Alkaline Sphingomyelinase (Alk-Smase)
ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase
Sphingomyelin phosphodiesterase or simply Sphingomyelinase (SMase) is a hydrolase enzyme that is involved in sphingolipid metabolism reactions. SMase is a member of the DNase I superfamily of enzymes and is responsible for breaking sphingomyelin (SM) down into phosphocholine and ceramide. The activation of SMase has been suggested as a major route for the production of ceramide in response to cellular stresses. Alk-Smase Converts sphingomyelin to ceramide. Also has phospholipase C activity toward palmitoyl lyso-phosphocholine. Does not appear to have nucleotide pyrophosphatase activity.Detected in the colon (at protein level). Expressed in the duodenum, jejunum and liver and at low levels in the ileum. Expression was very low in the esophagus, stomach and colon.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW011Hu01 | Recombinant Alkaline Sphingomyelinase (Alk-Smase) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW011Hu01 | Monoclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IP. |
| PAW011Hu01 | Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW011Hu01 | CLIA Kit for Alkaline Sphingomyelinase (Alk-Smase) | CLIA assay for Antigen Detection |
| ELW011Hu01 | ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW011Mu01 | Recombinant Alkaline Sphingomyelinase (Alk-Smase) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW011Mu01 | Monoclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IP. |
| PAW011Mu01 | Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW011Mu01 | CLIA Kit for Alkaline Sphingomyelinase (Alk-Smase) | CLIA assay for Antigen Detection |
| ELW011Mu01 | ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW011Ra01 | Recombinant Alkaline Sphingomyelinase (Alk-Smase) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW011Ra01 | Monoclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IP. |
| PAW011Ra01 | Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW011Ra01 | CLIA Kit for Alkaline Sphingomyelinase (Alk-Smase) | CLIA assay for Antigen Detection |
| ELW011Ra01 | ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW011Ca01 | Recombinant Alkaline Sphingomyelinase (Alk-Smase) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW011Ca01 | Monoclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IP. |
| PAW011Ca01 | Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW011Ca01 | CLIA Kit for Alkaline Sphingomyelinase (Alk-Smase) | CLIA assay for Antigen Detection |
| ELW011Ca01 | ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW011Po01 | Recombinant Alkaline Sphingomyelinase (Alk-Smase) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW011Po01 | Monoclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IP. |
| PAW011Po01 | Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW011Po01 | CLIA Kit for Alkaline Sphingomyelinase (Alk-Smase) | CLIA assay for Antigen Detection |
| ELW011Po01 | ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW011Bo01 | Recombinant Alkaline Sphingomyelinase (Alk-Smase) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW011Bo01 | Monoclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IP. |
| PAW011Bo01 | Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW011Bo01 | CLIA Kit for Alkaline Sphingomyelinase (Alk-Smase) | CLIA assay for Antigen Detection |
| ELW011Bo01 | ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW011Rb01 | Recombinant Alkaline Sphingomyelinase (Alk-Smase) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW011Rb01 | Monoclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IP. |
| PAW011Rb01 | Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW011Rb01 | CLIA Kit for Alkaline Sphingomyelinase (Alk-Smase) | CLIA assay for Antigen Detection |
| ELW011Rb01 | ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPW011Mo01 | Recombinant Alkaline Sphingomyelinase (Alk-Smase) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAW011Mo01 | Monoclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IP. |
| PAW011Mo01 | Polyclonal Antibody to Alkaline Sphingomyelinase (Alk-Smase) | WB; IHC; ICC; IF. | |
| Assay Kits | CLW011Mo01 | CLIA Kit for Alkaline Sphingomyelinase (Alk-Smase) | CLIA assay for Antigen Detection |
| ELW011Mo01 | ELISA Kit for Alkaline Sphingomyelinase (Alk-Smase) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Identification of human intestinal alkaline sphingomyelinase as a novel ecto-enzyme related to the nucleotide phosphodiesterase family."
J. Biol. Chem. 278:38528-38536(2003) [PubMed] [Europe PMC] [Abstract].
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract].
"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
"Purification, localization, and expression of human intestinal alkaline sphingomyelinase."
J. Lipid Res. 44:1241-1250(2003) [PubMed] [Europe PMC] [Abstract]
"Identification of one exon deletion of intestinal alkaline sphingomyelinase in colon cancer HT-29 cells and a differentiation-related expression of the wild-type enzyme in Caco-2 cells."
Carcinogenesis 25:1327-1333(2004) [PubMed] [Europe PMC] [Abstract]
"Functional studies of human intestinal alkaline sphingomyelinase by deglycosylation and mutagenesis."
Biochem. J. 386:153-160(2005) [PubMed] [Europe PMC] [Abstract]
"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]