Focal Adhesion Kinase (FAK)
PTK2; FADK; FAK1; Pp125FAK; PPP1R71; Protein Tyrosine Kinase 2; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71
PTK2 is a focal adhesion-associated protein kinase involved in cellular adhesion and spreading processes. It has been shown that when FAK was blocked, breast cancer cells became less metastastic due to decreased mobility.FAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src. This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV499Hu01 | Recombinant Focal Adhesion Kinase (FAK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV499Hu01 | Monoclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IP. |
| PAV499Hu01 | Polyclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV499Hu01 | CLIA Kit for Focal Adhesion Kinase (FAK) | CLIA assay for Antigen Detection |
| ELV499Hu01 | ELISA Kit for Focal Adhesion Kinase (FAK) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV499Mu01 | Recombinant Focal Adhesion Kinase (FAK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV499Mu01 | Monoclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IP. |
| PAV499Mu01 | Polyclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV499Mu01 | CLIA Kit for Focal Adhesion Kinase (FAK) | CLIA assay for Antigen Detection |
| ELV499Mu01 | ELISA Kit for Focal Adhesion Kinase (FAK) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV499Ra01 | Recombinant Focal Adhesion Kinase (FAK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV499Ra01 | Monoclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IP. |
| PAV499Ra01 | Polyclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV499Ra01 | CLIA Kit for Focal Adhesion Kinase (FAK) | CLIA assay for Antigen Detection |
| ELV499Ra01 | ELISA Kit for Focal Adhesion Kinase (FAK) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV499Ca01 | Recombinant Focal Adhesion Kinase (FAK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV499Ca01 | Monoclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IP. |
| PAV499Ca01 | Polyclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV499Ca01 | CLIA Kit for Focal Adhesion Kinase (FAK) | CLIA assay for Antigen Detection |
| ELV499Ca01 | ELISA Kit for Focal Adhesion Kinase (FAK) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV499Po01 | Recombinant Focal Adhesion Kinase (FAK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV499Po01 | Monoclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IP. |
| PAV499Po01 | Polyclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV499Po01 | CLIA Kit for Focal Adhesion Kinase (FAK) | CLIA assay for Antigen Detection |
| ELV499Po01 | ELISA Kit for Focal Adhesion Kinase (FAK) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV499Bo01 | Recombinant Focal Adhesion Kinase (FAK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV499Bo01 | Monoclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IP. |
| PAV499Bo01 | Polyclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV499Bo01 | CLIA Kit for Focal Adhesion Kinase (FAK) | CLIA assay for Antigen Detection |
| ELV499Bo01 | ELISA Kit for Focal Adhesion Kinase (FAK) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV499Rb01 | Recombinant Focal Adhesion Kinase (FAK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV499Rb01 | Monoclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IP. |
| PAV499Rb01 | Polyclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV499Rb01 | CLIA Kit for Focal Adhesion Kinase (FAK) | CLIA assay for Antigen Detection |
| ELV499Rb01 | ELISA Kit for Focal Adhesion Kinase (FAK) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV499Mo01 | Recombinant Focal Adhesion Kinase (FAK) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV499Mo01 | Monoclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IP. |
| PAV499Mo01 | Polyclonal Antibody to Focal Adhesion Kinase (FAK) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV499Mo01 | CLIA Kit for Focal Adhesion Kinase (FAK) | CLIA assay for Antigen Detection |
| ELV499Mo01 | ELISA Kit for Focal Adhesion Kinase (FAK) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Human T and B lymphocytes express a structurally conserved focal adhesion kinase, pp125FAK."
DNA Cell Biol. 12:823-830(1993) [PubMed] [Europe PMC] [Abstract]
"Expression of an N-terminally truncated form of human focal adhesion kinase in brain."
Biochem. Biophys. Res. Commun. 190:140-147(1993) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"DNA sequence and analysis of human chromosome 8."
Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
, Submitted (JUL-2007) to UniProtKB
"A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes."
Oncogene 8:3403-3410(1993) [PubMed] [Europe PMC] [Abstract]
"Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions."
J. Biol. Chem. 273:1003-1014(1998) [PubMed] [Europe PMC] [Abstract]
"Interaction of Hic-5, A senescence-related protein, with focal adhesion kinase."
J. Biol. Chem. 273:26516-26521(1998) [PubMed] [Europe PMC] [Abstract]
"Activation of EphA2 kinase suppresses integrin function and causes focal-adhesion-kinase dephosphorylation."
Nat. Cell Biol. 2:62-69(2000) [PubMed] [Europe PMC] [Abstract]
"Regulation of the PH-domain-containing tyrosine kinase Etk by focal adhesion kinase through the FERM domain."
Nat. Cell Biol. 3:439-444(2001) [PubMed] [Europe PMC] [Abstract]
"Focal adhesion kinase enhances signaling through the Shc/extracellular signal-regulated kinase pathway in anaplastic astrocytoma tumor biopsy samples."
Cancer Res. 62:2699-2707(2002) [PubMed] [Europe PMC] [Abstract]
"Regulation of focal adhesion kinase by a novel protein inhibitor FIP200."
Mol. Biol. Cell 13:3178-3191(2002) [PubMed] [Europe PMC] [Abstract]
"Site-specific phosphorylation of platelet focal adhesion kinase by low-density lipoprotein."
Biochem. J. 369:407-416(2003) [PubMed] [Europe PMC] [Abstract]
"Focal adhesion kinase is upstream of phosphatidylinositol 3-kinase/Akt in regulating fibroblast survival in response to contraction of type I collagen matrices via a beta 1 integrin viability signaling pathway."
J. Biol. Chem. 279:33024-33034(2004) [PubMed] [Europe PMC] [Abstract]
"The proto-oncogene Fgr regulates cell migration and this requires its plasma membrane localization."
Exp. Cell Res. 302:253-269(2005) [PubMed] [Europe PMC] [Abstract]
"Direct interaction of the N-terminal domain of focal adhesion kinase with the N-terminal transactivation domain of p53."
J. Biol. Chem. 280:25008-25021(2005) [PubMed] [Europe PMC] [Abstract]
"Microtubule-induced focal adhesion disassembly is mediated by dynamin and focal adhesion kinase."
Nat. Cell Biol. 7:581-590(2005) [PubMed] [Europe PMC] [Abstract]
"Vascular endothelial growth factor receptor-3 and focal adhesion kinase bind and suppress apoptosis in breast cancer cells."
Cancer Res. 66:1446-1454(2006) [PubMed] [Europe PMC] [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
"Expression of FAK-related non-kinase (FRNK) coincides with morphological change in the early stage of cell adhesion."
Med. Mol. Morphol. 39:154-160(2006) [PubMed] [Europe PMC] [Abstract]
"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
"Therapeutic efficacy of a novel focal adhesion kinase inhibitor TAE226 in ovarian carcinoma."
Cancer Res. 67:10976-10983(2007) [PubMed] [Europe PMC] [Abstract]
"Cellular characterization of a novel focal adhesion kinase inhibitor."
J. Biol. Chem. 282:14845-14852(2007) [PubMed] [Europe PMC] [Abstract]
"Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin-5."
J. Cell. Biochem. 100:499-514(2007) [PubMed] [Europe PMC] [Abstract]
"Inhibition of both focal adhesion kinase and insulin-like growth factor-I receptor kinase suppresses glioma proliferation in vitro and in vivo."
Mol. Cancer Ther. 6:1357-1367(2007) [PubMed] [Europe PMC] [Abstract]
"The LIM protein leupaxin is enriched in smooth muscle and functions as an serum response factor cofactor to induce smooth muscle cell gene transcription."
Circ. Res. 102:1502-1511(2008) [PubMed] [Europe PMC] [Abstract]
"Etk/BMX, a Btk family tyrosine kinase, and Mal contribute to the cross-talk between MyD88 and FAK pathways."
J. Immunol. 180:3485-3491(2008) [PubMed] [Europe PMC] [Abstract]
"A novel Cas family member, HEPL, regulates FAK and cell spreading."
Mol. Biol. Cell 19:1627-1636(2008) [PubMed] [Europe PMC] [Abstract]
"Nuclear FAK promotes cell proliferation and survival through FERM-enhanced p53 degradation."
Mol. Cell 29:9-22(2008) [PubMed] [Europe PMC] [Abstract]
"Regulation of estrogen rapid signaling through arginine methylation by PRMT1."
Mol. Cell 31:212-221(2008) [PubMed] [Europe PMC] [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
"Specific tyrosine phosphorylation of focal adhesion kinase mediated by Fer tyrosine kinase in suspended hepatocytes."
Biochim. Biophys. Acta 1793:781-791(2009) [PubMed] [Europe PMC] [Abstract]
"Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion."
BMC Cancer 9:12-12(2009) [PubMed] [Europe PMC] [Abstract]
"STEAP4 regulates focal adhesion kinase activation and CpG motifs within STEAP4 promoter region are frequently methylated in DU145, human androgen-independent prostate cancer cells."
Int. J. Mol. Med. 24:599-604(2009) [PubMed] [Europe PMC] [Abstract]
"Functional consequences of interactions between FAK and epithelial membrane protein 2 (EMP2)."
Invest. Ophthalmol. Vis. Sci. 50:4949-4956(2009) [PubMed] [Europe PMC] [Abstract]
"Ras- and PI3K-dependent breast tumorigenesis in mice and humans requires focal adhesion kinase signaling."
J. Clin. Invest. 119:252-266(2009) [PubMed] [Europe PMC] [Abstract]
"EphA1 interacts with integrin-linked kinase and regulates cell morphology and motility."
J. Cell Sci. 122:243-255(2009) [PubMed] [Europe PMC] [Abstract]
"Large-scale proteomics analysis of the human kinome."
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
"Bioluminescent imaging study: FAK inhibitor, PF-562,271, preclinical study in PC3M-luc-C6 local implant and metastasis xenograft models."
Cancer Biol. Ther. 10:38-43(2010) [PubMed] [Europe PMC] [Abstract]
"LIM domain-containing adaptor, leupaxin, localizes in focal adhesion and suppresses the integrin-induced tyrosine phosphorylation of paxillin."
Cancer Sci. 101:363-368(2010) [PubMed] [Europe PMC] [Abstract]
"Downregulation of FAK-related non-kinase mediates the migratory phenotype of human fibrotic lung fibroblasts."
Exp. Cell Res. 316:1600-1609(2010) [PubMed] [Europe PMC] [Abstract]
"ZF21 protein regulates cell adhesion and motility."
J. Biol. Chem. 285:21013-21022(2010) [PubMed] [Europe PMC] [Abstract]
"CD36 ligands promote sterile inflammation through assembly of a Toll-like receptor 4 and 6 heterodimer."
Nat. Immunol. 11:155-161(2010) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
"Focal adhesion kinase (FAK) binds RET kinase via its FERM domain, priming a direct and reciprocal RET-FAK transactivation mechanism."
J. Biol. Chem. 286:17292-17302(2011) [PubMed] [Europe PMC] [Abstract]
"A novel splice variant of calcium and integrin-binding protein 1 mediates protein kinase D2-stimulated tumour growth by regulating angiogenesis."
Oncogene 33:1167-1180(2014) [PubMed] [Europe PMC] [Abstract]
"Signaling through focal adhesion kinase."
Prog. Biophys. Mol. Biol. 71:435-478(1999) [PubMed] [Europe PMC] [Abstract]
"Netrin-integrin signaling in epithelial morphogenesis, axon guidance and vascular patterning."
Cell Cycle 4:E131-E135(2005) [PubMed] [Europe PMC] [Abstract]
"Integrin-regulated FAK-Src signaling in normal and cancer cells."
Curr. Opin. Cell Biol. 18:516-523(2006) [PubMed] [Europe PMC] [Abstract]
"Focal adhesion kinase: an essential kinase in the regulation of cardiovascular functions."
IUBMB Life 59:709-716(2007) [PubMed] [Europe PMC] [Abstract]
"FERM control of FAK function: implications for cancer therapy."
Cell Cycle 7:2306-2314(2008) [PubMed] [Europe PMC] [Abstract]
"Focal adhesion kinase: switching between GAPs and GEFs in the regulation of cell motility."
Curr. Opin. Cell Biol. 21:676-683(2009) [PubMed] [Europe PMC] [Abstract]
"Focal adhesion kinase and cancer."
Histol. Histopathol. 24:503-510(2009) [PubMed] [Europe PMC] [Abstract]
"Focal adhesion kinase and p53 signal transduction pathways in cancer."
Front. Biosci. 15:901-912(2010) [PubMed] [Europe PMC] [Abstract]
"The role of focal adhesion kinase in early development."
Histol. Histopathol. 25:1039-1055(2010) [PubMed] [Europe PMC] [Abstract]
"Integrin signaling through FAK in the regulation of mammary stem cells and breast cancer."
IUBMB Life 62:268-276(2010) [PubMed] [Europe PMC] [Abstract]
"Cellular functions of FAK kinases: insight into molecular mechanisms and novel functions."
J. Cell Sci. 123:1007-1013(2010) [PubMed] [Europe PMC] [Abstract]
"Focal adhesion kinase: exploring Fak structure to gain insight into function."
Int. Rev. Cell Mol. Biol. 288:185-225(2011) [PubMed] [Europe PMC] [Abstract]
"MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression."
J. Cell Biol. 200:773-787(2013) [PubMed] [Europe PMC] [Abstract]
"The structural basis of localization and signaling by the focal adhesion targeting domain."
Structure 10:319-327(2002) [PubMed] [Europe PMC] [Abstract]
"Structures of the cancer-related Aurora-A, FAK, and EphA2 protein kinases from nanovolume crystallography."
Structure 10:1659-1667(2002) [PubMed] [Europe PMC] [Abstract]
"Molecular recognition of paxillin LD motifs by the focal adhesion targeting domain."
Structure 11:1207-1217(2003) [PubMed] [Europe PMC] [Abstract]
"Antitumor activity and pharmacology of a selective focal adhesion kinase inhibitor, PF-562,271."
Cancer Res. 68:1935-1944(2008) [PubMed] [Europe PMC] [Abstract]
"Structural basis for the interaction between focal adhesion kinase and CD4."
J. Mol. Biol. 375:1320-1328(2008) [PubMed] [Europe PMC] [Abstract]
"Patterns of somatic mutation in human cancer genomes."
Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]