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Caspase Activated DNase (CAD)

DFF-B; DFFB; CPAN; DFF40; DFF2; DNA Fragmentation Factor 40kDa Beta Polypeptide; Caspase-activated deoxyribonuclease; Caspase-activated nuclease

DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase), also known as DFFB. Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV754Hu01 Recombinant Caspase Activated DNase (CAD) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV754Hu01 Monoclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IP.
PAV754Hu01 Polyclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IF.
Assay Kits CLV754Hu01 CLIA Kit for Caspase Activated DNase (CAD) CLIA assay for Antigen Detection
ELV754Hu01 ELISA Kit for Caspase Activated DNase (CAD) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV754Mu01 Recombinant Caspase Activated DNase (CAD) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV754Mu01 Monoclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IP.
PAV754Mu01 Polyclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IF.
Assay Kits CLV754Mu01 CLIA Kit for Caspase Activated DNase (CAD) CLIA assay for Antigen Detection
ELV754Mu01 ELISA Kit for Caspase Activated DNase (CAD) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV754Ra01 Recombinant Caspase Activated DNase (CAD) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV754Ra01 Monoclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IP.
PAV754Ra01 Polyclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IF.
Assay Kits CLV754Ra01 CLIA Kit for Caspase Activated DNase (CAD) CLIA assay for Antigen Detection
ELV754Ra01 ELISA Kit for Caspase Activated DNase (CAD) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV754Ca01 Recombinant Caspase Activated DNase (CAD) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV754Ca01 Monoclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IP.
PAV754Ca01 Polyclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IF.
Assay Kits CLV754Ca01 CLIA Kit for Caspase Activated DNase (CAD) CLIA assay for Antigen Detection
ELV754Ca01 ELISA Kit for Caspase Activated DNase (CAD) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV754Po01 Recombinant Caspase Activated DNase (CAD) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV754Po01 Monoclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IP.
PAV754Po01 Polyclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IF.
Assay Kits CLV754Po01 CLIA Kit for Caspase Activated DNase (CAD) CLIA assay for Antigen Detection
ELV754Po01 ELISA Kit for Caspase Activated DNase (CAD) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV754Bo01 Recombinant Caspase Activated DNase (CAD) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV754Bo01 Monoclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IP.
PAV754Bo01 Polyclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IF.
Assay Kits CLV754Bo01 CLIA Kit for Caspase Activated DNase (CAD) CLIA assay for Antigen Detection
ELV754Bo01 ELISA Kit for Caspase Activated DNase (CAD) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV754Rb01 Recombinant Caspase Activated DNase (CAD) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV754Rb01 Monoclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IP.
PAV754Rb01 Polyclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IF.
Assay Kits CLV754Rb01 CLIA Kit for Caspase Activated DNase (CAD) CLIA assay for Antigen Detection
ELV754Rb01 ELISA Kit for Caspase Activated DNase (CAD) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPV754Mo01 Recombinant Caspase Activated DNase (CAD) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAV754Mo01 Monoclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IP.
PAV754Mo01 Polyclonal Antibody to Caspase Activated DNase (CAD) WB; IHC; ICC; IF.
Assay Kits CLV754Mo01 CLIA Kit for Caspase Activated DNase (CAD) CLIA assay for Antigen Detection
ELV754Mo01 ELISA Kit for Caspase Activated DNase (CAD) Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis."
Proc. Natl. Acad. Sci. U.S.A. 95:8461-8466(1998) [PubMed] [Europe PMC] [Abstract]

"Molecular cloning and characterization of human caspase-activated DNase."
Proc. Natl. Acad. Sci. U.S.A. 95:9123-9128(1998) [PubMed] [Europe PMC] [Abstract]

"CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45."
Curr. Biol. 8:537-540(1998) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"An unappreciated role for RNA surveillance."
Genome Biol. 5:R8.1-R8.16(2004) [PubMed] [Europe PMC] [Abstract]

"Histone H1 subtype preferences of DFF40 and possible nuclear localization of DFF40/45 in normal and trichostatin A-treated NB4 leukemic cells."
Apoptosis 15:128-138(2010) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"Structure and mutation analysis of the gene encoding DNA fragmentation factor 40 (caspase-activated nuclease), a candidate neuroblastoma tumour suppressor gene."
Hum. Genet. 106:406-413(2000) [PubMed] [Europe PMC] [Abstract]