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Calpain 1, Large Subunit (CAPN1)

PIG30; CANPL1; MuCANP; MuCL; Calpain-1 Catalytic Subunit; Calcium-Dependent Protease 1; Calcium-activated neutral proteinase 1; Calpain mu-type; Micromolar-calpain

Calpain (calcium-dependent protease) is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 80 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit (CAPNS1), with the differences arising from the L subunits, L1 (CAPN1) and L2 (CAPN2). The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. Calpain-1 catalytic subunit is the large subunit of the ubiquitous enzyme, calpain 1.

Organism species: Homo sapiens (Human)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU878Hu01 Recombinant Calpain 1, Large Subunit (CAPN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU878Hu01 Monoclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IP.
PAU878Hu01 Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IF.
Assay Kits CLU878Hu01 CLIA Kit for Calpain 1, Large Subunit (CAPN1) CLIA assay for Antigen Detection
ELU878Hu01 ELISA Kit for Calpain 1, Large Subunit (CAPN1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU878Mu01 Recombinant Calpain 1, Large Subunit (CAPN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU878Mu01 Monoclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IP.
PAU878Mu01 Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IF.
Assay Kits CLU878Mu01 CLIA Kit for Calpain 1, Large Subunit (CAPN1) CLIA assay for Antigen Detection
ELU878Mu01 ELISA Kit for Calpain 1, Large Subunit (CAPN1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU878Ra01 Recombinant Calpain 1, Large Subunit (CAPN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU878Ra01 Monoclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IP.
PAU878Ra01 Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IF.
Assay Kits CLU878Ra01 CLIA Kit for Calpain 1, Large Subunit (CAPN1) CLIA assay for Antigen Detection
ELU878Ra01 ELISA Kit for Calpain 1, Large Subunit (CAPN1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Canis familiaris; Canine (Dog)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU878Ca01 Recombinant Calpain 1, Large Subunit (CAPN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU878Ca01 Monoclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IP.
PAU878Ca01 Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IF.
Assay Kits CLU878Ca01 CLIA Kit for Calpain 1, Large Subunit (CAPN1) CLIA assay for Antigen Detection
ELU878Ca01 ELISA Kit for Calpain 1, Large Subunit (CAPN1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Sus scrofa; Porcine (Pig)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU878Po01 Recombinant Calpain 1, Large Subunit (CAPN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU878Po01 Monoclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IP.
PAU878Po01 Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IF.
Assay Kits CLU878Po01 CLIA Kit for Calpain 1, Large Subunit (CAPN1) CLIA assay for Antigen Detection
ELU878Po01 ELISA Kit for Calpain 1, Large Subunit (CAPN1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Bos taurus; Bovine (Cattle)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU878Bo01 Recombinant Calpain 1, Large Subunit (CAPN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU878Bo01 Monoclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IP.
PAU878Bo01 Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IF.
Assay Kits CLU878Bo01 CLIA Kit for Calpain 1, Large Subunit (CAPN1) CLIA assay for Antigen Detection
ELU878Bo01 ELISA Kit for Calpain 1, Large Subunit (CAPN1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Oryctolagus cuniculus (Rabbit)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU878Rb01 Recombinant Calpain 1, Large Subunit (CAPN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU878Rb01 Monoclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IP.
PAU878Rb01 Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IF.
Assay Kits CLU878Rb01 CLIA Kit for Calpain 1, Large Subunit (CAPN1) CLIA assay for Antigen Detection
ELU878Rb01 ELISA Kit for Calpain 1, Large Subunit (CAPN1) Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rhesus monkey (Simian)

PRODUCT TYPE CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPU878Mo01 Recombinant Calpain 1, Large Subunit (CAPN1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAU878Mo01 Monoclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IP.
PAU878Mo01 Polyclonal Antibody to Calpain 1, Large Subunit (CAPN1) WB; IHC; ICC; IF.
Assay Kits CLU878Mo01 CLIA Kit for Calpain 1, Large Subunit (CAPN1) CLIA assay for Antigen Detection
ELU878Mo01 ELISA Kit for Calpain 1, Large Subunit (CAPN1) Enzyme-linked immunosorbent assay for Antigen Detection

Reference

"Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence."
FEBS Lett. 205:313-317(1986) [PubMed] [Europe PMC] [Abstract]

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Biol. Chem. Hoppe-Seyler 371:171-176(1990) [PubMed] [Europe PMC] [Abstract]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

"Modulation of the calpain autoproteolysis by calpastatin and phospholipids."
Biochem. Biophys. Res. Commun. 229:193-197(1996) [PubMed] [Europe PMC] [Abstract]

"Autolysis of human erythrocyte calpain produces two active enzyme forms with different cell localization."
FEBS Lett. 392:11-15(1996) [PubMed] [Europe PMC] [Abstract]

"Calcium-binding properties of human erythrocyte calpain."
Biochem. J. 325:721-726(1997) [PubMed] [Europe PMC] [Abstract]

"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]

"Initial characterization of the human central proteome."
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]

"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]

"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]

"An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]

"Molecular mode of action of a covalently inhibiting peptidomimetic on the human calpain protease core."
Biochemistry 45:701-708(2006) [PubMed] [Europe PMC] [Abstract]