Eukaryotic Translation Initiation Factor 3A (EIF3A)

EIF3S10; EIF3-A; EIF3; P167; EIF3-P170; EIF3-Theta; P180; P185; Eukaryotic translation initiation factor 3 subunit 10

SPN	U956
HGNC	3271
MGI	95301
PubMed	20647036
UniProt	Q14152
Wiki	EIF3A

EIF3, at 650 kD, is the largest of the EIFs. According to Johnson et al. (1997), EIF3 has been implicated in several roles, including binding to the 40S ribosomal subunit and to other EIFs, possibly to align the factors for initial binding to the 40S subunit and the subsequent identification of the AUG initiation codon. The EIF3 protein synthesis initiation factor is composed of at least 8 subunits, the largest of which is p180.Using a library of endoribonuclease-prepared short interfering RNAs (esiRNAs), Kittler et al. (2004) identified 37 genes required for cell division, one of which was EIF3S10. These 37 genes included several splicing factors for which knockdown generates mitotic spindle defects. In addition, a putative nuclear-export terminator was found to speed up cell proliferation and mitotic progression after knockdown.

Reference

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Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]

, Submitted (JUL-2007) to UniProtKB

"Molecular interaction between human tumor marker protein p150, the largest subunit of eIF3, and intermediate filament protein K7."
J. Cell. Biochem. 80:483-490(2001) [PubMed] [Europe PMC] [Abstract]

"Characterization of eIF3k: a newly discovered subunit of mammalian translation initiation factor eIF3."
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"The j-subunit of human translation initiation factor eIF3 is required for the stable binding of eIF3 and its subcomplexes to 40 S ribosomal subunits in vitro."
J. Biol. Chem. 279:8946-8956(2004) [PubMed] [Europe PMC] [Abstract]

"Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association."
RNA 11:470-486(2005) [PubMed] [Europe PMC] [Abstract]

"Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit."
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EMBO J. 26:3373-3383(2007) [PubMed] [Europe PMC] [Abstract]

"Human INT6/eIF3e is required for nonsense-mediated mRNA decay."
EMBO Rep. 8:596-602(2007) [PubMed] [Europe PMC] [Abstract]

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Mol. Cell. Proteomics 6:1135-1146(2007) [PubMed] [Europe PMC] [Abstract]

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Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3."
Proc. Natl. Acad. Sci. U.S.A. 105:18139-18144(2008) [PubMed] [Europe PMC] [Abstract]

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"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
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"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Structural roles for human translation factor eIF3 in initiation of protein synthesis."
Science 310:1513-1515(2005) [PubMed] [Europe PMC] [Abstract]