Caspase 8 (CASP8)
CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog
Caspase 8 is a member caspase family. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV958Hu01 | Recombinant Caspase 8 (CASP8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV958Hu01 | Monoclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IP. |
| PAV958Hu01 | Polyclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV958Hu01 | CLIA Kit for Caspase 8 (CASP8) | CLIA assay for Antigen Detection |
| ELV958Hu01 | ELISA Kit for Caspase 8 (CASP8) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV958Mu01 | Recombinant Caspase 8 (CASP8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV958Mu01 | Monoclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IP. |
| PAV958Mu01 | Polyclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV958Mu01 | CLIA Kit for Caspase 8 (CASP8) | CLIA assay for Antigen Detection |
| ELV958Mu01 | ELISA Kit for Caspase 8 (CASP8) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV958Ra01 | Recombinant Caspase 8 (CASP8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV958Ra01 | Monoclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IP. |
| PAV958Ra01 | Polyclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV958Ra01 | CLIA Kit for Caspase 8 (CASP8) | CLIA assay for Antigen Detection |
| ELV958Ra01 | ELISA Kit for Caspase 8 (CASP8) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV958Ca01 | Recombinant Caspase 8 (CASP8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV958Ca01 | Monoclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IP. |
| PAV958Ca01 | Polyclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV958Ca01 | CLIA Kit for Caspase 8 (CASP8) | CLIA assay for Antigen Detection |
| ELV958Ca01 | ELISA Kit for Caspase 8 (CASP8) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV958Po01 | Recombinant Caspase 8 (CASP8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV958Po01 | Monoclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IP. |
| PAV958Po01 | Polyclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV958Po01 | CLIA Kit for Caspase 8 (CASP8) | CLIA assay for Antigen Detection |
| ELV958Po01 | ELISA Kit for Caspase 8 (CASP8) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV958Bo01 | Recombinant Caspase 8 (CASP8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV958Bo01 | Monoclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IP. |
| PAV958Bo01 | Polyclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV958Bo01 | CLIA Kit for Caspase 8 (CASP8) | CLIA assay for Antigen Detection |
| ELV958Bo01 | ELISA Kit for Caspase 8 (CASP8) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV958Rb01 | Recombinant Caspase 8 (CASP8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV958Rb01 | Monoclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IP. |
| PAV958Rb01 | Polyclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV958Rb01 | CLIA Kit for Caspase 8 (CASP8) | CLIA assay for Antigen Detection |
| ELV958Rb01 | ELISA Kit for Caspase 8 (CASP8) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV958Mo01 | Recombinant Caspase 8 (CASP8) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV958Mo01 | Monoclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IP. |
| PAV958Mo01 | Polyclonal Antibody to Caspase 8 (CASP8) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV958Mo01 | CLIA Kit for Caspase 8 (CASP8) | CLIA assay for Antigen Detection |
| ELV958Mo01 | ELISA Kit for Caspase 8 (CASP8) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death."
Cell 85:803-815(1996) [PubMed] [Europe PMC] [Abstract]
"FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex."
Cell 85:817-827(1996) [PubMed] [Europe PMC] [Abstract]
"In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains."
Proc. Natl. Acad. Sci. U.S.A. 93:7464-7469(1996) [PubMed] [Europe PMC] [Abstract]
"FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis."
J. Biol. Chem. 272:18542-18545(1997) [PubMed] [Europe PMC] [Abstract]
"Structure and chromosome localization of the human CASP8 gene."
Gene 226:225-232(1999) [PubMed] [Europe PMC] [Abstract]
"Cloning and characterization of three novel genes, ALS2CR1, ALS2CR2, and ALS2CR3, in the juvenile amyotrophic lateral sclerosis (ALS2) critical region at chromosome 2q33-q34: candidate genes for ALS2."
Genomics 71:200-213(2001) [PubMed] [Europe PMC] [Abstract]
"Characterization of caspase-8L: a novel isoform of caspase-8 that behaves as an inhibitor of the caspase cascade."
Blood 99:4070-4078(2002) [PubMed] [Europe PMC] [Abstract]
"The procaspase-8 isoform, procaspase-8L, recruited to the BAP31 complex at the endoplasmic reticulum."
Proc. Natl. Acad. Sci. U.S.A. 99:4331-4336(2002) [PubMed] [Europe PMC] [Abstract]
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases."
Proc. Natl. Acad. Sci. U.S.A. 93:14486-14491(1996) [PubMed] [Europe PMC] [Abstract]
"FLICE induced apoptosis in a cell-free system. Cleavage of caspase zymogens."
J. Biol. Chem. 272:2952-2956(1997) [PubMed] [Europe PMC] [Abstract]
"FLICE is activated by association with the CD95 death-inducing signaling complex (DISC)."
EMBO J. 16:2794-2804(1997) [PubMed] [Europe PMC] [Abstract]
"Dominant expression of a novel splice variant of caspase-8 in human peripheral blood lymphocytes."
Biochem. Biophys. Res. Commun. 272:877-881(2000) [PubMed] [Europe PMC] [Abstract]
"p28 Bap31, a Bcl-2/Bcl-XL- and procaspase-8-associated protein in the endoplasmic reticulum."
J. Cell Biol. 139:327-338(1997) [PubMed] [Europe PMC] [Abstract]
"PED/PEA-15: an anti-apoptotic molecule that regulates FAS/TNFR1-induced apoptosis."
Oncogene 18:4409-4415(1999) [PubMed] [Europe PMC] [Abstract]
"A cytomegalovirus-encoded inhibitor of apoptosis that suppresses caspase-8 activation."
Proc. Natl. Acad. Sci. U.S.A. 98:7829-7834(2001) [PubMed] [Europe PMC] [Abstract]
"An unappreciated role for RNA surveillance."
Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract]
"Calcium binding of ARC mediates regulation of caspase 8 and cell death."
Mol. Cell. Biol. 24:9763-9770(2004) [PubMed] [Europe PMC] [Abstract]
"Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth."
Proc. Natl. Acad. Sci. U.S.A. 101:6170-6175(2004) [PubMed] [Europe PMC] [Abstract]
"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
"Role of FLASH in caspase-8-mediated activation of NF-kappaB: dominant-negative function of FLASH mutant in NF-kappaB signaling pathway."
Oncogene 24:688-696(2005) [PubMed] [Europe PMC] [Abstract]
"FLASH links the CD95 signaling pathway to the cell nucleus and nuclear bodies."
EMBO J. 26:391-401(2007) [PubMed] [Europe PMC] [Abstract]
"Cdk1/cyclin B1 controls Fas-mediated apoptosis by regulating caspase-8 activity."
Mol. Cell. Biol. 30:5726-5740(2010) [PubMed] [Europe PMC] [Abstract]
"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
"The E. coli effector protein NleF is a caspase inhibitor."
PLoS ONE 8:E58937-E58937(2013) [PubMed] [Europe PMC] [Abstract]
"The three-dimensional structure of caspase-8: an initiator enzyme in apoptosis."
Structure 7:1125-1133(1999) [PubMed] [Europe PMC] [Abstract]
"The atomic-resolution structure of human caspase-8, a key activator of apoptosis."
Structure 7:1135-1143(1999) [PubMed] [Europe PMC] [Abstract]
"Pleiotropic defects in lymphocyte activation caused by caspase-8 mutations lead to human immunodeficiency."
Nature 419:395-399(2002) [PubMed] [Europe PMC] [Abstract]
"Association of a common variant of the CASP8 gene with reduced risk of breast cancer."
J. Natl. Cancer Inst. 96:1866-1869(2004) [PubMed] [Europe PMC] [Abstract]
"A common coding variant in CASP8 is associated with breast cancer risk."
Nat. Genet. 39:352-358(2007) [PubMed] [Europe PMC] [Abstract]
Erratum
Nat. Genet. 39:688-688(2007)
"A six-nucleotide insertion-deletion polymorphism in the CASP8 promoter is associated with susceptibility to multiple cancers."
Nat. Genet. 39:605-613(2007) [PubMed] [Europe PMC] [Abstract]
"Genetic variants and haplotypes of the caspase-8 and caspase-10 genes contribute to susceptibility to cutaneous melanoma."
Hum. Mutat. 29:1443-1451(2008) [PubMed] [Europe PMC] [Abstract]