Elastase 4 (ELA4)
CLCR; CTRC; Chymotrypsin C; Caldecrin
- Elastase 4 isa member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. These human caldecrin isoforms, like the rat preproenzyme, are composed of a signal peptide of 16 amino acids, a propeptide of 13 amino acids, and a mature form of 239 amino acids. Both recombinant caldecrins, produced in a baculovirus expression system, showed the same chymotrypsin-type protease activity and hypocalcemic activity. The hypocalcemic activity of both caldecrin isoforms remained intact even after treatment with phenylmethylsulfonyl fluoride (PMSF) to abolish their protease activity.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV522Hu01 | Recombinant Elastase 4 (ELA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV522Hu01 | Monoclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IP. |
| PAV522Hu01 | Polyclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV522Hu01 | CLIA Kit for Elastase 4 (ELA4) | CLIA assay for Antigen Detection |
| ELV522Hu01 | ELISA Kit for Elastase 4 (ELA4) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV522Mu01 | Recombinant Elastase 4 (ELA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV522Mu01 | Monoclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IP. |
| PAV522Mu01 | Polyclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV522Mu01 | CLIA Kit for Elastase 4 (ELA4) | CLIA assay for Antigen Detection |
| ELV522Mu01 | ELISA Kit for Elastase 4 (ELA4) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV522Ra01 | Recombinant Elastase 4 (ELA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV522Ra01 | Monoclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IP. |
| PAV522Ra01 | Polyclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV522Ra01 | CLIA Kit for Elastase 4 (ELA4) | CLIA assay for Antigen Detection |
| ELV522Ra01 | ELISA Kit for Elastase 4 (ELA4) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV522Ca01 | Recombinant Elastase 4 (ELA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV522Ca01 | Monoclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IP. |
| PAV522Ca01 | Polyclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV522Ca01 | CLIA Kit for Elastase 4 (ELA4) | CLIA assay for Antigen Detection |
| ELV522Ca01 | ELISA Kit for Elastase 4 (ELA4) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV522Po01 | Recombinant Elastase 4 (ELA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV522Po01 | Monoclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IP. |
| PAV522Po01 | Polyclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV522Po01 | CLIA Kit for Elastase 4 (ELA4) | CLIA assay for Antigen Detection |
| ELV522Po01 | ELISA Kit for Elastase 4 (ELA4) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV522Bo01 | Recombinant Elastase 4 (ELA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV522Bo01 | Monoclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IP. |
| PAV522Bo01 | Polyclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV522Bo01 | CLIA Kit for Elastase 4 (ELA4) | CLIA assay for Antigen Detection |
| ELV522Bo01 | ELISA Kit for Elastase 4 (ELA4) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV522Rb01 | Recombinant Elastase 4 (ELA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV522Rb01 | Monoclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IP. |
| PAV522Rb01 | Polyclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV522Rb01 | CLIA Kit for Elastase 4 (ELA4) | CLIA assay for Antigen Detection |
| ELV522Rb01 | ELISA Kit for Elastase 4 (ELA4) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV522Mo01 | Recombinant Elastase 4 (ELA4) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV522Mo01 | Monoclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IP. |
| PAV522Mo01 | Polyclonal Antibody to Elastase 4 (ELA4) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV522Mo01 | CLIA Kit for Elastase 4 (ELA4) | CLIA assay for Antigen Detection |
| ELV522Mo01 | ELISA Kit for Elastase 4 (ELA4) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Molecular cloning and expression of human caldecrin."
FEBS Lett. 386:26-28(1996) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"The DNA sequence and biological annotation of human chromosome 1."
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Caldecrin is a novel-type serine protease expressed in pancreas, but its homologue, elastase IV, is an artifact during cloning derived from caldecrin gene."
J. Biochem. 123:546-554(1998) [PubMed] [Europe PMC] [Abstract]
"Long-range electrostatic complementarity governs substrate recognition by human chymotrypsin C, a key regulator of digestive enzyme activation."
J. Biol. Chem. 288:9848-9859(2013) [PubMed] [Europe PMC] [Abstract]
"Association of rare chymotrypsinogen C (CTRC) gene variations in patients with idiopathic chronic pancreatitis."
Hum. Genet. 123:83-91(2008) [PubMed] [Europe PMC] [Abstract]
"Chymotrypsin C (CTRC) variants that diminish activity or secretion are associated with chronic pancreatitis."
Nat. Genet. 40:78-82(2008) [PubMed] [Europe PMC] [Abstract]
"Association of novel chymotrypsin C gene variations and haplotypes in patients with chronic pancreatitis in Chinese in Taiwan."
Pancreatology 9:287-292(2009) [PubMed] [Europe PMC] [Abstract]
"Identification of novel missense CTRC variants in Japanese patients with chronic pancreatitis."
Gut 62:653-654(2013) [PubMed] [Europe PMC] [Abstract]
"Comprehensive screening of chymotrypsin C (CTRC) gene in tropical calcific pancreatitis identifies novel variants."
Gut 62:1602-1606(2013) [PubMed] [Europe PMC] [Abstract]
"Comprehensive functional analysis of chymotrypsin C (CTRC) variants reveals distinct loss-of-function mechanisms associated with pancreatitis risk."
Gut 62:1616-1624(2013) [PubMed] [Europe PMC] [Abstract]