Cytochrome P450 7A1 (CYP7A1)

CYP7; CYP7-A1; CYPVII; Cholesterol 7 Alpha Hydroxylase; Cytochrome P450 Family 7 Subfamily A Polypeptide 1

SPN	V759
HGNC	2651
MGI	106091
PubMed	21391220
RGD	2482
UniProt	P22680
Wiki	CYP7

Cholesterol 7 alpha-hydroxylase (CYP7A1) is the rate-limiting enzyme in the synthesis of bile acid from cholesterol, catalyzing the formation of 7-alpha-hydroxycholesterol. CYP7A1 is a cytochrome P450 heme enzyme that oxidizes cholesterol using molecular oxygen.

It is downregulated by Sterol regulatory element binding proteins (SREBP) when plasma cholesterol levels are low. It is upregulated by the nuclear receptor LXR (liver X receptor) when cholesterol (specifically - oxysterol) levels are high. The effect of this upregulation is to increase the production of bile acids and reduce the level of cholesterol in hepatocytes.

Organism species: Homo sapiens (Human)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPV759Hu01	Recombinant Cytochrome P450 7A1 (CYP7A1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAV759Hu01	Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IP.
Antibodies	PAV759Hu01	Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IF.
Assay Kits	CLV759Hu01	CLIA Kit for Cytochrome P450 7A1 (CYP7A1)	CLIA assay for Antigen Detection
Assay Kits	ELV759Hu01	ELISA Kit for Cytochrome P450 7A1 (CYP7A1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPV759Mu01	Recombinant Cytochrome P450 7A1 (CYP7A1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAV759Mu01	Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IP.
Antibodies	PAV759Mu01	Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IF.
Assay Kits	CLV759Mu01	CLIA Kit for Cytochrome P450 7A1 (CYP7A1)	CLIA assay for Antigen Detection
Assay Kits	ELV759Mu01	ELISA Kit for Cytochrome P450 7A1 (CYP7A1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPV759Ra01	Recombinant Cytochrome P450 7A1 (CYP7A1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAV759Ra01	Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IP.
Antibodies	PAV759Ra01	Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IF.
Assay Kits	CLV759Ra01	CLIA Kit for Cytochrome P450 7A1 (CYP7A1)	CLIA assay for Antigen Detection
Assay Kits	ELV759Ra01	ELISA Kit for Cytochrome P450 7A1 (CYP7A1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPV759Ca01	Recombinant Cytochrome P450 7A1 (CYP7A1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAV759Ca01	Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IP.
Antibodies	PAV759Ca01	Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IF.
Assay Kits	CLV759Ca01	CLIA Kit for Cytochrome P450 7A1 (CYP7A1)	CLIA assay for Antigen Detection
Assay Kits	ELV759Ca01	ELISA Kit for Cytochrome P450 7A1 (CYP7A1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPV759Po01	Recombinant Cytochrome P450 7A1 (CYP7A1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAV759Po01	Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IP.
Antibodies	PAV759Po01	Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IF.
Assay Kits	CLV759Po01	CLIA Kit for Cytochrome P450 7A1 (CYP7A1)	CLIA assay for Antigen Detection
Assay Kits	ELV759Po01	ELISA Kit for Cytochrome P450 7A1 (CYP7A1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPV759Bo01	Recombinant Cytochrome P450 7A1 (CYP7A1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAV759Bo01	Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IP.
Antibodies	PAV759Bo01	Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IF.
Assay Kits	CLV759Bo01	CLIA Kit for Cytochrome P450 7A1 (CYP7A1)	CLIA assay for Antigen Detection
Assay Kits	ELV759Bo01	ELISA Kit for Cytochrome P450 7A1 (CYP7A1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPV759Rb01	Recombinant Cytochrome P450 7A1 (CYP7A1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAV759Rb01	Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IP.
Antibodies	PAV759Rb01	Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IF.
Assay Kits	CLV759Rb01	CLIA Kit for Cytochrome P450 7A1 (CYP7A1)	CLIA assay for Antigen Detection
Assay Kits	ELV759Rb01	ELISA Kit for Cytochrome P450 7A1 (CYP7A1)	Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE	CATALOG NO.	PRODUCT NAME	APPLICATIONS
Proteins	RPV759Mo01	Recombinant Cytochrome P450 7A1 (CYP7A1)	Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies	MAV759Mo01	Monoclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IP.
Antibodies	PAV759Mo01	Polyclonal Antibody to Cytochrome P450 7A1 (CYP7A1)	WB; IHC; ICC; IF.
Assay Kits	CLV759Mo01	CLIA Kit for Cytochrome P450 7A1 (CYP7A1)	CLIA assay for Antigen Detection
Assay Kits	ELV759Mo01	ELISA Kit for Cytochrome P450 7A1 (CYP7A1)	Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Structure of the gene encoding human liver cholesterol 7 alpha-hydroxylase."
Biochim. Biophys. Acta 1172:147-150(1993) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning and sequence analysis of cDNA encoding human cholesterol 7 alpha-hydroxylase."
FEBS Lett. 268:137-140(1990) [PubMed] [Europe PMC] [Abstract]

"Polymorphisms of human cholesterol 7 alpha-hydroxylase."
Biochem. Biophys. Res. Commun. 185:588-595(1992) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Structure and nucleotide sequences of the human cholesterol 7 alpha-hydroxylase gene (CYP7)."
Genomics 20:320-323(1994) [PubMed] [Europe PMC] [Abstract]

"Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene."
Biochemistry 31:2539-2544(1992) [PubMed] [Europe PMC] [Abstract]

"Feedback regulation of bile acid synthesis in human liver: importance of HNF-4alpha for regulation of CYP7A1."
Biochem. Biophys. Res. Commun. 330:395-399(2005) [PubMed] [Europe PMC] [Abstract]

"Glucose stimulates cholesterol 7alpha-hydroxylase gene transcription in human hepatocytes."
J. Lipid Res. 51:832-842(2010) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of human CYP7A1."
Submitted (FEB-2009) to the PDB data bank

"Catalog of 680 variations among eight cytochrome p450 (CYP) genes, nine esterase genes, and two other genes in the Japanese population."
J. Hum. Genet. 48:249-270(2003) [PubMed] [Europe PMC] [Abstract]