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Monoclonal Antibody to Rattus norvegicus (Rat) Nephrin (NPHN)

NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor

Description: Nephrin (NPHN)

Immunogen: V874Ra01-Recombinant Nephrin (NPHN)

Source:Monoclonal antibody preparation

Host: Mouse

Reactivity: Rattus norvegicus (Rat)

Isotype: IgG

Purification: Antigen-specific affinity chromatography

Label: None

Buffer Formulation: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Form Supplied: Liquid

Concentration: 200ug/ml

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

 

Specifity: The antibody is a rabbit Polyclonal antibody raised against NPHN. It has been selected for its ability to recognize NPHN in immunohistochemical staining and western blotting.

Western Blot: 
Sample: Recombinant Rat NPHN 
Anti-Rat NPHN antibody was used at 1:400 dilution.

 

 

 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

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V874Mu02 Nephrin (NPHN) Recombinant Protein Mus musculus (Mouse)
V874Ra01 Nephrin (NPHN) Recombinant Protein Rattus norvegicus (Rat)