Alpha-Hemoglobin Stabilizing Protein (aHSP)
A-HSP; ERAF; EDRF; Erythroid Associated Factor; Erythroid differentiation-related factor
High pressure induces an irreversible aggregation of the ferrous deoxy α-chains, whereas the AHSP/α-Hb complex shows reversible hexacoordination of the α-Hb without protein aggregation. Upon pressure release, the relaxation kinetics of the transition from the hexacoordinated to pentacoordinated form of α-Hb in the presence of AHSP exhibit a biphasic shape. High pressure did not induce dissociation of α-Hb from its chaperone, as evidenced by the ligand binding kinetics that show a unique rate for the AHSP/α-Hb complex. For both free α-Hb and the AHSP/α-Hb complex, the bimolecular rate constant of CO binding (kCOon) versus pressure exhibits a bell shape, attributed to the transition of the rate-determining step from the chemical barrier to the migration of CO within the protein matrix.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW393Hu01 | Recombinant Alpha-Hemoglobin Stabilizing Protein (aHSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW393Hu01 | Monoclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IP. |
| PAW393Hu01 | Polyclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW393Hu01 | CLIA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | CLIA assay for Antigen Detection |
| ELW393Hu01 | ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW393Mu01 | Recombinant Alpha-Hemoglobin Stabilizing Protein (aHSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW393Mu01 | Monoclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IP. |
| PAW393Mu01 | Polyclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW393Mu01 | CLIA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | CLIA assay for Antigen Detection |
| ELW393Mu01 | ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW393Ra01 | Recombinant Alpha-Hemoglobin Stabilizing Protein (aHSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW393Ra01 | Monoclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IP. |
| PAW393Ra01 | Polyclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW393Ra01 | CLIA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | CLIA assay for Antigen Detection |
| ELW393Ra01 | ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW393Ca01 | Recombinant Alpha-Hemoglobin Stabilizing Protein (aHSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW393Ca01 | Monoclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IP. |
| PAW393Ca01 | Polyclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW393Ca01 | CLIA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | CLIA assay for Antigen Detection |
| ELW393Ca01 | ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW393Po01 | Recombinant Alpha-Hemoglobin Stabilizing Protein (aHSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW393Po01 | Monoclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IP. |
| PAW393Po01 | Polyclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW393Po01 | CLIA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | CLIA assay for Antigen Detection |
| ELW393Po01 | ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW393Bo01 | Recombinant Alpha-Hemoglobin Stabilizing Protein (aHSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW393Bo01 | Monoclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IP. |
| PAW393Bo01 | Polyclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW393Bo01 | CLIA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | CLIA assay for Antigen Detection |
| ELW393Bo01 | ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW393Rb01 | Recombinant Alpha-Hemoglobin Stabilizing Protein (aHSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW393Rb01 | Monoclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IP. |
| PAW393Rb01 | Polyclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW393Rb01 | CLIA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | CLIA assay for Antigen Detection |
| ELW393Rb01 | ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW393Mo01 | Recombinant Alpha-Hemoglobin Stabilizing Protein (aHSP) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW393Mo01 | Monoclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IP. |
| PAW393Mo01 | Polyclonal Antibody to Alpha-Hemoglobin Stabilizing Protein (aHSP) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW393Mo01 | CLIA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | CLIA assay for Antigen Detection |
| ELW393Mo01 | ELISA Kit for Alpha-Hemoglobin Stabilizing Protein (aHSP) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
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Nature 417:758-763(2002) [PubMed] [Europe PMC] [Abstract]
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J. Biol. Chem. 277:40602-40609(2002) [PubMed] [Europe PMC] [Abstract]
"Molecular mechanism of AHSP-mediated stabilization of alpha-hemoglobin."
Cell 119:629-640(2004) [PubMed] [Europe PMC] [Abstract]
"NMR structure of the alpha-hemoglobin stabilizing protein: insights into conformational heterogeneity and binding."
J. Biol. Chem. 279:34963-34970(2004) [PubMed] [Europe PMC] [Abstract]
"Structure of oxidized alpha-haemoglobin bound to AHSP reveals a protective mechanism for haem."
Nature 435:697-701(2005) [PubMed] [Europe PMC] [Abstract]