Osteopontin (OPN)
SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
Osteopontin (OPN), is a secreted multifunctional glyco-phosphoprotein with roles in bone metabolism, immune regulation, tissue remodeling , cell survival, and tumor progression . Gene structure and chromosomal location identify OPN as a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family that also includes BSP,BMP1,DSPP, ENAM and MEPE. Murine OPN issynthesized as a 294 amino acid (aa) precursor protein with a predicted 16 aa signal peptide and a highly unusual mature protein sequence, containing 68 acidic aa and 23 potential Ser/Thr phosphorylation sites. Although the predicted molecular weight of OPN is 31 kDa,phosphorylation and N- and O-glycosylation may allow it to appear as large as 75 kDa. Variability in post-translational modifications can influence the activity of OPN.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV912Hu01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV912Hu01 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAV912Hu01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV912Hu01 | CLIA Kit for Osteopontin (OPN) | CLIA assay for Antigen Detection |
| ELV912Hu01 | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV912Mu01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV912Mu01 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAV912Mu01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV912Mu01 | CLIA Kit for Osteopontin (OPN) | CLIA assay for Antigen Detection |
| ELV912Mu01 | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV912Ra01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV912Ra01 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAV912Ra01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV912Ra01 | CLIA Kit for Osteopontin (OPN) | CLIA assay for Antigen Detection |
| ELV912Ra01 | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV912Ca01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV912Ca01 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAV912Ca01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV912Ca01 | CLIA Kit for Osteopontin (OPN) | CLIA assay for Antigen Detection |
| ELV912Ca01 | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV912Po01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV912Po01 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAV912Po01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV912Po01 | CLIA Kit for Osteopontin (OPN) | CLIA assay for Antigen Detection |
| ELV912Po01 | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV912Bo01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV912Bo01 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAV912Bo01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV912Bo01 | CLIA Kit for Osteopontin (OPN) | CLIA assay for Antigen Detection |
| ELV912Bo01 | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV912Rb01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV912Rb01 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAV912Rb01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV912Rb01 | CLIA Kit for Osteopontin (OPN) | CLIA assay for Antigen Detection |
| ELV912Rb01 | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV912Mo01 | Recombinant Osteopontin (OPN) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV912Mo01 | Monoclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IP. |
| PAV912Mo01 | Polyclonal Antibody to Osteopontin (OPN) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV912Mo01 | CLIA Kit for Osteopontin (OPN) | CLIA assay for Antigen Detection |
| ELV912Mo01 | ELISA Kit for Osteopontin (OPN) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"The cDNA and derived amino acid sequence for human osteopontin."
Nucleic Acids Res. 17:3306-3306(1989) [PubMed] [Europe PMC] [Abstract]
"cDNA cloning, mRNA distribution and heterogeneity, chromosomal location, and RFLP analysis of human osteopontin (OPN)."
Genomics 7:491-502(1990) [PubMed] [Europe PMC] [Abstract]
"Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily."
Proc. Natl. Acad. Sci. U.S.A. 89:426-430(1992) [PubMed] [Europe PMC] [Abstract]
"Genomic organization of the human osteopontin gene: exclusion of the locus from a causative role in the pathogenesis of dentinogenesis imperfecta type II."
Genomics 27:155-160(1995) [PubMed] [Europe PMC] [Abstract]
"Cloning and characterization of the human osteopontin gene and its promoter."
Biochem. J. 303:255-262(1994) [PubMed] [Europe PMC] [Abstract]
"Expression of osteopontin in human glioma. Its correlation with the malignancy."
Lab. Invest. 72:55-63(1995) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries."
DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Molecular cloning and sequencing of cDNA encoding urinary stone protein, which is identical to osteopontin."
Biochem. Biophys. Res. Commun. 184:859-864(1992) [PubMed] [Europe PMC] [Abstract]
"Purification of a human milk protein closely similar to tumor-secreted phosphoproteins and osteopontin."
Biochim. Biophys. Acta 996:43-48(1989) [PubMed] [Europe PMC] [Abstract]
"Post-translationally modified residues of native human osteopontin are located in clusters: identification of 36 phosphorylation and five O-glycosylation sites and their biological implications."
Biochem. J. 390:285-292(2005) [PubMed] [Europe PMC] [Abstract]
"Phosphoproteomic analysis of the human pituitary."
Pituitary 9:109-120(2006) [PubMed] [Europe PMC] [Abstract]
"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
"Secreted kinase phosphorylates extracellular proteins that regulate biomineralization."
Science 336:1150-1153(2012) [PubMed] [Europe PMC] [Abstract]
"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins."
J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract]
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]