Polyclonal Antibody to Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus) Histone H3 (H3)

Description: Histone H3 (H3)

Immunogen: W477Mi01-Recombinant Histone H3 (H3)

Source:Polyclonal antibody preparation

Host: Rabbit

Reactivity: Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Rhesus monkey (Simian), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus)

Isotype: IgG

Purification: Antigen-specific affinity chromatography

Label: None

Buffer Formulation: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Form Supplied: Liquid

Concentration: 200ug/ml

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

 

Specifity: The antibody is a rabbit Polyclonal antibody raised against H3. It has been selected for its ability to recognize H3 in immunohistochemical staining and western blotting.

Western Blot: 

Sample: Recombinant Human H3 
Anti-Human H3 antibody was used at 1:400 dilution.

 

 

 

Immunohistochemical staining:

 

DAB staining on IHC-P Samples:Tissue.

 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.