Specificity Protein 1 (Sp1)

TSFP1; Sp1 Transcription Factor

SPN	M133
HGNC	11205
MGI	98372
PubMed	23250796
RGD	3738
UniProt	P08047
Wiki	SP1

Sp1 is a human transcription factor involved in gene expression in the early development of an organism. It belongs to the Sp/KLF family of transcription factors. The protein is 785 amino acids long, with a molecular weight of 81 kDA. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys₂/His₂ type and binds the consensus sequence 5'-(G/T)GGGCGG(G/A)(G/A)(C/T)-3' (GC box element). It has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain.Sp1 has been used as a control protein to compare with when studying the increase or decrease of the aryl hydrocarbon receptor and/or the estrogen receptor, since it binds to both and generally remains at a relatively constant level.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM133Hu01	Recombinant Specificity Protein 1 (Sp1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM133Hu01	Monoclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IP.
Antibodies	PAM133Hu01	Polyclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IF.
Assay Kits	CLM133Hu01	CLIA Kit for Specificity Protein 1 (Sp1)	CLIA assay for Antigen Detection
Assay Kits	ELM133Hu01	ELISA Kit for Specificity Protein 1 (Sp1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM133Mu01	Recombinant Specificity Protein 1 (Sp1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM133Mu01	Monoclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IP.
Antibodies	PAM133Mu01	Polyclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IF.
Assay Kits	CLM133Mu01	CLIA Kit for Specificity Protein 1 (Sp1)	CLIA assay for Antigen Detection
Assay Kits	ELM133Mu01	ELISA Kit for Specificity Protein 1 (Sp1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM133Ra01	Recombinant Specificity Protein 1 (Sp1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM133Ra01	Monoclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IP.
Antibodies	PAM133Ra01	Polyclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IF.
Assay Kits	CLM133Ra01	CLIA Kit for Specificity Protein 1 (Sp1)	CLIA assay for Antigen Detection
Assay Kits	ELM133Ra01	ELISA Kit for Specificity Protein 1 (Sp1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM133Ca01	Recombinant Specificity Protein 1 (Sp1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM133Ca01	Monoclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IP.
Antibodies	PAM133Ca01	Polyclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IF.
Assay Kits	CLM133Ca01	CLIA Kit for Specificity Protein 1 (Sp1)	CLIA assay for Antigen Detection
Assay Kits	ELM133Ca01	ELISA Kit for Specificity Protein 1 (Sp1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM133Po01	Recombinant Specificity Protein 1 (Sp1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM133Po01	Monoclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IP.
Antibodies	PAM133Po01	Polyclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IF.
Assay Kits	CLM133Po01	CLIA Kit for Specificity Protein 1 (Sp1)	CLIA assay for Antigen Detection
Assay Kits	ELM133Po01	ELISA Kit for Specificity Protein 1 (Sp1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM133Bo01	Recombinant Specificity Protein 1 (Sp1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM133Bo01	Monoclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IP.
Antibodies	PAM133Bo01	Polyclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IF.
Assay Kits	CLM133Bo01	CLIA Kit for Specificity Protein 1 (Sp1)	CLIA assay for Antigen Detection
Assay Kits	ELM133Bo01	ELISA Kit for Specificity Protein 1 (Sp1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM133Rb01	Recombinant Specificity Protein 1 (Sp1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM133Rb01	Monoclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IP.
Antibodies	PAM133Rb01	Polyclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IF.
Assay Kits	CLM133Rb01	CLIA Kit for Specificity Protein 1 (Sp1)	CLIA assay for Antigen Detection
Assay Kits	ELM133Rb01	ELISA Kit for Specificity Protein 1 (Sp1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPM133Mo01	Recombinant Specificity Protein 1 (Sp1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAM133Mo01	Monoclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IP.
Antibodies	PAM133Mo01	Polyclonal Antibody to Specificity Protein 1 (Sp1)	WB; IHC; ICC; IF.
Assay Kits	CLM133Mo01	CLIA Kit for Specificity Protein 1 (Sp1)	CLIA assay for Antigen Detection
Assay Kits	ELM133Mo01	ELISA Kit for Specificity Protein 1 (Sp1)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Identification of a novel Sp1 splice variant as a strong transcriptional activator."
Biochem. Biophys. Res. Commun. 412:86-91(2011) [PubMed] [Europe PMC] [Abstract]

"The finished DNA sequence of human chromosome 12."
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Heterogeneous Sp1 mRNAs in human HepG2 cells include a product of homotypic trans-splicing."
J. Biol. Chem. 275:38067-38072(2000) [PubMed] [Europe PMC] [Abstract]

"Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain."
Cell 51:1079-1090(1987) [PubMed] [Europe PMC] [Abstract]

"O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation."
Cell 55:125-133(1988) [PubMed] [Europe PMC] [Abstract]

"Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif."
Cell 55:887-898(1988) [PubMed] [Europe PMC] [Abstract]

"Interaction of virion protein Vpr of human immunodeficiency virus type 1 with cellular transcription factor Sp1 and trans-activation of viral long terminal repeat."
J. Biol. Chem. 270:25564-25569(1995) [PubMed] [Europe PMC] [Abstract]

"The serotonin 1a receptor gene contains a TATA-less promoter that responds to MAZ and Sp1."
J. Biol. Chem. 271:4417-4430(1996) [PubMed] [Europe PMC] [Abstract]

"O glycosylation of an Sp1-derived peptide blocks known Sp1 protein interactions."
Mol. Cell. Biol. 17:6472-6480(1997) [PubMed] [Europe PMC] [Abstract]

"The SV40 capsid protein VP3 cooperates with the cellular transcription factor Sp1 in DNA-binding and in regulating viral promoter activity."
J. Mol. Biol. 275:187-195(1998) [PubMed] [Europe PMC] [Abstract]

"Functional interactions between Sp1 or Sp3 and the helicase-like transcription factor mediate basal expression from the human plasminogen activator inhibitor-1 gene."
J. Biol. Chem. 274:19573-19580(1999) [PubMed] [Europe PMC] [Abstract]

"A set of proteins interacting with transcription factor Sp1 identified in a two-hybrid screening."
Mol. Cell. Biochem. 210:131-142(2000) [PubMed] [Europe PMC] [Abstract]

"O-linkage of N-acetylglucosamine to Sp1 activation domain inhibits its transcriptional capability."
Proc. Natl. Acad. Sci. U.S.A. 98:6611-6616(2001) [PubMed] [Europe PMC] [Abstract]

"Identification of two Sp1 phosphorylation sites for p42/p44 mitogen-activated protein kinases: their implication in vascular endothelial growth factor gene transcription."
J. Biol. Chem. 277:20631-20639(2002) [PubMed] [Europe PMC] [Abstract]

"Cellular transcription factor Sp1 recruits simian virus 40 capsid proteins to the viral packaging signal, ses."
J. Virol. 76:5915-5924(2002) [PubMed] [Europe PMC] [Abstract]

"Che-1 arrests human colon carcinoma cell proliferation by displacing HDAC1 from the p21WAF1/CIP1 promoter."
J. Biol. Chem. 278:36496-36504(2003) [PubMed] [Europe PMC] [Abstract]

"Interaction between the varicella zoster virus IE62 major transactivator and cellular transcription factor Sp1."
J. Biol. Chem. 278:38068-38075(2003) [PubMed] [Europe PMC] [Abstract]

"Fibroblast growth factor-2 represses platelet-derived growth factor receptor-alpha (PDGFR-alpha) transcription via ERK1/2-dependent Sp1 phosphorylation and an atypical cis-acting element in the proximal PDGFR-alpha promoter."
J. Biol. Chem. 279:2377-2382(2004) [PubMed] [Europe PMC] [Abstract]

"Transcriptional repression and heterochromatin formation by MBD1 and MCAF/AM family proteins."
J. Biol. Chem. 280:13928-13935(2005) [PubMed] [Europe PMC] [Abstract]

"Insulin dynamically regulates calmodulin gene expression by sequential O-glycosylation and phosphorylation of SP1 and its subcellular compartmentalization in liver cells."
J. Biol. Chem. 281:3642-3650(2006) [PubMed] [Europe PMC] [Abstract]

"HER-2/neu represses the metastasis suppressor RECK via ERK and Sp transcription factors to promote cell invasion."
J. Biol. Chem. 281:4718-4725(2006) [PubMed] [Europe PMC] [Abstract]

"Sumoylation inhibits cleavage of Sp1 N-terminal negative regulatory domain and inhibits Sp1-dependent transcription."
J. Biol. Chem. 281:5567-5574(2006) [PubMed] [Europe PMC] [Abstract]

"Increased chromatin association of Sp1 in interphase cells by PP2A-mediated dephosphorylations."
J. Mol. Biol. 364:897-908(2006) [PubMed] [Europe PMC] [Abstract]

"Sp1 deacetylation induced by phorbol ester recruits p300 to activate 12(S)-lipoxygenase gene transcription."
Mol. Cell. Biol. 26:1770-1785(2006) [PubMed] [Europe PMC] [Abstract]

"Phosphatidylinositol 3-kinase/protein kinase Czeta-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated derepression of transcription of the luteinizing hormone receptor gene."
Mol. Cell. Biol. 26:6748-6761(2006) [PubMed] [Europe PMC] [Abstract]

Erratum
Mol. Cell. Biol. 26:8214-8214(2006)

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation of Sp1 in response to DNA damage by ataxia telangiectasia-mutated kinase."
Mol. Cancer Res. 5:1319-1330(2007) [PubMed] [Europe PMC] [Abstract]

"Activation of PPARgamma negatively regulates O-GlcNAcylation of Sp1."
Biochem. Biophys. Res. Commun. 372:713-718(2008) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation mediates Sp1 coupled activities of proteolytic processing, desumoylation and degradation."
Cell Cycle 7:623-630(2008) [PubMed] [Europe PMC] [Abstract]

"Identification of phosphorylation sites on transcription factor Sp1 in response to DNA damage and its accumulation at damaged sites."
Cell. Signal. 20:1795-1803(2008) [PubMed] [Europe PMC] [Abstract]

"Angiotensin II-inducible platelet-derived growth factor-D transcription requires specific Ser/Thr residues in the second zinc finger region of Sp1."
Circ. Res. 102:38-51(2008) [PubMed] [Europe PMC] [Abstract]

"Phosphorylation by c-Jun NH2-terminal kinase 1 regulates the stability of transcription factor Sp1 during mitosis."
Mol. Biol. Cell 19:1139-1151(2008) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"O-GlcNAc inhibits interaction between Sp1 and Elf-1 transcription factors."
Biochem. Biophys. Res. Commun. 380:569-574(2009) [PubMed] [Europe PMC] [Abstract]

"O-GlcNAcylation of Sp1 interrupts Sp1 interaction with NF-Y."
Biochem. Biophys. Res. Commun. 382:593-597(2009) [PubMed] [Europe PMC] [Abstract]

"MCAF1/AM is involved in Sp1-mediated maintenance of cancer-associated telomerase activity."
J. Biol. Chem. 284:5165-5174(2009) [PubMed] [Europe PMC] [Abstract]

"O-linked N-acetylglucosaminylation of Sp1 inhibits the human immunodeficiency virus type 1 promoter."
J. Virol. 83:3704-3718(2009) [PubMed] [Europe PMC] [Abstract]

"Human BAHD1 promotes heterochromatic gene silencing."
Proc. Natl. Acad. Sci. U.S.A. 106:13826-13831(2009) [PubMed] [Europe PMC] [Abstract]

"Snail associates with EGR-1 and SP-1 to upregulate transcriptional activation of p15INK4b."
FEBS J. 277:1202-1218(2010) [PubMed] [Europe PMC] [Abstract]

"Transcriptional regulation of human FE65, a ligand of Alzheimer's disease amyloid precursor protein, by Sp1."
J. Cell. Biochem. 109:782-793(2010) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Transforming growth factor-beta-inducible early response gene 1 is a novel substrate for atypical protein kinase Cs."
Cell. Mol. Life Sci. 68:1953-1968(2011) [PubMed] [Europe PMC] [Abstract]

"Cooperative transcriptional activation by Klf4, Meis2, and Pbx1."
Mol. Cell. Biol. 31:3723-3733(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"Structures of zinc finger domains from transcription factor Sp1. Insights into sequence-specific protein-DNA recognition."
J. Biol. Chem. 272:7801-7809(1997) [PubMed] [Europe PMC] [Abstract]