General Transcription Factor IIF, Polypeptide 1 (GTF2F1)

GTF2-F1; BTF4; RAP74; TF2F1; TFIIF; General transcription factor IIF 74 kDa subunit

SPN	V164
HGNC	4652
MGI	1923848
PubMed	8486367
RGD	1359646
UniProt	P35269
Wiki	GTF2F1

Transcription factor II F (TF_IIF) is one of several general transcription factors that make up the RNA polymerase II preinitiation complex.Transcription Factor II F is encoded by the GTF2F1, GTF2F2, and GTF2F2L genes.DNA is thought to be wrapped one complete turn around the preinitiation complex and it is TFIIF that helps keep this tight wrapping. In the process, the torsional strain on the DNA may aid in DNA melting at the promoter, forming the transcription bubble. Of all the general transcription factors, TFIIF is the most similar to bacterial sigma factor.

At least 6 chromatographically resolvable general transcription factors may participate in accurate initiation by RNA polymerase II in HeLa cell-derived systems.

Reference

"Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF."
Nature 355:461-464(1992) [PubMed] [Europe PMC] [Abstract]

"A cDNA encoding RAP74, a general initiation factor for transcription by RNA polymerase II."
Nature 355:464-467(1992) [PubMed] [Europe PMC] [Abstract]

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Evolutionary conservation of human TATA-binding-polypeptide-associated factors TAFII31 and TAFII80 and interactions of TAFII80 with other TAFs and with general transcription factors."
Proc. Natl. Acad. Sci. U.S.A. 92:8195-8199(1995) [PubMed] [Europe PMC] [Abstract]

"TAFII250 is a bipartite protein kinase that phosphorylates the base transcription factor RAP74."
Cell 84:781-790(1996) [PubMed] [Europe PMC] [Abstract]

"Kinase activity and phosphorylation of the largest subunit of TFIIF transcription factor."
J. Biol. Chem. 274:22387-22392(1999) [PubMed] [Europe PMC] [Abstract]

"Interaction with general transcription factor IIF (TFIIF) is required for the suppression of activated transcription by RPB5-mediating protein (RMP)."
Cell Res. 13:111-120(2003) [PubMed] [Europe PMC] [Abstract]

"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]

"Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal differential cellular gene expression in response to enterovirus 71 infection."
Cell. Microbiol. 8:565-580(2006) [PubMed] [Europe PMC] [Abstract]

"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]

"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]

"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]

"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Novel dimerization fold of RAP30/RAP74 in human TFIIF at 1.7 A resolution."
J. Mol. Biol. 302:1119-1127(2000) [PubMed] [Europe PMC] [Abstract]

"Crystal structure of the C-terminal domain of the RAP74 subunit of human transcription factor IIF."
Proc. Natl. Acad. Sci. U.S.A. 98:3115-3120(2001) [PubMed] [Europe PMC] [Abstract]

"Molecular mechanism of recruitment of TFIIF-associating RNA polymerase C-terminal domain phosphatase (FCP1) by transcription factor IIF."
Proc. Natl. Acad. Sci. U.S.A. 100:2296-2299(2003) [PubMed] [Europe PMC] [Abstract]

"Solution structure of the carboxyl-terminal domain of RAP74 and NMR characterization of the FCP1-binding sites of RAP74 and human TFIIB."
Biochemistry 42:1460-1469(2003) [PubMed] [Europe PMC] [Abstract]

"NMR structure of a complex containing the TFIIF subunit RAP74 and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1."
Proc. Natl. Acad. Sci. U.S.A. 100:5688-5693(2003) [PubMed] [Europe PMC] [Abstract]