Linker For Activation Of T-Cell (LAT)
LAT1; Pp36; Linker Of Activated T Cells; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein
Linker of Activated T cells also known as LAT is a protein which is encoded by the LAT gene. Alternative splicing results in multiple transcript variants encoding different isoforms.
The protein encoded by this gene is phosphorylated by ZAP70/SYK protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts (also known as glycosphingolipid-enriched microdomains or GEMs) and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW490Hu01 | Recombinant Linker For Activation Of T-Cell (LAT) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW490Hu01 | Monoclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IP. |
| PAW490Hu01 | Polyclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW490Hu01 | CLIA Kit for Linker For Activation Of T-Cell (LAT) | CLIA assay for Antigen Detection |
| ELW490Hu01 | ELISA Kit for Linker For Activation Of T-Cell (LAT) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW490Mu01 | Recombinant Linker For Activation Of T-Cell (LAT) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW490Mu01 | Monoclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IP. |
| PAW490Mu01 | Polyclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW490Mu01 | CLIA Kit for Linker For Activation Of T-Cell (LAT) | CLIA assay for Antigen Detection |
| ELW490Mu01 | ELISA Kit for Linker For Activation Of T-Cell (LAT) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW490Ra01 | Recombinant Linker For Activation Of T-Cell (LAT) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW490Ra01 | Monoclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IP. |
| PAW490Ra01 | Polyclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW490Ra01 | CLIA Kit for Linker For Activation Of T-Cell (LAT) | CLIA assay for Antigen Detection |
| ELW490Ra01 | ELISA Kit for Linker For Activation Of T-Cell (LAT) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW490Ca01 | Recombinant Linker For Activation Of T-Cell (LAT) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW490Ca01 | Monoclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IP. |
| PAW490Ca01 | Polyclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW490Ca01 | CLIA Kit for Linker For Activation Of T-Cell (LAT) | CLIA assay for Antigen Detection |
| ELW490Ca01 | ELISA Kit for Linker For Activation Of T-Cell (LAT) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW490Po01 | Recombinant Linker For Activation Of T-Cell (LAT) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW490Po01 | Monoclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IP. |
| PAW490Po01 | Polyclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW490Po01 | CLIA Kit for Linker For Activation Of T-Cell (LAT) | CLIA assay for Antigen Detection |
| ELW490Po01 | ELISA Kit for Linker For Activation Of T-Cell (LAT) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW490Bo01 | Recombinant Linker For Activation Of T-Cell (LAT) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW490Bo01 | Monoclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IP. |
| PAW490Bo01 | Polyclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW490Bo01 | CLIA Kit for Linker For Activation Of T-Cell (LAT) | CLIA assay for Antigen Detection |
| ELW490Bo01 | ELISA Kit for Linker For Activation Of T-Cell (LAT) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW490Rb01 | Recombinant Linker For Activation Of T-Cell (LAT) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW490Rb01 | Monoclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IP. |
| PAW490Rb01 | Polyclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW490Rb01 | CLIA Kit for Linker For Activation Of T-Cell (LAT) | CLIA assay for Antigen Detection |
| ELW490Rb01 | ELISA Kit for Linker For Activation Of T-Cell (LAT) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW490Mo01 | Recombinant Linker For Activation Of T-Cell (LAT) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW490Mo01 | Monoclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IP. |
| PAW490Mo01 | Polyclonal Antibody to Linker For Activation Of T-Cell (LAT) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW490Mo01 | CLIA Kit for Linker For Activation Of T-Cell (LAT) | CLIA assay for Antigen Detection |
| ELW490Mo01 | ELISA Kit for Linker For Activation Of T-Cell (LAT) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation."
Cell 92:83-92(1998) [PubMed] [Europe PMC] [Abstract]
"Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells."
J. Exp. Med. 187:1157-1161(1998) [PubMed] [Europe PMC] [Abstract]
"The sequence and analysis of duplication-rich human chromosome 16."
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"LAT palmitoylation: its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation."
Immunity 9:239-246(1998) [PubMed] [Europe PMC] [Abstract]
"Requirement of the Src homology 2 domain protein Shb for T cell receptor-dependent activation of the interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells."
J. Biol. Chem. 274:28050-28057(1999) [PubMed] [Europe PMC] [Abstract]
"A role for the adaptor protein LAT in human NK cell-mediated cytotoxicity."
J. Immunol. 162:2453-2456(1999) [PubMed] [Europe PMC] [Abstract]
"Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell antigen receptor-mediated signaling."
J. Biol. Chem. 275:23355-23361(2000) [PubMed] [Europe PMC] [Abstract]
"The adapter protein LAT enhances fcgamma receptor-mediated signal transduction in myeloid cells."
J. Biol. Chem. 275:20480-20487(2000) [PubMed] [Europe PMC] [Abstract]
"Interaction of linker for activation of T cells with multiple adapter proteins in platelets activated by the glycoprotein VI-selective ligand, convulxin."
J. Biol. Chem. 275:33427-33434(2000) [PubMed] [Europe PMC] [Abstract]
"Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells."
Biochem. J. 356:461-471(2001) [PubMed] [Europe PMC] [Abstract]
"Protein tyrosine phosphatase CD148-mediated inhibition of T-cell receptor signal transduction is associated with reduced LAT and phospholipase Cgamma1 phosphorylation."
Mol. Cell. Biol. 21:2393-2403(2001) [PubMed] [Europe PMC] [Abstract]
"Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav."
Biochemistry 41:10732-10740(2002) [PubMed] [Europe PMC] [Abstract]
"The tyrosine phosphatase CD148 is excluded from the immunologic synapse and down-regulates prolonged T cell signaling."
J. Cell Biol. 162:673-682(2003) [PubMed] [Europe PMC] [Abstract]
"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."
Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
"LAT: a T lymphocyte adapter protein that couples the antigen receptor to downstream signaling pathways."
Bioessays 26:61-67(2004) [PubMed] [Europe PMC] [Abstract]
"Transmembrane adaptor molecules: a new category of lymphoid-cell markers."
Blood 107:213-221(2006) [PubMed] [Europe PMC] [Abstract]
"Phosphoproteome of resting human platelets."
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]