Topoisomerase II (TOP2)

DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme

SPN	W020
HGNC	11989
MGI	98790
PubMed	16101488
RGD	62048
UniProt	P11388
Wiki	TOP2

Type II topoisomerase is an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPW020Hu01	Recombinant Topoisomerase II (TOP2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAW020Hu01	Monoclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IP.
Antibodies	PAW020Hu01	Polyclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IF.
Assay Kits	CLW020Hu01	CLIA Kit for Topoisomerase II (TOP2)	CLIA assay for Antigen Detection
Assay Kits	ELW020Hu01	ELISA Kit for Topoisomerase II (TOP2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPW020Mu01	Recombinant Topoisomerase II (TOP2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAW020Mu01	Monoclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IP.
Antibodies	PAW020Mu01	Polyclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IF.
Assay Kits	CLW020Mu01	CLIA Kit for Topoisomerase II (TOP2)	CLIA assay for Antigen Detection
Assay Kits	ELW020Mu01	ELISA Kit for Topoisomerase II (TOP2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPW020Ra01	Recombinant Topoisomerase II (TOP2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAW020Ra01	Monoclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IP.
Antibodies	PAW020Ra01	Polyclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IF.
Assay Kits	CLW020Ra01	CLIA Kit for Topoisomerase II (TOP2)	CLIA assay for Antigen Detection
Assay Kits	ELW020Ra01	ELISA Kit for Topoisomerase II (TOP2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPW020Ca01	Recombinant Topoisomerase II (TOP2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAW020Ca01	Monoclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IP.
Antibodies	PAW020Ca01	Polyclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IF.
Assay Kits	CLW020Ca01	CLIA Kit for Topoisomerase II (TOP2)	CLIA assay for Antigen Detection
Assay Kits	ELW020Ca01	ELISA Kit for Topoisomerase II (TOP2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPW020Po01	Recombinant Topoisomerase II (TOP2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAW020Po01	Monoclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IP.
Antibodies	PAW020Po01	Polyclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IF.
Assay Kits	CLW020Po01	CLIA Kit for Topoisomerase II (TOP2)	CLIA assay for Antigen Detection
Assay Kits	ELW020Po01	ELISA Kit for Topoisomerase II (TOP2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPW020Bo01	Recombinant Topoisomerase II (TOP2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAW020Bo01	Monoclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IP.
Antibodies	PAW020Bo01	Polyclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IF.
Assay Kits	CLW020Bo01	CLIA Kit for Topoisomerase II (TOP2)	CLIA assay for Antigen Detection
Assay Kits	ELW020Bo01	ELISA Kit for Topoisomerase II (TOP2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPW020Rb01	Recombinant Topoisomerase II (TOP2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAW020Rb01	Monoclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IP.
Antibodies	PAW020Rb01	Polyclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IF.
Assay Kits	CLW020Rb01	CLIA Kit for Topoisomerase II (TOP2)	CLIA assay for Antigen Detection
Assay Kits	ELW020Rb01	ELISA Kit for Topoisomerase II (TOP2)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPW020Mo01	Recombinant Topoisomerase II (TOP2)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAW020Mo01	Monoclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IP.
Antibodies	PAW020Mo01	Polyclonal Antibody to Topoisomerase II (TOP2)	WB; IHC; ICC; IF.
Assay Kits	CLW020Mo01	CLIA Kit for Topoisomerase II (TOP2)	CLIA assay for Antigen Detection
Assay Kits	ELW020Mo01	ELISA Kit for Topoisomerase II (TOP2)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22."
Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988) [PubMed] [Europe PMC] [Abstract]

"Use of yeast in the study of anticancer drugs targeting DNA topoisomerases: expression of a functional recombinant human DNA topoisomerase II alpha in yeast."
Cancer Res. 53:3591-3596(1993) [PubMed] [Europe PMC] [Abstract]

"Structural organization of the human TOP2A and TOP2B genes."
Gene 221:255-266(1998) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication."
Biochim. Biophys. Acta 1444:395-406(1999) [PubMed] [Europe PMC] [Abstract]

"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]