Trinucleotide Repeat Containing Protein 6A (TNRC6A)

CAGH26; GW1; GW182; TNRC6; CAG repeat protein 26; EMSY interactor protein; GW182 autoantigen; Glycine-tryptophan protein of 182 kDa

SPN	U056
HGNC	11969
MGI	2385292
PubMed	23150874
RGD	1307268
UniProt	Q8NDV7
Wiki	TNRC6A

Trinucleotide repeat-containing gene 6A protein is a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.

Reference

"A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles."
Mol. Biol. Cell 13:1338-1351(2002) [PubMed] [Europe PMC] [Abstract]

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BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]

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Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"cDNAs with long CAG trinucleotide repeats from human brain."
Hum. Genet. 100:114-122(1997) [PubMed] [Europe PMC] [Abstract]

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DNA Res. 7:143-150(2000) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"Clinical and serological associations of autoantibodies to GW bodies and a novel cytoplasmic autoantigen GW182."
J. Mol. Med. 81:811-818(2003) [PubMed] [Europe PMC] [Abstract]

"The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies."
RNA 9:1171-1173(2003) [PubMed] [Europe PMC] [Abstract]

"GW182 is critical for the stability of GW bodies expressed during the cell cycle and cell proliferation."
J. Cell Sci. 117:5567-5578(2004) [PubMed] [Europe PMC] [Abstract]

"Argonaute 2/RISC resides in sites of mammalian mRNA decay known as cytoplasmic bodies."
Nat. Cell Biol. 7:633-636(2005) [PubMed] [Europe PMC] [Abstract]

"A role for the P-body component GW182 in microRNA function."
Nat. Cell Biol. 7:1261-1266(2005) [PubMed] [Europe PMC] [Abstract]

"Disruption of GW bodies impairs mammalian RNA interference."
Nat. Cell Biol. 7:1267-1274(2005) [PubMed] [Europe PMC] [Abstract]

Erratum
Nat. Cell Biol. 8:100-100(2006)

"Formation of GW bodies is a consequence of microRNA genesis."
EMBO Rep. 7:904-910(2006) [PubMed] [Europe PMC] [Abstract]

"Let-7 microRNA-mediated mRNA deadenylation and translational repression in a mammalian cell-free system."
Genes Dev. 21:1857-1862(2007) [PubMed] [Europe PMC] [Abstract]

Erratum
Genes Dev. 21:2509-2509(2007)

"Small interfering RNA-mediated silencing induces target-dependent assembly of GW/P bodies."
Mol. Biol. Cell 18:3375-3387(2007) [PubMed] [Europe PMC] [Abstract]

Erratum
Mol. Biol. Cell 18:4200-4200(2007)

"Identification of GW182 and its novel isoform TNGW1 as translational repressors in Ago2-mediated silencing."
J. Cell Sci. 121:4134-4144(2008) [PubMed] [Europe PMC] [Abstract]

"Prolyl 4-hydroxylation regulates Argonaute 2 stability."
Nature 455:421-424(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Localization of double-stranded small interfering RNA to cytoplasmic processing bodies is Ago2 dependent and results in up-regulation of GW182 and Argonaute-2."
Mol. Biol. Cell 20:521-529(2009) [PubMed] [Europe PMC] [Abstract]

"Importance of the C-terminal domain of the human GW182 protein TNRC6C for translational repression."
RNA 15:781-793(2009) [PubMed] [Europe PMC] [Abstract]

"The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing."
RNA 15:804-813(2009) [PubMed] [Europe PMC] [Abstract]

"The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts independently of Argonaute proteins."
RNA 15:1059-1066(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"GW182 proteins directly recruit cytoplasmic deadenylase complexes to miRNA targets."
Mol. Cell 44:120-133(2011) [PubMed] [Europe PMC] [Abstract]