Ribonuclease H (RNASEH)
Rnase-H; RNASEH1; RNH1; Ribonuclease H type II
The enzyme RNase H is a ribonuclease that cleaves the 3'-O-P-bond of RNA in a DNA/RNA duplex to produce 3'-hydroxyl and 5'-phosphate terminated products. RNase H is a non-specific endonuclease and catalyzes the cleavage of RNA via an hydrolytic mechanism, aided by an enzyme-bound divalent metal ion.
Members of the RNase H family can be found in nearly all organisms, from archaea and prokaryota to eukaryota. In DNA replication, RNase H is responsible for cutting out the RNA primer, allowing completion of the newly synthesized DNA.
Retroviral RNase H, a part of the viral reverse transcriptase enzyme, is an important pharmaceutical target, as it is absolutely necessary for the proliferation of retroviruses, such as HIV.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW540Hu01 | Recombinant Ribonuclease H (RNASEH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW540Hu01 | Monoclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IP. |
| PAW540Hu01 | Polyclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW540Hu01 | CLIA Kit for Ribonuclease H (RNASEH) | CLIA assay for Antigen Detection |
| ELW540Hu01 | ELISA Kit for Ribonuclease H (RNASEH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW540Mu01 | Recombinant Ribonuclease H (RNASEH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW540Mu01 | Monoclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IP. |
| PAW540Mu01 | Polyclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW540Mu01 | CLIA Kit for Ribonuclease H (RNASEH) | CLIA assay for Antigen Detection |
| ELW540Mu01 | ELISA Kit for Ribonuclease H (RNASEH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW540Ra01 | Recombinant Ribonuclease H (RNASEH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW540Ra01 | Monoclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IP. |
| PAW540Ra01 | Polyclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW540Ra01 | CLIA Kit for Ribonuclease H (RNASEH) | CLIA assay for Antigen Detection |
| ELW540Ra01 | ELISA Kit for Ribonuclease H (RNASEH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW540Ca01 | Recombinant Ribonuclease H (RNASEH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW540Ca01 | Monoclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IP. |
| PAW540Ca01 | Polyclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW540Ca01 | CLIA Kit for Ribonuclease H (RNASEH) | CLIA assay for Antigen Detection |
| ELW540Ca01 | ELISA Kit for Ribonuclease H (RNASEH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW540Po01 | Recombinant Ribonuclease H (RNASEH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW540Po01 | Monoclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IP. |
| PAW540Po01 | Polyclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW540Po01 | CLIA Kit for Ribonuclease H (RNASEH) | CLIA assay for Antigen Detection |
| ELW540Po01 | ELISA Kit for Ribonuclease H (RNASEH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW540Bo01 | Recombinant Ribonuclease H (RNASEH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW540Bo01 | Monoclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IP. |
| PAW540Bo01 | Polyclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW540Bo01 | CLIA Kit for Ribonuclease H (RNASEH) | CLIA assay for Antigen Detection |
| ELW540Bo01 | ELISA Kit for Ribonuclease H (RNASEH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW540Rb01 | Recombinant Ribonuclease H (RNASEH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW540Rb01 | Monoclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IP. |
| PAW540Rb01 | Polyclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW540Rb01 | CLIA Kit for Ribonuclease H (RNASEH) | CLIA assay for Antigen Detection |
| ELW540Rb01 | ELISA Kit for Ribonuclease H (RNASEH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW540Mo01 | Recombinant Ribonuclease H (RNASEH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW540Mo01 | Monoclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IP. |
| PAW540Mo01 | Polyclonal Antibody to Ribonuclease H (RNASEH) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW540Mo01 | CLIA Kit for Ribonuclease H (RNASEH) | CLIA assay for Antigen Detection |
| ELW540Mo01 | ELISA Kit for Ribonuclease H (RNASEH) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI."
Genomics 53:300-307(1998) [PubMed] [Europe PMC] [Abstract]
"Molecular cloning and expression of cDNA for human RNase H."
Antisense Nucleic Acid Drug Dev. 8:53-61(1998) [PubMed] [Europe PMC] [Abstract]
"Cloning, subcellular localization and functional expression of human RNase HII."
Biol. Chem. 379:1407-1412(1998) [PubMed] [Europe PMC] [Abstract]
"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Properties of cloned and expressed human RNase H1."
J. Biol. Chem. 274:28270-28278(1999) [PubMed] [Europe PMC] [Abstract]
"Human senataxin resolves RNA/DNA hybrids formed at transcriptional pause sites to promote Xrn2-dependent termination."
Mol. Cell 42:794-805(2011) [PubMed] [Europe PMC] [Abstract]
"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]