PDZ And LIM Domain Protein 1 (PDLIM1)

CLIM1; CLP-36; CLP36; ELFIN; hCLIM1; Carboxyl Terminal LIM Domain Protein 1; C-terminal LIM domain protein 1; LIM domain protein CLP-36

SPN	M397
HGNC	2067
MGI	1860611
PubMed	19780892
RGD	68324
UniProt	O00151
Wiki	PDLIM1

The LIM domain of PDLIM1 interacts with the C-terminal EF-hand region of alpha-actinin-2 (ACTN2). Immunoprecipitation, Western blot analysis, and immunofluorescence microscopy demonstrated that the 36-kD PDLIM1 protein colocalizes with ACTN2 in the Z discs of myocardial sarcomeres, particularly at intercalated discs, and with vinculin (VCL), which is localized in the fascia adherens of intercalated discs.

Sequence analysis predicted that the 329-amino acid PDLIM1 protein, which is 88% homologous to the rat protein, shares 51 to 61% identity with the PDZ domains of ENIGMA, ALP, ENH, and RIL. SDS-PAGE analysis showed that recombinant PDLIM1 was expressed as a 36-kD protein.

Reference

"Characterization of the human 36-kDa carboxyl terminal LIM domain protein (hCLIM1)."
J. Cell. Biochem. 72:279-285(1999) [PubMed] [Europe PMC] [Abstract]

"Human CLP-36, a PDZ-domain and LIM-domain protein, binds to alpha-actinin-1 and associates with actin filaments and stress fibers in activated platelets and endothelial cells."
Blood 96:4236-4245(2000) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and comparative analysis of human chromosome 10."
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."
Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]

, Submitted (NOV-2005) to UniProtKB

"Interaction of hCLIM1, an enigma family protein, with alpha-actinin 2."
J. Cell. Biochem. 78:558-565(2000) [PubMed] [Europe PMC] [Abstract]

"CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4."
J. Biol. Chem. 275:11100-11105(2000) [PubMed] [Europe PMC] [Abstract]

"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]

"Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]

"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]

"Phosphoproteome of resting human platelets."
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Solution structure of the LIM domain of carboxyl terminal LIM domain protein 1."
Submitted (NOV-2005) to the PDB data bank