Ubiquitin Cross Reactive Protein (UCRP)

G1P2; IFI15; ISG15; Interferon Alpha-Inducible Protein clone IFI-15K; Interferon Induced Protein 15; Interferon-induced 17 kDa protein

SPN	M757
HGNC	4053
MGI	1855694
PubMed	15670748
RGD	1310312
UniProt	P05161
Wiki	ISG15

ISG15 shares several common properties with other ubiquitin-like molecules (UBLs), but its activity is tightly regulated by specific signaling pathways that have a role in innate immunity. ISG15 was identified as an interferon stimulated gene (ISG) since its expression is induced in response to type I interferons or lipopolysaccharide treatment. Upon interferon treatment, ISG15 can be detected in both free and conjugated forms, and is secreted from monocytes and lymphocytes where it can function as a cytokine. In the cell, ISG15 co-localizes with intermediate filaments and ISGylation may modulate the JAK-STAT pathway or certain aspects of neurological disease. It is also known as UCRP (ubiquitin cross-reactive protein) since it contains 2 tandem ubiquitin homology domains and is cross-reactive with ubiquitin antibodies.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM757Hu01	Recombinant Ubiquitin Cross Reactive Protein (UCRP)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM757Hu01	Monoclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IP.
Antibodies	PAM757Hu01	Polyclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IF.
Assay Kits	CLM757Hu01	CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)	CLIA assay for Antigen Detection
Assay Kits	ELM757Hu01	ELISA Kit for Ubiquitin Cross Reactive Protein (UCRP)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM757Mu01	Recombinant Ubiquitin Cross Reactive Protein (UCRP)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM757Mu01	Monoclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IP.
Antibodies	PAM757Mu01	Polyclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IF.
Assay Kits	CLM757Mu01	CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)	CLIA assay for Antigen Detection
Assay Kits	ELM757Mu01	ELISA Kit for Ubiquitin Cross Reactive Protein (UCRP)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM757Ra01	Recombinant Ubiquitin Cross Reactive Protein (UCRP)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM757Ra01	Monoclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IP.
Antibodies	PAM757Ra01	Polyclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IF.
Assay Kits	CLM757Ra01	CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)	CLIA assay for Antigen Detection
Assay Kits	ELM757Ra01	ELISA Kit for Ubiquitin Cross Reactive Protein (UCRP)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM757Ca01	Recombinant Ubiquitin Cross Reactive Protein (UCRP)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM757Ca01	Monoclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IP.
Antibodies	PAM757Ca01	Polyclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IF.
Assay Kits	CLM757Ca01	CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)	CLIA assay for Antigen Detection
Assay Kits	ELM757Ca01	ELISA Kit for Ubiquitin Cross Reactive Protein (UCRP)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM757Po01	Recombinant Ubiquitin Cross Reactive Protein (UCRP)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM757Po01	Monoclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IP.
Antibodies	PAM757Po01	Polyclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IF.
Assay Kits	CLM757Po01	CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)	CLIA assay for Antigen Detection
Assay Kits	ELM757Po01	ELISA Kit for Ubiquitin Cross Reactive Protein (UCRP)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM757Bo01	Recombinant Ubiquitin Cross Reactive Protein (UCRP)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM757Bo01	Monoclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IP.
Antibodies	PAM757Bo01	Polyclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IF.
Assay Kits	CLM757Bo01	CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)	CLIA assay for Antigen Detection
Assay Kits	ELM757Bo01	ELISA Kit for Ubiquitin Cross Reactive Protein (UCRP)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM757Rb01	Recombinant Ubiquitin Cross Reactive Protein (UCRP)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM757Rb01	Monoclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IP.
Antibodies	PAM757Rb01	Polyclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IF.
Assay Kits	CLM757Rb01	CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)	CLIA assay for Antigen Detection
Assay Kits	ELM757Rb01	ELISA Kit for Ubiquitin Cross Reactive Protein (UCRP)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM757Mo01	Recombinant Ubiquitin Cross Reactive Protein (UCRP)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM757Mo01	Monoclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IP.
Antibodies	PAM757Mo01	Polyclonal Antibody to Ubiquitin Cross Reactive Protein (UCRP)	WB; IHC; ICC; IF.
Assay Kits	CLM757Mo01	CLIA Kit for Ubiquitin Cross Reactive Protein (UCRP)	CLIA assay for Antigen Detection
Assay Kits	ELM757Mo01	ELISA Kit for Ubiquitin Cross Reactive Protein (UCRP)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Molecular characterization of the interferon-induced 15-kDa protein. Molecular cloning and nucleotide and amino acid sequence."
J. Biol. Chem. 261:8811-8816(1986) [PubMed] [Europe PMC] [Abstract]

"Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element."
Proc. Natl. Acad. Sci. U.S.A. 84:6394-6398(1987) [PubMed] [Europe PMC] [Abstract]

"A 15-kDa interferon-induced protein is derived by COOH-terminal processing of a 17-kDa precursor."
J. Biol. Chem. 263:4520-4522(1988) [PubMed] [Europe PMC] [Abstract]

Erratum
J. Biol. Chem. 263:10040-10040(1988)

"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"A 15-kD interferon-induced protein and its 17-kD precursor: expression in Escherichia coli, purification, and characterization."
J. Interferon Res. 9:493-507(1989) [PubMed] [Europe PMC] [Abstract]

"Interferon induces a 15-kilodalton protein exhibiting marked homology to ubiquitin."
J. Biol. Chem. 262:11315-11323(1987) [PubMed] [Europe PMC] [Abstract]

"The interferon-inducible 15-kDa ubiquitin homolog conjugates to intracellular proteins."
J. Biol. Chem. 267:7806-7813(1992) [PubMed] [Europe PMC] [Abstract]

"Conjugates of ubiquitin cross-reactive protein distribute in a cytoskeletal pattern."
Mol. Cell. Biol. 14:8408-8419(1994) [PubMed] [Europe PMC] [Abstract]

"Conjugation of the 15-kDa interferon-induced ubiquitin homolog is distinct from that of ubiquitin."
J. Biol. Chem. 271:324-330(1996) [PubMed] [Europe PMC] [Abstract]

"IFN-induced 15-kDa protein is released from human lymphocytes and monocytes."
J. Immunol. 146:2280-2284(1991) [PubMed] [Europe PMC] [Abstract]

"Immunohistochemical localization of ubiquitin cross-reactive protein in human tissues."
J. Pathol. 177:163-169(1995) [PubMed] [Europe PMC] [Abstract]

"Influenza B virus NS1 protein inhibits conjugation of the interferon (IFN)-induced ubiquitin-like ISG15 protein."
EMBO J. 20:362-371(2001) [PubMed] [Europe PMC] [Abstract]

"UBP43 (USP18) specifically removes ISG15 from conjugated proteins."
J. Biol. Chem. 277:9976-9981(2002) [PubMed] [Europe PMC] [Abstract]

"The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-alpha/beta-induced ubiquitin-like protein."
Proc. Natl. Acad. Sci. U.S.A. 101:7578-7582(2004) [PubMed] [Europe PMC] [Abstract]

"ISG15 modification of Ubc13 suppresses its ubiquitin-conjugating activity."
Biochem. Biophys. Res. Commun. 336:9-13(2005) [PubMed] [Europe PMC] [Abstract]

"Link between the ubiquitin conjugation system and the ISG15 conjugation system: ISG15 conjugation to the UbcH6 ubiquitin E2 enzyme."
J. Biochem. 138:711-719(2005) [PubMed] [Europe PMC] [Abstract]

"Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways."
Proc. Natl. Acad. Sci. U.S.A. 102:10200-10205(2005) [PubMed] [Europe PMC] [Abstract]

"Negative regulation of protein phosphatase 2Cbeta by ISG15 conjugation."
FEBS Lett. 580:4521-4526(2006) [PubMed] [Europe PMC] [Abstract]

"Innate antiviral response targets HIV-1 release by the induction of ubiquitin-like protein ISG15."
Proc. Natl. Acad. Sci. U.S.A. 103:1440-1445(2006) [PubMed] [Europe PMC] [Abstract]

"Different roles for two ubiquitin-like domains of ISG15 in protein modification."
J. Biol. Chem. 283:13370-13377(2008) [PubMed] [Europe PMC] [Abstract]

"Nitrosylation of ISG15 prevents the disulfide bond-mediated dimerization of ISG15 and contributes to effective ISGylation."
J. Biol. Chem. 283:24484-24488(2008) [PubMed] [Europe PMC] [Abstract]

"ISG15 inhibits Ebola VP40 VLP budding in an L-domain-dependent manner by blocking Nedd4 ligase activity."
Proc. Natl. Acad. Sci. U.S.A. 105:3974-3979(2008) [PubMed] [Europe PMC] [Abstract]

"ISG15 modification of filamin B negatively regulates the type I interferon-induced JNK signalling pathway."
EMBO Rep. 10:374-380(2009) [PubMed] [Europe PMC] [Abstract]

"Antiviral activity of innate immune protein ISG15."
J. Innate Immun. 1:397-404(2009) [PubMed] [Europe PMC] [Abstract]

"Interferon-induced ISG15 conjugation inhibits influenza A virus gene expression and replication in human cells."
J. Virol. 83:5971-5977(2009) [PubMed] [Europe PMC] [Abstract]

"ISG15 and immune diseases."
Biochim. Biophys. Acta 1802:485-496(2010) [PubMed] [Europe PMC] [Abstract]

"Emerging role of ISG15 in antiviral immunity."
Cell 143:187-190(2010) [PubMed] [Europe PMC] [Abstract]

"The interferon stimulated gene 15 functions as a proviral factor for the hepatitis C virus and as a regulator of the IFN response."
Gut 59:1111-1119(2010) [PubMed] [Europe PMC] [Abstract]

"Positive regulation of interferon regulatory factor 3 activation by Herc5 via ISG15 modification."
Mol. Cell. Biol. 30:2424-2436(2010) [PubMed] [Europe PMC] [Abstract]

"ISG15 conjugation system targets the viral NS1 protein in influenza A virus-infected cells."
Proc. Natl. Acad. Sci. U.S.A. 107:2253-2258(2010) [PubMed] [Europe PMC] [Abstract]

"Antiviral properties of ISG15."
Viruses 2:2154-2168(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Interferon-stimulated gene 15 and the protein ISGylation system."
J. Interferon Cytokine Res. 31:119-130(2011) [PubMed] [Europe PMC] [Abstract]

"Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15."
J. Virol. 85:7153-7161(2011) [PubMed] [Europe PMC] [Abstract]

"Budding of enveloped viruses: interferon-induced ISG15-antivirus mechanisms targeting the release process."
Adv. Virol. 2012:532723-532723(2012) [PubMed] [Europe PMC] [Abstract]

"IFNs, ISGylation and cancer: Cui prodest?"
Cytokine Growth Factor Rev. 23:307-314(2012) [PubMed] [Europe PMC] [Abstract]

"Covalent protein modification with ISG15 via a conserved cysteine in the hinge region."
PLoS ONE 7:E38294-E38294(2012) [PubMed] [Europe PMC] [Abstract]

"Mycobacterial disease and impaired IFN-gamma immunity in humans with inherited ISG15 deficiency."
Science 337:1684-1688(2012) [PubMed] [Europe PMC] [Abstract]

"ISG15 regulates IFN-? immunity in human mycobacterial disease."
Cell Res. 23:173-175(2013) [PubMed] [Europe PMC] [Abstract]

"ISG15: leading a double life as a secreted molecule."
Exp. Mol. Med. 45:E18-E18(2013) [PubMed] [Europe PMC] [Abstract]

"Activation of double-stranded RNA-activated protein kinase (PKR) by interferon-stimulated gene 15 (ISG15) modification down-regulates protein translation."
J. Biol. Chem. 288:2839-2847(2013) [PubMed] [Europe PMC] [Abstract]

"Interferon-induced ISG15 pathway: an ongoing virus-host battle."
Trends Microbiol. 21:181-186(2013) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of the interferon-induced ubiquitin-like protein ISG15."
J. Biol. Chem. 280:27356-27365(2005) [PubMed] [Europe PMC] [Abstract]

"Solution NMR structure of the C-terminal domain of the interferon alpha-inducible ISG15 protein from Homo sapiens."
Submitted (FEB-2009) to the PDB data bank