Call Now

Myosin Light Chain Kinase (MYLK)

KRP; MLCK108; MLCK210; MSTP083; MLCK; smMLCK; MYLK1; MLCK1; Telokin; Myosin Light Chain Kinase, Smooth Muscle, deglutamylated form; Kinase-related protein

Myosin light chain kinase, smooth muscle is a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity.

This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3.

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV707Hu01 Recombinant Myosin Light Chain Kinase (MYLK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV707Hu01 Monoclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IP.
PAV707Hu01 Polyclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IF.
Assay Kits CLV707Hu01 CLIA Kit for Myosin Light Chain Kinase (MYLK) CLIA assay for Antigen Detection
ELV707Hu01 ELISA Kit for Myosin Light Chain Kinase (MYLK) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV707Mu01 Recombinant Myosin Light Chain Kinase (MYLK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV707Mu01 Monoclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IP.
PAV707Mu01 Polyclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IF.
Assay Kits CLV707Mu01 CLIA Kit for Myosin Light Chain Kinase (MYLK) CLIA assay for Antigen Detection
ELV707Mu01 ELISA Kit for Myosin Light Chain Kinase (MYLK) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV707Ra01 Recombinant Myosin Light Chain Kinase (MYLK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV707Ra01 Monoclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IP.
PAV707Ra01 Polyclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IF.
Assay Kits CLV707Ra01 CLIA Kit for Myosin Light Chain Kinase (MYLK) CLIA assay for Antigen Detection
ELV707Ra01 ELISA Kit for Myosin Light Chain Kinase (MYLK) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV707Ca01 Recombinant Myosin Light Chain Kinase (MYLK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV707Ca01 Monoclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IP.
PAV707Ca01 Polyclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IF.
Assay Kits CLV707Ca01 CLIA Kit for Myosin Light Chain Kinase (MYLK) CLIA assay for Antigen Detection
ELV707Ca01 ELISA Kit for Myosin Light Chain Kinase (MYLK) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV707Po01 Recombinant Myosin Light Chain Kinase (MYLK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV707Po01 Monoclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IP.
PAV707Po01 Polyclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IF.
Assay Kits CLV707Po01 CLIA Kit for Myosin Light Chain Kinase (MYLK) CLIA assay for Antigen Detection
ELV707Po01 ELISA Kit for Myosin Light Chain Kinase (MYLK) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV707Bo01 Recombinant Myosin Light Chain Kinase (MYLK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV707Bo01 Monoclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IP.
PAV707Bo01 Polyclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IF.
Assay Kits CLV707Bo01 CLIA Kit for Myosin Light Chain Kinase (MYLK) CLIA assay for Antigen Detection
ELV707Bo01 ELISA Kit for Myosin Light Chain Kinase (MYLK) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV707Rb01 Recombinant Myosin Light Chain Kinase (MYLK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV707Rb01 Monoclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IP.
PAV707Rb01 Polyclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IF.
Assay Kits CLV707Rb01 CLIA Kit for Myosin Light Chain Kinase (MYLK) CLIA assay for Antigen Detection
ELV707Rb01 ELISA Kit for Myosin Light Chain Kinase (MYLK) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV707Mo01 Recombinant Myosin Light Chain Kinase (MYLK) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV707Mo01 Monoclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IP.
PAV707Mo01 Polyclonal Antibody to Myosin Light Chain Kinase (MYLK) WB; IHC; ICC; IF.
Assay Kits CLV707Mo01 CLIA Kit for Myosin Light Chain Kinase (MYLK) CLIA assay for Antigen Detection
ELV707Mo01 ELISA Kit for Myosin Light Chain Kinase (MYLK) Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"The human myosin light chain kinase (MLCK) from hippocampus: cloning, sequencing, expression, and localization to 3qcen-q21."
Genomics 29:562-570(1995) [PubMed] [Europe PMC] [Abstract]

"Myosin light chain kinase in endothelium: molecular cloning and regulation."
Am. J. Respir. Cell Mol. Biol. 16:489-494(1997) [PubMed] [Europe PMC] [Abstract]

"A single human myosin light chain kinase gene (MLCK; MYLK)."
Genomics 57:256-267(1999) [PubMed] [Europe PMC] [Abstract]

"Differential regulation of alternatively spliced endothelial cell myosin light chain kinase isoforms by p60(Src)."
J. Biol. Chem. 276:8567-8573(2001) [PubMed] [Europe PMC] [Abstract]

"Analysis of the kinase-related protein gene found at human chromosome 3q21 in a multi-gene cluster: organization, expression, alternative splicing and polymorphic marker."
J. Cell. Biochem. 75:481-491(1999) [PubMed] [Europe PMC] [Abstract]

"A differentiation-dependent splice variant of myosin light chain kinase, MLCK1, regulates epithelial tight junction permeability."
J. Biol. Chem. 279:55506-55513(2004) [PubMed] [Europe PMC] [Abstract]

"The N-terminus of the long MLCK induces a disruption in normal spindle morphology and metaphase arrest."
J. Cell Sci. 117:1481-1493(2004) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence, annotation and analysis of human chromosome 3."
Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Novel interaction of cortactin with endothelial cell myosin light chain kinase."
Biochem. Biophys. Res. Commun. 298:511-519(2002) [PubMed] [Europe PMC] [Abstract]

"Myosin light chain kinase modulates hypotonicity-induced Ca2+ entry and Cl- channel activity in human cervical cancer cells."
Pflugers Arch. 444:276-285(2002) [PubMed] [Europe PMC] [Abstract]

"Quantitative measurements of Ca(2+)/calmodulin binding and activation of myosin light chain kinase in cells."
FEBS Lett. 557:121-124(2004) [PubMed] [Europe PMC] [Abstract]

"Distinct temporal-spatial roles for rho kinase and myosin light chain kinase in epithelial purse-string wound closure."
Gastroenterology 128:987-1001(2005) [PubMed] [Europe PMC] [Abstract]

"Tumor necrosis factor-induced long myosin light chain kinase transcription is regulated by differentiation-dependent signaling events. Characterization of the human long myosin light chain kinase promoter."
J. Biol. Chem. 281:26205-26215(2006) [PubMed] [Europe PMC] [Abstract]

"Myosin light chain kinase plays a role in the regulation of epithelial cell survival."
J. Cell Sci. 119:2269-2281(2006) [PubMed] [Europe PMC] [Abstract]

"Ca2+-calmodulin-dependent myosin light chain kinase is essential for activation of TRPC5 channels expressed in HEK293 cells."
J. Physiol. (Lond.) 570:219-235(2006) [PubMed] [Europe PMC] [Abstract]

"Myosin light-chain kinase contributes to the proliferation and migration of breast cancer cells through cross-talk with activated ERK1/2."
Cancer Lett. 270:312-327(2008) [PubMed] [Europe PMC] [Abstract]

"Phosphoproteome of resting human platelets."
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]

"Nonmuscle myosin light-chain kinase mediates neutrophil transmigration in sepsis-induced lung inflammation by activating beta2 integrins."
Nat. Immunol. 9:880-886(2008) [PubMed] [Europe PMC] [Abstract]

"Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Androgens down-regulate myosin light chain kinase in human prostate cancer cells."
J. Steroid Biochem. Mol. Biol. 114:174-179(2009) [PubMed] [Europe PMC] [Abstract]

"Arrest defective-1 controls tumor cell behavior by acetylating myosin light chain kinase."
PLoS ONE 4:E7451-E7451(2009) [PubMed] [Europe PMC] [Abstract]

"Myosin light chain kinase is responsible for high proliferative ability of breast cancer cells via anti-apoptosis involving p38 pathway."
Acta Pharmacol. Sin. 31:725-732(2010) [PubMed] [Europe PMC] [Abstract]

"Modulation of factors affecting optic nerve head astrocyte migration."
Invest. Ophthalmol. Vis. Sci. 51:4096-4103(2010) [PubMed] [Europe PMC] [Abstract]

"Quantitative distribution and colocalization of non-muscle myosin light chain kinase isoforms and cortactin in human lung endothelium."
Microvasc. Res. 80:75-88(2010) [PubMed] [Europe PMC] [Abstract]

"Abl tyrosine kinase phosphorylates nonmuscle Myosin light chain kinase to regulate endothelial barrier function."
Mol. Biol. Cell 21:4042-4056(2010) [PubMed] [Europe PMC] [Abstract]

"The angiotensin II type 1 receptor induces membrane blebbing by coupling to Rho A, Rho kinase, and myosin light chain kinase."
Mol. Pharmacol. 77:903-911(2010) [PubMed] [Europe PMC] [Abstract]

"Non-muscle myosin light chain kinase isoform is a viable molecular target in acute inflammatory lung injury."
Am. J. Respir. Cell Mol. Biol. 44:40-52(2011) [PubMed] [Europe PMC] [Abstract]

"Organometallic pyridylnaphthalimide complexes as protein kinase inhibitors."
Organometallics 30:4598-4606(2011) [PubMed] [Europe PMC] [Abstract]

"Myosin, transgelin, and myosin light chain kinase: expression and function in asthma."
Am. J. Respir. Crit. Care Med. 179:194-204(2009) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Solution structure of the eighth Ig-like domain of human myosin light chain kinase."
Submitted (NOV-2005) to the PDB data bank

"A coupled equilibrium shift mechanism in calmodulin-mediated signal transduction."
Structure 16:736-746(2008) [PubMed] [Europe PMC] [Abstract]

"Patterns of somatic mutation in human cancer genomes."
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]

"Mutations in myosin light chain kinase cause familial aortic dissections."
Am. J. Hum. Genet. 87:701-707(2010) [PubMed] [Europe PMC] [Abstract]