RNA Binding Motif Protein 8 (RBM8)

RBM8A; BOV-1A; BOV-1B; BOV-1C; RBM8B; Y14; ZNRP; ZRNP1; Binder of OVCA1-1; RNA-binding protein Y14; Ribonucleoprotein RBM8A

SPN	Q151
HGNC	9905
MGI	1913129
PubMed	10662555
RGD	1310099
UniProt	Q9Y5S9
Wiki	RBM8A

RBM8 encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events.

Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.

Reference

"Cloning and gene expression of a novel human ribonucleoprotein."
Biochim. Biophys. Acta 1492:465-469(2000) [PubMed] [Europe PMC] [Abstract]

"MAGOH interacts with a novel RNA-binding protein."
Genomics 63:145-148(2000) [PubMed] [Europe PMC] [Abstract]

"Identification and structural analysis of human RBM8A and RBM8B: two highly conserved RNA-binding motif proteins that interact with OVCA1, a candidate tumor suppressor."
Genomics 69:54-62(2000) [PubMed] [Europe PMC] [Abstract]

"Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm."
Mol. Cell 6:673-682(2000) [PubMed] [Europe PMC] [Abstract]

"The genes encoding the type II gonadotropin-releasing hormone receptor and the ribonucleoprotein RBM8A in humans overlap in two genomic loci."
Genomics 78:15-18(2001) [PubMed] [Europe PMC] [Abstract]

"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells."
Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]

"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions."
EMBO J. 19:6860-6869(2000) [PubMed] [Europe PMC] [Abstract]

"Importin 13: a novel mediator of nuclear import and export."
EMBO J. 20:3685-3694(2001) [PubMed] [Europe PMC] [Abstract]

"Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon-exon junction complex."
EMBO J. 20:6424-6433(2001) [PubMed] [Europe PMC] [Abstract]

"Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex."
Science 293:1832-1836(2001) [PubMed] [Europe PMC] [Abstract]

"Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1."
Science 293:1836-1839(2001) [PubMed] [Europe PMC] [Abstract]

"Translation is required to remove Y14 from mRNAs in the cytoplasm."
Curr. Biol. 12:1060-1067(2002) [PubMed] [Europe PMC] [Abstract]

"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis."
RNA 8:426-439(2002) [PubMed] [Europe PMC] [Abstract]

"An evolutionarily conserved role for SRm160 in 3'-end processing that functions independently of exon junction complex formation."
J. Biol. Chem. 278:44153-44160(2003) [PubMed] [Europe PMC] [Abstract]

"Y14 and hUpf3b form an NMD-activating complex."
Mol. Cell 11:939-949(2003) [PubMed] [Europe PMC] [Abstract]

"A novel mode of RBD-protein recognition in the Y14-Mago complex."
Nat. Struct. Biol. 10:433-439(2003) [PubMed] [Europe PMC] [Abstract]

"Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex."
EMBO Rep. 5:304-310(2004) [PubMed] [Europe PMC] [Abstract]

"A simple whole cell lysate system for in vitro splicing reveals a stepwise assembly of the exon-exon junction complex."
J. Biol. Chem. 279:7009-7013(2004) [PubMed] [Europe PMC] [Abstract]

"Exon-junction complex components specify distinct routes of nonsense-mediated mRNA decay with differential cofactor requirements."
Mol. Cell 20:65-75(2005) [PubMed] [Europe PMC] [Abstract]

"The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity."
Nat. Struct. Mol. Biol. 12:861-869(2005) [PubMed] [Europe PMC] [Abstract]

"Biochemical analysis of the EJC reveals two new factors and a stable tetrameric protein core."
RNA 11:1869-1883(2005) [PubMed] [Europe PMC] [Abstract]

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

"PYM binds the cytoplasmic exon-junction complex and ribosomes to enhance translation of spliced mRNAs."
Nat. Struct. Mol. Biol. 14:1173-1179(2007) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Exon junction complex enhances translation of spliced mRNAs at multiple steps."
Biochem. Biophys. Res. Commun. 384:334-340(2009) [PubMed] [Europe PMC] [Abstract]

"Disassembly of exon junction complexes by PYM."
Cell 137:536-548(2009) [PubMed] [Europe PMC] [Abstract]

"Assembly and mobility of exon-exon junction complexes in living cells."
RNA 15:862-876(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"Proteins associated with the exon junction complex also control the alternative splicing of apoptotic regulators."
Mol. Cell. Biol. 32:954-967(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Structure of the Y14-Magoh core of the exon junction complex."
Curr. Biol. 13:933-941(2003) [PubMed] [Europe PMC] [Abstract]

"The crystal structure of the exon junction complex reveals how it maintains a stable grip on mRNA."
Cell 126:713-725(2006) [PubMed] [Europe PMC] [Abstract]

"Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA."
Science 313:1968-1972(2006) [PubMed] [Europe PMC] [Abstract]

"Mechanism of ATP turnover inhibition in the EJC."
RNA 15:67-75(2009) [PubMed] [Europe PMC] [Abstract]