Integrin Beta 3 (ITGb3)

CD61; ITG-B3; GP3A; GP3-A; GpIIIa; Platelet Membrane Glycoprotein IIIa

SPN	V565
HGNC	6156
MGI	96612
PubMed	15292919
RGD	628868
UniProt	P05106
Wiki	CD61

CD61 is a cluster of differentiation found on thrombocytes. The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.

The platelet and endothelial cell heterodimers use GP IIIa as the beta subunit; the leukocyte heterodimers contain a beta subunit with a molecular mass of 95 kD that is homologous to GP IIIa but is clearly a different protein; and the fibronectin receptors contain a beta subunit that appears to be analogous to band 3 of integrin.

Reference

"Protein sequence of endothelial glycoprotein IIIa derived from a cDNA clone. Identity with platelet glycoprotein IIIa and similarity to 'integrin'."
J. Biol. Chem. 262:3936-3939(1987) [PubMed] [Europe PMC] [Abstract]

"Structure of platelet glycoprotein IIIa. A common subunit for two different membrane receptors."
J. Clin. Invest. 81:1470-1475(1988) [PubMed] [Europe PMC] [Abstract]

"GPIIb and GPIIIa amino acid sequences deduced from human megakaryocyte cDNAs."
Mol. Biol. Rep. 14:27-33(1990) [PubMed] [Europe PMC] [Abstract]

"Cloning and characterization of a novel integrin beta3 subunit."
J. Biol. Chem. 272:16390-16397(1997) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Isolation and characterization of a TATA-less promoter for the human beta 3 integrin gene."
Blood 83:668-676(1994) [PubMed] [Europe PMC] [Abstract]

"Cloning of glycoprotein IIIa cDNA from human erythroleukemia cells and localization of the gene to chromosome 17."
Blood 72:593-600(1988) [PubMed] [Europe PMC] [Abstract]

"Separation of important new platelet glycoproteins (GPIa, GPIc, GPIc*, GPIIa and GMP-140) by F.P.L.C. Characterization by monoclonal antibodies and gas-phase sequencing."
Biochem. J. 279:419-425(1991) [PubMed] [Europe PMC] [Abstract]

"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]

"The genomic organization of platelet glycoprotein IIIa."
J. Biol. Chem. 265:8590-8595(1990) [PubMed] [Europe PMC] [Abstract]

"Characterization of the human platelet glycoprotein IIIa gene. Comparison with the fibronectin receptor beta-subunit gene."
J. Biol. Chem. 265:18098-18103(1990) [PubMed] [Europe PMC] [Abstract]

"The gene organization of the human beta 7 subunit, the common beta subunit of the leukocyte integrins HML-1 and LPAM-1."
Int. Immunol. 4:1031-1040(1992) [PubMed] [Europe PMC] [Abstract]

"Purification and partial amino acid sequence of human platelet membrane glycoproteins IIb and IIIa."
Blood 69:560-564(1987) [PubMed] [Europe PMC] [Abstract]

"An alternative cytoplasmic domain of the integrin beta 3 subunit."
Proc. Natl. Acad. Sci. U.S.A. 86:5415-5418(1989) [PubMed] [Europe PMC] [Abstract]

"Assignment of disulphide bonds in human platelet GPIIIa. A disulphide pattern for the beta-subunits of the integrin family."
Biochem. J. 274:63-71(1991) [PubMed] [Europe PMC] [Abstract]

"Outside-in integrin signal transduction. Alpha IIb beta 3-(GP IIb IIIa) tyrosine phosphorylation induced by platelet aggregation."
J. Biol. Chem. 271:10811-10815(1996) [PubMed] [Europe PMC] [Abstract]

"The Tat protein of human immunodeficiency virus type-1 promotes vascular cell growth and locomotion by engaging the alpha5beta1 and alphavbeta3 integrins and by mobilizing sequestered basic fibroblast growth factor."
Blood 94:663-672(1999) [PubMed] [Europe PMC] [Abstract]

"Threonine phosphorylation of the beta 3 integrin cytoplasmic tail, at a site recognized by PDK1 and Akt/PKB in vitro, regulates Shc binding."
J. Biol. Chem. 275:30901-30906(2000) [PubMed] [Europe PMC] [Abstract]

"Coordinate interactions of Csk, Src, and Syk kinases with [alpha]IIb[beta]3 initiate integrin signaling to the cytoskeleton."
J. Cell Biol. 157:265-275(2002) [PubMed] [Europe PMC] [Abstract]

"Different splice variants of filamin-B affect myogenesis, subcellular distribution, and determine binding to integrin (beta) subunits."
J. Cell Biol. 156:361-376(2002) [PubMed] [Europe PMC] [Abstract]

"Myosin-X provides a motor-based link between integrins and the cytoskeleton."
Nat. Cell Biol. 6:523-531(2004) [PubMed] [Europe PMC] [Abstract]

"A role for the thiol isomerase protein ERP5 in platelet function."
Blood 105:1500-1507(2005) [PubMed] [Europe PMC] [Abstract]

"Epithelial membrane protein-2 regulates surface expression of alphavbeta3 integrin in the endometrium."
Dev. Biol. 287:336-345(2005) [PubMed] [Europe PMC] [Abstract]

"Cartilage oligomeric matrix protein/thrombospondin 5 supports chondrocyte attachment through interaction with integrins."
J. Biol. Chem. 280:32655-32661(2005) [PubMed] [Europe PMC] [Abstract]

"Structure and function of the platelet integrin alphaIIbbeta3."
J. Clin. Invest. 115:3363-3369(2005) [PubMed] [Europe PMC] [Abstract]

"Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry."
J. Proteome Res. 4:2070-2080(2005) [PubMed] [Europe PMC] [Abstract]

"Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach."
Mol. Cell. Proteomics 5:226-233(2006) [PubMed] [Europe PMC] [Abstract]

"Platelet integrin alpha(IIb)beta(3): activation mechanisms."
J. Thromb. Haemost. 5:1345-1352(2007) [PubMed] [Europe PMC] [Abstract]

"Phosphoproteome of resting human platelets."
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]

"Integrin alphaVbeta3 Binds to the RGD motif of glycoprotein B of Kaposi's sarcoma-associated herpesvirus and functions as an RGD-dependent entry receptor."
J. Virol. 82:1570-1580(2008) [PubMed] [Europe PMC] [Abstract]

"Naturally processed peptides spanning the HPA-1a polymorphism are efficiently generated and displayed from platelet glycoprotein by HLA-DRB3*0101-positive antigen-presenting cells."
Blood 114:1954-1957(2009) [PubMed] [Europe PMC] [Abstract]

"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]

"Tyrosine phosphorylation of integrin beta3 regulates kindlin-2 binding and integrin activation."
J. Biol. Chem. 285:30370-30374(2010) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of the extracellular segment of integrin alpha Vbeta3."
Science 294:339-345(2001) [PubMed] [Europe PMC] [Abstract]

"Crystallographic structure of the human leukocyte antigen DRA, DRB3*0101: models of a directional alloimmune response and autoimmunity."
J. Mol. Biol. 371:435-446(2007) [PubMed] [Europe PMC] [Abstract]

"Inherited diseases of platelet glycoproteins: considerations for rapid molecular characterization."
Thromb. Haemost. 72:492-502(1994) [PubMed] [Europe PMC] [Abstract]

"The human platelet alloantigens, PlA1 and PlA2, are associated with a leucine33/proline33 amino acid polymorphism in membrane glycoprotein IIIa, and are distinguishable by DNA typing."
J. Clin. Invest. 83:1778-1781(1989) [PubMed] [Europe PMC] [Abstract]

"An amino acid polymorphism within the RGD binding domain of platelet membrane glycoprotein IIIa is responsible for the formation of the Pena/Penb alloantigen system."
J. Clin. Invest. 90:2038-2043(1992) [PubMed] [Europe PMC] [Abstract]

"Single point mutation in human glycoprotein IIIa is associated with a new platelet-specific alloantigen (Mo) involved in neonatal alloimmune thrombocytopenia."
Blood 81:70-76(1993) [PubMed] [Europe PMC] [Abstract]

"Amino acid 489 is encoded by a mutational 'hot spot' on the beta 3 integrin chain: the CA/TU human platelet alloantigen system."
Blood 82:3386-3391(1993) [PubMed] [Europe PMC] [Abstract]

"A point mutation leads to an unpaired cysteine residue and a molecular weight polymorphism of a functional platelet beta 3 integrin subunit. The Sra alloantigen system of GPIIIa."
J. Biol. Chem. 269:8439-8444(1994) [PubMed] [Europe PMC] [Abstract]

"A beta 3 integrin mutation abolishes ligand binding and alters divalent cation-dependent conformation."
Science 249:915-918(1990) [PubMed] [Europe PMC] [Abstract]

"A spontaneous mutation of integrin alpha IIb beta 3 (platelet glycoprotein IIb-IIIa) helps define a ligand binding site."
J. Biol. Chem. 267:3789-3794(1992) [PubMed] [Europe PMC] [Abstract]

"A new variant of Glanzmann's thrombasthenia (Strasbourg I). Platelets with functionally defective glycoprotein IIb-IIIa complexes and a glycoprotein IIIa 214Arg-->214Trp mutation."
J. Clin. Invest. 89:1995-2004(1992) [PubMed] [Europe PMC] [Abstract]

"Ser-752-->Pro mutation in the cytoplasmic domain of integrin beta 3 subunit and defective activation of platelet integrin alpha IIb beta 3 (glycoprotein IIb-IIIa) in a variant of Glanzmann thrombasthenia."
Proc. Natl. Acad. Sci. U.S.A. 89:10169-10173(1992) [PubMed] [Europe PMC] [Abstract]

"A Cys374Tyr homozygous mutation of platelet glycoprotein IIIa (beta 3) in a Chinese patient with Glanzmann's thrombasthenia."
Blood 88:1666-1675(1996) [PubMed] [Europe PMC] [Abstract]

"A Leu117-->Trp mutation within the RGD-peptide cross-linking region of beta3 results in Glanzmann thrombasthenia by preventing alphaIIb beta3 export to the platelet surface."
Blood 90:3082-3088(1997) [PubMed] [Europe PMC] [Abstract]

"Hematologically important mutations: Glanzmann thrombasthenia."
Blood Cells Mol. Dis. 23:39-51(1997) [PubMed] [Europe PMC] [Abstract]

"Three novel integrin beta3 subunit missense mutations (H280P, C560F, and G579S) in thrombasthenia, including one (H280P) prevalent in Japanese patients."
Biochem. Biophys. Res. Commun. 251:763-768(1998) [PubMed] [Europe PMC] [Abstract]

"A Ser162-->Leu mutation within glycoprotein (GP) IIIa (integrin beta3) results in an unstable alphaIIbbeta3 complex that retains partial function in a novel form of type II Glanzmann thrombasthenia."
Thromb. Haemost. 80:42-48(1998) [PubMed] [Europe PMC] [Abstract]

"Homozygous Cys542-->Arg substitution in GPIIIa in a Swiss patient with type I Glanzmann's thrombasthenia."
Br. J. Haematol. 105:523-531(1999) [PubMed] [Europe PMC] [Abstract]

"Characterization of single-nucleotide polymorphisms in coding regions of human genes."
Nat. Genet. 22:231-238(1999) [PubMed] [Europe PMC] [Abstract]

Erratum
Nat. Genet. 23:373-373(1999)

"A point mutation in the cysteine-rich domain of glycoprotein (GP) IIIa results in the expression of a GPIIb-IIIa (alphaIIbbeta3) integrin receptor locked in a high-affinity state and a Glanzmann thrombasthenia-like phenotype."
Blood 98:2432-2441(2001) [PubMed] [Europe PMC] [Abstract]

"A new platelet polymorphism Duv(a+), localized within the RGD binding domain of glycoprotein IIIa, is associated with neonatal thrombocytopenia."
Blood 99:4449-4456(2002) [PubMed] [Europe PMC] [Abstract]

"A novel 196Leu to Pro substitution in the beta3 subunit of the alphaIIbbeta3 integrin in a patient with a variant form of Glanzmann thrombasthenia."
Platelets 13:101-111(2002) [PubMed] [Europe PMC] [Abstract]

"Glanzmann's thrombasthenia: identification of 19 new mutations in 30 patients."
Thromb. Haemost. 87:1034-1042(2002) [PubMed] [Europe PMC] [Abstract]

"Two new beta3 integrin mutations in Indian patients with Glanzmann thrombasthenia: localization of mutations affecting cysteine residues in integrin beta3."
Thromb. Haemost. 88:503-509(2002) [PubMed] [Europe PMC] [Abstract]

"A variant thrombasthenic phenotype associated with compound heterozygosity of integrin beta3-subunit: (Met124Val)beta3 alters the subunit dimerization rendering a decreased number of constitutive active alphaIIbbeta3 receptors."
Thromb. Haemost. 92:1377-1386(2004) [PubMed] [Europe PMC] [Abstract]

"Double heterozygosity for a novel missense mutation of Ile304 to Asn in addition to the missense mutation His280 to Pro in the integrin beta3 gene as a cause of the absence of platelet alphaIIbbeta3 in Glanzmann's thrombasthenia."
J. Thromb. Haemost. 3:68-73(2005) [PubMed] [Europe PMC] [Abstract]

"Mutations in GPIIIa molecule as a cause for Glanzmann thrombasthenia in Indian patients."
J. Thromb. Haemost. 3:482-488(2005) [PubMed] [Europe PMC] [Abstract]

"A nonsynonymous SNP in the ITGB3 gene disrupts the conserved membrane-proximal cytoplasmic salt bridge in the alphaIIbbeta3 integrin and cosegregates dominantly with abnormal proplatelet formation and macrothrombocytopenia."
Blood 111:3407-3414(2008) [PubMed] [Europe PMC] [Abstract]

"AlphaIIbbeta3 integrin: new allelic variants in Glanzmann thrombasthenia, effects on ITGA2B and ITGB3 mRNA splicing, expression, and structure-function."
Hum. Mutat. 31:237-246(2010) [PubMed] [Europe PMC] [Abstract]