Neutrophil Specific Antigen 1 (NB1)
CD177; HNA2A; PRV1; HNA-2A; NB1 GP; Polycythemia Rubra Vera 1; Human neutrophil alloantigen 2a; Polycythemia rubra vera protein 1
NB1, a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia .
Independently, Kissel et al. (2001) cloned and characterized NB1, which they also referred to as CD177 and HNA2A. They obtained the cDNA after purification and microsequence analysis of NB1 protein from normal resting granulocytes. Kissel et al. (2001) noted amino acid differences between the PRV1 (Temerinac et al., 2000) and NB1 sequences at positions 3, 119, 323, and 379 and suggested that 2 different, highly homologous genes may exist.By genomic sequence analysis and PCR, Kissel et al. (2002) determined that the NB1 gene contains 9 exons.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV868Hu01 | Recombinant Neutrophil Specific Antigen 1 (NB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV868Hu01 | Monoclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IP. |
| PAV868Hu01 | Polyclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV868Hu01 | CLIA Kit for Neutrophil Specific Antigen 1 (NB1) | CLIA assay for Antigen Detection |
| ELV868Hu01 | ELISA Kit for Neutrophil Specific Antigen 1 (NB1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV868Mu01 | Recombinant Neutrophil Specific Antigen 1 (NB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV868Mu01 | Monoclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IP. |
| PAV868Mu01 | Polyclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV868Mu01 | CLIA Kit for Neutrophil Specific Antigen 1 (NB1) | CLIA assay for Antigen Detection |
| ELV868Mu01 | ELISA Kit for Neutrophil Specific Antigen 1 (NB1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV868Ra01 | Recombinant Neutrophil Specific Antigen 1 (NB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV868Ra01 | Monoclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IP. |
| PAV868Ra01 | Polyclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV868Ra01 | CLIA Kit for Neutrophil Specific Antigen 1 (NB1) | CLIA assay for Antigen Detection |
| ELV868Ra01 | ELISA Kit for Neutrophil Specific Antigen 1 (NB1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV868Ca01 | Recombinant Neutrophil Specific Antigen 1 (NB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV868Ca01 | Monoclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IP. |
| PAV868Ca01 | Polyclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV868Ca01 | CLIA Kit for Neutrophil Specific Antigen 1 (NB1) | CLIA assay for Antigen Detection |
| ELV868Ca01 | ELISA Kit for Neutrophil Specific Antigen 1 (NB1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV868Po01 | Recombinant Neutrophil Specific Antigen 1 (NB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV868Po01 | Monoclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IP. |
| PAV868Po01 | Polyclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV868Po01 | CLIA Kit for Neutrophil Specific Antigen 1 (NB1) | CLIA assay for Antigen Detection |
| ELV868Po01 | ELISA Kit for Neutrophil Specific Antigen 1 (NB1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV868Bo01 | Recombinant Neutrophil Specific Antigen 1 (NB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV868Bo01 | Monoclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IP. |
| PAV868Bo01 | Polyclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV868Bo01 | CLIA Kit for Neutrophil Specific Antigen 1 (NB1) | CLIA assay for Antigen Detection |
| ELV868Bo01 | ELISA Kit for Neutrophil Specific Antigen 1 (NB1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV868Rb01 | Recombinant Neutrophil Specific Antigen 1 (NB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV868Rb01 | Monoclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IP. |
| PAV868Rb01 | Polyclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV868Rb01 | CLIA Kit for Neutrophil Specific Antigen 1 (NB1) | CLIA assay for Antigen Detection |
| ELV868Rb01 | ELISA Kit for Neutrophil Specific Antigen 1 (NB1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV868Mo01 | Recombinant Neutrophil Specific Antigen 1 (NB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV868Mo01 | Monoclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IP. |
| PAV868Mo01 | Polyclonal Antibody to Neutrophil Specific Antigen 1 (NB1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV868Mo01 | CLIA Kit for Neutrophil Specific Antigen 1 (NB1) | CLIA assay for Antigen Detection |
| ELV868Mo01 | ELISA Kit for Neutrophil Specific Antigen 1 (NB1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Cloning of PRV-1, a novel member of the uPAR receptor superfamily, which is overexpressed in polycythemia rubra vera."
Blood 95:2569-2576(2000) [PubMed] [Europe PMC] [Abstract]
"Molecular basis of the neutrophil glycoprotein NB1 (CD177) involved in the pathogenesis of immunenneutropenia and transfusion reactions."
Eur. J. Immunol. 31:1301-1309(2001) [PubMed] [Europe PMC] [Abstract]
"Molecular basis of NB1 (HNA-2a, CD177) deficiency."
Blood 99:4231-4233(2002) [PubMed] [Europe PMC] [Abstract]
"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Signal peptide prediction based on analysis of experimentally verified cleavage sites."
Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract]
"Biochemical characterization of PRV-1, a novel hematopoietic cell surface receptor, which is overexpressed in polycythemia rubra vera."
Blood 100:2441-2448(2002) [PubMed] [Europe PMC] [Abstract]
"Overexpression of the polycythemia rubra vera-1 gene in essential thrombocythemia."
J. Clin. Oncol. 20:4249-4254(2002) [PubMed] [Europe PMC] [Abstract]
"Lack of NB1 GP (CD177/HNA-2a) gene transcription in NB1 GP-neutrophils from NB1 GP-expressing individuals and association of low expression with NB1 gene polymorphisms."
Blood 102:731-733(2003) [PubMed] [Europe PMC] [Abstract]
"CD177 polymorphisms: correlation between high-frequency single nucleotide polymorphisms and neutrophil surface protein expression."
Transfusion 44:77-82(2004) [PubMed] [Europe PMC] [Abstract]