Myosin Light Chain 12B (MYL12B)

MRLC2; MLC20; SHUJUN-1; Myosin Regulatory Light Chain 2; Myosin regulatory light chain 2-B, smooth muscle isoform; Myosin regulatory light chain 20 kDa

SPN	T345
HGNC	29827
MGI	107494
PubMed	17091196
RGD	628855
UniProt	O14950
Wiki	Myl12b

The activity of nonmuscle myosin II is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments.Immunolocalization of the mutant proteins in dividing cells revealed colocalization of the 2 phosphorylated forms with actin filaments in the peripheral region and with the contractile ring, while the unphosphorylated form localized diffusely throughout the cytoplasm. Iwasaki et al. (2001) concluded that phosphorylation of MRLC2 is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells. They suggested that diphosphorylated MRLC may be more effective in organizing actin filaments than monophosphorylated MRLC.

Reference

"Diphosphorylated MRLC is required for organization of stress fibers in interphase cells and the contractile ring in dividing cells."
Cell Struct. Funct. 26:677-683(2001) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning and sequencing of myosin light chains in human megakaryoblastic leukemia cells."
J. Smooth Muscle Res. 37:25-38(2001) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Activation of actin-activated MgATPase activity of myosin II by phosphorylation with MAPK-activated protein kinase-1b (RSK-2)."
J. Biochem. 128:435-440(2000) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation of myosin II regulatory light chain is necessary for migration of HeLa cells but not for localization of myosin II at the leading edge."
Biochem. J. 370:551-556(2003) [PubMed] [Europe PMC] [Abstract]

"Myosin motors and not actin comets are mediators of the actin-based Golgi-to-endoplasmic reticulum protein transport."
Mol. Biol. Cell 14:445-459(2003) [PubMed] [Europe PMC] [Abstract]

"Epigallocatechin-3-O-gallate disrupts stress fibers and the contractile ring by reducing myosin regulatory light chain phosphorylation mediated through the target molecule 67 kDa laminin receptor."
Biochem. Biophys. Res. Commun. 333:628-635(2005) [PubMed] [Europe PMC] [Abstract]

"Novel ZIP kinase isoform lacks leucine zipper."
Arch. Biochem. Biophys. 456:194-203(2006) [PubMed] [Europe PMC] [Abstract]

"Smooth muscle type isoform of 20 kDa myosin light chain is expressed in monocyte/macrophage cell lineage."
J. Smooth Muscle Res. 44:29-40(2008) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]