Rh Associated Glycoprotein (RHAG)

CD241; RH2; RH50A; Rh50 GP; SLC42A1; Ammonium Transporter Rh Type A; Rhesus Blood Group-Associated Glycoprotein; Erythrocyte plasma membrane 50 kDa glycoprotein

SPN	V121
HGNC	10006
MGI	1202713
PubMed	10607721
RGD	61871
UniProt	Q02094
Wiki	RHAG

Ridgwell et al. (1992) isolated cDNA clones representing a member of the Rh50 glycoprotein family, the Rh50A glycoprotein. They used PCR with degenerate primers based on the N-terminal amino acid sequence of the Rh50 glycoproteins and human genomic DNA as a template. The cDNA clones containing the full coding sequence of the Rh50A glycoprotein predicted a 409-amino acid N-glycosylated membrane protein with up to 12 transmembrane domains.

It showed clear similarity to the Rh30A protein in both amino acid sequence and predicted topology. The findings were considered consistent with the possibility that the Rh30 and Rh50 groups of proteins are different subunits of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.

Reference

"Isolation of cDNA clones for a 50 kDa glycoprotein of the human erythrocyte membrane associated with Rh (rhesus) blood-group antigen expression."
Biochem. J. 287:223-228(1992) [PubMed] [Europe PMC] [Abstract]

"The human Rh50 glycoprotein gene. Structural organization and associated splicing defect resulting in Rh(null) disease."
J. Biol. Chem. 273:2207-2213(1998) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and analysis of human chromosome 6."
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]

"Identification of 5' flanking sequence of RH50 gene and the core region for erythroid-specific expression."
Biochem. Biophys. Res. Commun. 243:233-240(1998) [PubMed] [Europe PMC] [Abstract]

"Protein-sequence studies on Rh-related polypeptides suggest the presence of at least two groups of proteins which associate in the human red-cell membrane."
Biochem. J. 256:1043-1046(1988) [PubMed] [Europe PMC] [Abstract]

"The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast."
Nat. Genet. 26:341-344(2000) [PubMed] [Europe PMC] [Abstract]

"Identification of the erythrocyte Rh blood group glycoprotein as a mammalian ammonium transporter."
J. Biol. Chem. 277:12499-12502(2002) [PubMed] [Europe PMC] [Abstract]

"Mechanism of genetic complementation of ammonium transport in yeast by human erythrocyte Rh-associated glycoprotein."
J. Biol. Chem. 279:17443-17448(2004) [PubMed] [Europe PMC] [Abstract]

"Candidate gene acting as a suppressor of the RH locus in most cases of Rh-deficiency."
Nat. Genet. 12:168-173(1996) [PubMed] [Europe PMC] [Abstract]

"A novel single missense mutation identified along the RH50 gene in a composite heterozygous Rhnull blood donor of the regulator type."
Blood 91:1458-1463(1998) [PubMed] [Europe PMC] [Abstract]

"Rh50 glycoprotein gene and rhnull disease: a silent splice donor is trans to a Gly279-->Glu missense mutation in the conserved transmembrane segment."
Blood 92:1776-1784(1998) [PubMed] [Europe PMC] [Abstract]

"Molecular basis for Rh(null) syndrome: identification of three new missense mutations in the Rh50 glycoprotein gene."
Am. J. Hematol. 62:25-32(1999) [PubMed] [Europe PMC] [Abstract]