Protein Inhibitor Of Activated STAT 1 (PIAS1)
GBP; DDXBP1; GU/RH-II; ZMIZ3; Gu-binding protein; Zinc Finger,MIZ-Type Containing 3; DEAD/H(Asp-Glu-Ala-Asp/His)Box Binding Protein 1; RNA helicase II-binding protein
STAT proteins are latent cytoplasmic transcription factors that become activated by tyrosine phosphorylation in response to cytokine stimulation.
E3 SUMO-protein ligase PIAS1 is a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene activation and the DNA binding activity, binds to Gu protein/RNA helicase II/DEAD box polypeptide 21, and interacts with androgen receptor (AR). It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in AR initiation and maintenance of spermatogenesis.
Organism species: Homo sapiens (Human)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV103Hu01 | Recombinant Protein Inhibitor Of Activated STAT 1 (PIAS1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV103Hu01 | Monoclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IP. |
| PAV103Hu01 | Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV103Hu01 | CLIA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | CLIA assay for Antigen Detection |
| ELV103Hu01 | ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Mus musculus (Mouse)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV103Mu01 | Recombinant Protein Inhibitor Of Activated STAT 1 (PIAS1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV103Mu01 | Monoclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IP. |
| PAV103Mu01 | Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV103Mu01 | CLIA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | CLIA assay for Antigen Detection |
| ELV103Mu01 | ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rattus norvegicus (Rat)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV103Ra01 | Recombinant Protein Inhibitor Of Activated STAT 1 (PIAS1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV103Ra01 | Monoclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IP. |
| PAV103Ra01 | Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV103Ra01 | CLIA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | CLIA assay for Antigen Detection |
| ELV103Ra01 | ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Canis familiaris; Canine (Dog)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV103Ca01 | Recombinant Protein Inhibitor Of Activated STAT 1 (PIAS1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV103Ca01 | Monoclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IP. |
| PAV103Ca01 | Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV103Ca01 | CLIA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | CLIA assay for Antigen Detection |
| ELV103Ca01 | ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Sus scrofa; Porcine (Pig)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV103Po01 | Recombinant Protein Inhibitor Of Activated STAT 1 (PIAS1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV103Po01 | Monoclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IP. |
| PAV103Po01 | Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV103Po01 | CLIA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | CLIA assay for Antigen Detection |
| ELV103Po01 | ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Bos taurus; Bovine (Cattle)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV103Bo01 | Recombinant Protein Inhibitor Of Activated STAT 1 (PIAS1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV103Bo01 | Monoclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IP. |
| PAV103Bo01 | Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV103Bo01 | CLIA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | CLIA assay for Antigen Detection |
| ELV103Bo01 | ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Oryctolagus cuniculus (Rabbit)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV103Rb01 | Recombinant Protein Inhibitor Of Activated STAT 1 (PIAS1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV103Rb01 | Monoclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IP. |
| PAV103Rb01 | Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV103Rb01 | CLIA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | CLIA assay for Antigen Detection |
| ELV103Rb01 | ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Organism species: Rhesus monkey (Simian)
| PRODUCT TYPE | CATALOG NO. | PRODUCT NAME | APPLICATIONS |
|---|---|---|---|
| Proteins | RPV103Mo01 | Recombinant Protein Inhibitor Of Activated STAT 1 (PIAS1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | MAV103Mo01 | Monoclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IP. |
| PAV103Mo01 | Polyclonal Antibody to Protein Inhibitor Of Activated STAT 1 (PIAS1) | WB; IHC; ICC; IF. | |
| Assay Kits | CLV103Mo01 | CLIA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | CLIA assay for Antigen Detection |
| ELV103Mo01 | ELISA Kit for Protein Inhibitor Of Activated STAT 1 (PIAS1) | Enzyme-linked immunosorbent assay for Antigen Detection |
Reference
"Inhibition of Stat1-mediated gene activation by PIAS1."
Proc. Natl. Acad. Sci. U.S.A. 95:10626-10631(1998) [PubMed] [Europe PMC] [Abstract]
"Protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1) is a nuclear receptor coregulator expressed in human testis."
Mol. Endocrinol. 14:14-26(2000) [PubMed] [Europe PMC] [Abstract]
"Discovery of novel human transcript variants by analysis of intronic single-block EST with polyadenylation site."
BMC Genomics 10:518-518(2009) [PubMed] [Europe PMC] [Abstract]
"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
"Analysis of the DNA sequence and duplication history of human chromosome 15."
Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
"Cloning and characterization of Gu/RH-II binding protein."
Biochem. Biophys. Res. Commun. 234:335-340(1997) [PubMed] [Europe PMC] [Abstract]
"LIM-domain protein cysteine- and glycine-rich protein 2 (CRP2) is a novel marker of hepatic stellate cells and binding partner of the protein inhibitor of activated STAT1."
Biochem. J. 359:485-496(2001) [PubMed] [Europe PMC] [Abstract]
"Involvement of PIAS1 in the sumoylation of tumor suppressor p53."
Mol. Cell 8:713-718(2001) [PubMed] [Europe PMC] [Abstract]
"Distinct effects of PIAS proteins on androgen-mediated gene activation in prostate cancer cells."
Oncogene 20:3880-3887(2001) [PubMed] [Europe PMC] [Abstract]
"SUMO-1 modification of the C-terminal KVEKVD of Axin is required for JNK activation but has no effect on Wnt signaling."
J. Biol. Chem. 277:42981-42986(2002) [PubMed] [Europe PMC] [Abstract]
"Sumoylation of Mdm2 by protein inhibitor of activated STAT (PIAS) and RanBP2 enzymes."
J. Biol. Chem. 277:50131-50136(2002) [PubMed] [Europe PMC] [Abstract]
"Transcription factor Sp3 is silenced through SUMO modification by PIAS1."
EMBO J. 21:5206-5215(2002) [PubMed] [Europe PMC] [Abstract]
"Members of the PIAS family act as SUMO ligases for c-Jun and p53 and repress p53 activity."
Proc. Natl. Acad. Sci. U.S.A. 99:2872-2877(2002) [PubMed] [Europe PMC] [Abstract]
"SUMO modification of a novel MAR-binding protein, SATB2, modulates immunoglobulin mu gene expression."
Genes Dev. 17:3048-3061(2003) [PubMed] [Europe PMC] [Abstract]
"PIAS1-mediated sumoylation of focal adhesion kinase activates its autophosphorylation."
J. Biol. Chem. 278:47434-47440(2003) [PubMed] [Europe PMC] [Abstract]
"Repression of the transactivating capacity of the oncoprotein PLAG1 by SUMOylation."
J. Biol. Chem. 279:36121-36131(2004) [PubMed] [Europe PMC] [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
"Sumoylation delimits KLF8 transcriptional activity associated with the cell cycle regulation."
J. Biol. Chem. 281:16664-16671(2006) [PubMed] [Europe PMC] [Abstract]
"Proinflammatory stimuli induce IKKalpha-mediated phosphorylation of PIAS1 to restrict inflammation and immunity."
Cell 129:903-914(2007) [PubMed] [Europe PMC] [Abstract]
"SUMO modification of the DEAD box protein p68 modulates its transcriptional activity and promotes its interaction with HDAC1."
Oncogene 26:5866-5876(2007) [PubMed] [Europe PMC] [Abstract]
"A quantitative atlas of mitotic phosphorylation."
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
"PRMT1-mediated arginine methylation of PIAS1 regulates STAT1 signaling."
Genes Dev. 23:118-132(2009) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
"SUMOylation and SUMO-interacting motif (SIM) of metastasis tumor antigen 1 (MTA1) synergistically regulate its transcriptional repressor function."
J. Biol. Chem. 286:43793-43808(2011) [PubMed] [Europe PMC] [Abstract]
"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
"NMR structure of the N-terminal domain of SUMO ligase PIAS1 and its interaction with tumor suppressor p53 and A/T-rich DNA oligomers."
J. Biol. Chem. 279:31455-31461(2004) [PubMed] [Europe PMC] [Abstract]