Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5)

CGI-70; UCH37; INO80R; INO80 Complex Subunit R; Ubiquitin thioesterase L5; Ubiquitin C-terminal hydrolase UCH37

SPN	P803
HGNC	19678
MGI	1914848
PubMed	24319254
RGD	1305414
UniProt	Q9Y5K5
Wiki	UCHL5

UCHL5 is a member of the UCH enzyme family and contains a conserved catalytic domain and a nonconserved extended C-terminal tail.The activity of UCHL5 was significantly increased in 76% of the tumors analyzed. UCHL5 sequentially removes ubiquitin from the distal end of the lys48-linked polyubiquitin chain and has the potential to rescue ubiquitinated substrates from proteasomal degradation. UCHL5 was active in 92% (24 of 26) of the cervical carcinoma biopsies, and in 100% of 8 cervical carcinoma cell lines, of which 6 were HPV-positive and 2 were HPV-negative. UCHL5 activity level did not correlate with the clinical stage of the tumors or the presence of metastases.Uchl5 overexpression increases Tgfbr1-dependent transcription. Knockdown of Uchl5 expression by RNA interference abrogated Tgf-beta-dependent transcription.

Reference

"Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1."
Nat. Cell Biol. 8:994-1002(2006) [PubMed] [Europe PMC] [Abstract]

"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics."
Genome Res. 10:703-713(2000) [PubMed] [Europe PMC] [Abstract]

"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning."
Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"A novel proteasome-interacting protein recruits the deubiquitinating enzyme UCH37 to 26S proteasomes."
EMBO J. 25:4524-4536(2006) [PubMed] [Europe PMC] [Abstract]

"hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds the deubiquitinating enzyme, UCH37."
EMBO J. 25:5742-5753(2006) [PubMed] [Europe PMC] [Abstract]

"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex."
Biochemistry 46:3553-3565(2007) [PubMed] [Europe PMC] [Abstract]

"Distinct modes of regulation of the Uch37 deubiquitinating enzyme in the proteasome and in the Ino80 chromatin-remodeling complex."
Mol. Cell 31:909-917(2008) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Subunit organization of the human INO80 chromatin remodeling complex: An evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling."
J. Biol. Chem. 286:11283-11289(2011) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of the de-ubiquitinating enzyme UCH37 (human UCH-L5) catalytic domain."
Biochem. Biophys. Res. Commun. 390:855-860(2009) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of UCH37."
Submitted (AUG-2009) to the PDB data bank