Barrier To Autointegration Factor 1 (BANF1)

BAF; BCRP1; Breakpoint cluster region protein 1; Barrier-to-autointegration factor, N-terminally processed

SPN	U510
HGNC	17397
MGI	1346330
PubMed	21750191
RGD	620662
UniProt	O75531
Wiki	BANF1

Barrier-to-autointegration factor was identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The endogenous function of the protein is unknown.

The protein forms a homodimer which localizes to the nucleus and is specifically associated with chromosomes during mitosis. This protein binds to DNA in a non-specific manner and studies in rodents suggest that it also binds to lamina-associated polypeptide 2, a component of the nuclear lamina. It also associates with the LEM Domain containing proteins LAP2, Emerin, and MAN1.

Reference

"A previously unidentified host protein protects retroviral DNA from autointegration."
Proc. Natl. Acad. Sci. U.S.A. 95:1528-1533(1998) [PubMed] [Europe PMC] [Abstract]

"Genomic and functional map of the chromosome 14 t(12;14) breakpoint cluster region in uterine leiomyoma."
Genomics 52:17-26(1998) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Both the structure and DNA binding function of the barrier-to-autointegration factor contribute to reconstitution of HIV type 1 integration in vitro."
J. Biol. Chem. 275:39671-39677(2000) [PubMed] [Europe PMC] [Abstract]

"Barrier-to-autointegration factor (BAF) bridges DNA in a discrete, higher-order nucleoprotein complex."
Proc. Natl. Acad. Sci. U.S.A. 97:8997-9002(2000) [PubMed] [Europe PMC] [Abstract]

"BAF is required for emerin assembly into the reforming nuclear envelope."
J. Cell Sci. 114:4575-4585(2001) [PubMed] [Europe PMC] [Abstract]

"Barrier-to-autointegration factor: major roles in chromatin decondensation and nuclear assembly."
J. Cell Biol. 158:475-485(2002) [PubMed] [Europe PMC] [Abstract]

"The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes."
J. Virol. 77:5030-5036(2003) [PubMed] [Europe PMC] [Abstract]

"Barrier-to-autointegration factor BAF binds p55 Gag and matrix and is a host component of human immunodeficiency virus type 1 virions."
J. Virol. 77:13084-13092(2003) [PubMed] [Europe PMC] [Abstract]

"Direct binding of nuclear membrane protein MAN1 to emerin in vitro and two modes of binding to barrier-to-autointegration factor."
J. Biol. Chem. 280:13863-13870(2005) [PubMed] [Europe PMC] [Abstract]

"Binding of barrier to autointegration factor (BAF) to histone H3 and selected linker histones including H1.1."
J. Biol. Chem. 280:42252-42262(2005) [PubMed] [Europe PMC] [Abstract]

"Barrier-to-autointegration factor phosphorylation on Ser-4 regulates emerin binding to lamin A in vitro and emerin localization in vivo."
Mol. Biol. Cell 17:1154-1163(2006) [PubMed] [Europe PMC] [Abstract]

"Barrier-to-autointegration factor-like (BAF-L): a proposed regulator of BAF."
Exp. Cell Res. 312:478-487(2006) [PubMed] [Europe PMC] [Abstract]

"The vaccinia-related kinases phosphorylate the N' terminus of BAF, regulating its interaction with DNA and its retention in the nucleus."
Mol. Biol. Cell 17:2451-2464(2006) [PubMed] [Europe PMC] [Abstract]

"The inner-nuclear-envelope protein emerin regulates HIV-1 infectivity."
Nature 441:641-645(2006) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Coordination of kinase and phosphatase activities by Lem4 enables nuclear envelope reassembly during mitosis."
Cell 150:122-135(2012) [PubMed] [Europe PMC] [Abstract]

"BAF: roles in chromatin, nuclear structure and retrovirus integration."
Trends Cell Biol. 14:261-266(2004) [PubMed] [Europe PMC] [Abstract]

"Solution structure of the cellular factor BAF responsible for protecting retroviral DNA from autointegration."
Nat. Struct. Biol. 5:903-909(1998) [PubMed] [Europe PMC] [Abstract]

"Structural basis of DNA bridging by barrier-to-autointegration factor."
Biochemistry 39:9130-9138(2000) [PubMed] [Europe PMC] [Abstract]

"Exome sequencing and functional analysis identifies BANF1 mutation as the cause of a hereditary progeroid syndrome."
Am. J. Hum. Genet. 88:650-656(2011) [PubMed] [Europe PMC] [Abstract]