Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1)
SIT; Suppression Inducing Transmembrane adaptor 1; SHP2 Interacting Transmembrane Adaptor
Sequence analysis predicted that SIT has a putative 22-amino acid hydrophobic leader peptide, an 18-amino acid extracellular domain containing a potential N-glycosylation site and a cys residue that may be involved in an interchain disulfide bond, a 20-amino acid transmembrane domain, and a 136-amino acid intracellular portion containing several potential phosphorylation sites.
SIT also contains 6 tyrosine residues, 5 of which may be involved in Src homology-2 (SH2) domain-mediated protein-protein interactions after phosphorylation. The cytoplasmic portion of SIT possesses a potential immunoreceptor tyrosine-based inhibitory motif, or ITIM, suggesting the potential for interactions with SHP1 (PTPN6) or SHP2 (PTPN11).
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP576Hu01 | Recombinant Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP576Hu01 | Monoclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IP. |
| PAP576Hu01 | Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP576Hu01 | CLIA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | CLIA assay for Antigen Detection |
| ELP576Hu01 | ELISA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP576Mu01 | Recombinant Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP576Mu01 | Monoclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IP. |
| PAP576Mu01 | Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP576Mu01 | CLIA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | CLIA assay for Antigen Detection |
| ELP576Mu01 | ELISA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP576Ra01 | Recombinant Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP576Ra01 | Monoclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IP. |
| PAP576Ra01 | Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP576Ra01 | CLIA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | CLIA assay for Antigen Detection |
| ELP576Ra01 | ELISA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP576Ca01 | Recombinant Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP576Ca01 | Monoclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IP. |
| PAP576Ca01 | Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP576Ca01 | CLIA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | CLIA assay for Antigen Detection |
| ELP576Ca01 | ELISA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP576Po01 | Recombinant Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP576Po01 | Monoclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IP. |
| PAP576Po01 | Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP576Po01 | CLIA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | CLIA assay for Antigen Detection |
| ELP576Po01 | ELISA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP576Bo01 | Recombinant Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP576Bo01 | Monoclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IP. |
| PAP576Bo01 | Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP576Bo01 | CLIA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | CLIA assay for Antigen Detection |
| ELP576Bo01 | ELISA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP576Rb01 | Recombinant Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP576Rb01 | Monoclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IP. |
| PAP576Rb01 | Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP576Rb01 | CLIA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | CLIA assay for Antigen Detection |
| ELP576Rb01 | ELISA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPP576Mo01 | Recombinant Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAP576Mo01 | Monoclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IP. |
| PAP576Mo01 | Polyclonal Antibody to Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLP576Mo01 | CLIA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | CLIA assay for Antigen Detection |
| ELP576Mo01 | ELISA Kit for Signaling Threshold Regulating Transmembrane Adaptor 1 (SIT1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T-cell activation."
J. Exp. Med. 189:1181-1194(1999) [PubMed] [Europe PMC] [Abstract]
"Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2-interacting transmembrane adaptor protein (SIT)."
Immunogenetics 53:337-341(2001) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"DNA sequence and analysis of human chromosome 9."
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Structural and functional dissection of the cytoplasmic domain of the transmembrane adaptor protein SIT (SHP2-interacting transmembrane adaptor protein)."
Eur. J. Immunol. 31:1825-1836(2001) [PubMed] [Europe PMC] [Abstract]
"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
"Transmembrane adaptor molecules: a new category of lymphoid-cell markers."
Blood 107:213-221(2006) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]