2008) [PubMed] [Europe PMC] [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
"Phosphoproteome of resting human platelets."
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
"AMPK phosphorylation of raptor mediates a metabolic checkpoint."
Mol. Cell 30:214-226(2008) [PubMed] [Europe PMC] [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
"Interaction of folliculin (Birt-Hogg-Dube gene product) with a novel Fnip1-like (FnipL/Fnip2) protein."
Oncogene 27:5339-5347(2008) [PubMed] [Europe PMC] [Abstract]
"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
"Large-scale proteomics analysis of the human kinome."
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
"Cell-wide analysis of secretory granule dynamics in three dimensions in living pancreatic beta-cells: evidence against a role for AMPK-dependent phosphorylation of KLC1 at Ser517/Ser520 in glucose-stimulated insulin granule movement."
Biochem. Soc. Trans. 38:205-208(2010) [PubMed] [Europe PMC] [Abstract]
"ATM signals to TSC2 in the cytoplasm to regulate mTORC1 in response to ROS."
Proc. Natl. Acad. Sci. U.S.A. 107:4153-4158(2010) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
"Ulk1-mediated phosphorylation of AMPK constitutes a negative regulatory feedback loop."
Autophagy 7:696-706(2011) [PubMed] [Europe PMC] [Abstract]
"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
"Phosphorylation of ULK1 (hATG1) by AMP-activated protein kinase connects energy sensing to mitophagy."
Science 331:456-461(2011) [PubMed] [Europe PMC] [Abstract]
"AMPK is a direct adenylate charge-regulated protein kinase."
Science 332:1433-1435(2011) [PubMed] [Europe PMC] [Abstract]
"AMP-activated protein kinase in metabolic control and insulin signaling."
Circ. Res. 100:328-341(2007) [PubMed] [Europe PMC] [Abstract]
"AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy."
Nat. Rev. Mol. Cell Biol. 8:774-785(2007) [PubMed] [Europe PMC] [Abstract]
"N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells."
Biochem. J. 449:741-749(2013) [PubMed] [Europe PMC] [Abstract]
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
"The consensus coding sequences of human breast and colorectal cancers."
Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]